INBRE: URI: CENTRALIZED RES CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 中央研究核心设施是在其第五年提供研究和培训支持RI-INBRE参与者和罗得岛生物医学研究界。在本年度，超过53个研究小组使用了该设施，平均每季度使用次数约为1106次。该设施的用户包括12名教师，17名博士后研究员，57名研究生和29名本科生。核心设施的工作人员对所有新用户进行了培训，使他们能够利用各种技术开展研究项目。该设施的工作人员还参加了药学院提供的本科生和研究生实验室课程的各种技术教学。此外，还与仪器制造商合作举办了几次教学研讨会和培训讲习班。今年的主题包括：新型TaqMan阵列和microRNA研究工具; siRNA和miRNA的基因沉默;真实的时间PCR的新概念;电泳2DE和2D-DIGE：理论和实践;以及DNA，RNA，蛋白质和药物的快速有效提取。 购买了QSTAR Elite混合LC/MS/MS系统，以替代Mariner LC/MS系统。 此外，升级了生物分析仪系统的软件。核心设施的设备和资源继续提供给研究所伙伴机构的研究人员。 由于所有参与机构都为维护该设施提供了资金，因此不收取设备使用费。 今年，海报印刷服务为各种调查人员印刷了268张海报。 这项服务的收费将很快实施，以收回消耗品的成本。 总体而言，核心基金的使用继续增加。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Centralized Research Core Facility is in its fifth year of providing research and training support to the RI-INBRE participants and the Rhode Island biomedical research community. During the current year more than fifty three research groups used the facility, with an average frequency of approximately 1106 total usages per quarter. The Facility users consisted of 12 faculty, 17 postdoctoral fellows, 57 graduate students, and 29 undergraduate students. The Core Facility staff trained all new users in utilizing various technologies for their research projects. The Facility staff also participated in teaching various techniques in the undergraduate and graduate laboratory courses offered by the College of Pharmacy. In addition, several instructional seminars and training workshops were organized in collaboration with the instrument manufacturers. This year, the topics included the following: Novel TaqMan Array and microRNA Research Tools; Gene Silencing with siRNA and miRNA; New Concepts in Real Time PCR; Electrophoresis 2DE and 2D-DIGE: Theory and Practice; and Fast and Efficient Extraction of DNA, RNA, Proteins and Drugs. A QSTAR Elite Hybrid LC/MS/MS System was purchased to replace the Mariner LC/MS System. Also, the software for the Bioanalyzer System was upgrade. The Core Facility equipment and resources continue to remain available to researchers from the RI-INBRE partner institutions. No user fees were charged for equipment usage as all participating institutions contributed funds toward maintaining the Facility. This year, the poster printing service printed 268 posters for various investigators. A fee for this service will be implemented soon to recover the cost of consumables. Overall, the usage of the Core Facility continues to increase.