INBRE: URI: CENTRALIZED RES CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 中央研究核心设施是在其第四年提供研究和培训支持RI-INBRE参与者和罗得岛生物医学研究界。核心设施的设备和资源继续提供给来自所有罗得岛学术和研究机构的研究人员，不收取任何使用费。在本年度，核心设施仪器和服务的使用量翻了一番。超过35个研究小组使用了该设施，平均每季度使用次数约为800次。动手用户包括6名教师，14名博士后研究员，36名研究生和25名本科生。所有新用户都接受了核心设施工作人员的培训，学习如何正确利用各种技术开展研究项目。该设施的工作人员还参加了药学院提供的本科生和研究生实验室课程的教学。此外，还与仪器制造商合作举办了几次教学研讨会和培训讲习班。主题包括以下内容：细胞内Western分析的红外成像;基本流式细胞术;实时定量PCR和RT-PCR。今年采购的设备和软件包包括：用于蛋白质组学的Offgel分馏系统; PD-Quest 2D凝胶分析软件;带恒温自动进样器的分析型HPLC系统;以及Milli-Q水净化系统。核心基金的海报印刷服务今年已免费印制了290张海报，供成员机构的调查人员使用。目前正在探讨这项服务的收费结构和机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Centralized Research Core Facility is in its fourth year of providing research and training support to the RI-INBRE participants and the Rhode Island biomedical research community. The Core Facility equipment and resources continue to be made available to researchers from all Rhode Island academic and research institutions without charging any user fees. During the current year, the usage of Core Facility instrumentation and services has doubled. More than thirty five research groups used the Facility, with an average frequency of approximately 800 usages per quarter. The hands-on users included 6 faculty, 14 postdoctoral fellows, 36 graduate students, and 25 undergraduate students. All new users were trained by the Core Facility staff in properly utilizing various technologies for their research projects. The Facility staff also participated in teaching undergraduate and graduate laboratory courses offered by the College of Pharmacy. In addition, several instructional seminars and training workshops were organized in collaboration with the instrument manufacturers. The topics included the following: IR-Imaging for In-Cell Western Analysis; Basic Flow Cytometry; and Real-Time Quantitative PCR and RT-PCR. Equipment and software packages acquired this year included the following: Offgel Fractionation System for Proteomics; PD-Quest 2D Gel Analysis Software; Analytical HPLC System with Thermostatic Auto-Sampler; and Milli-Q Water Purification System. The poster printing service at the Core Facility has already printed 290 posters this year, free of charge, for investigators from the member institutions. A fee structure and a mechanism to charge for this service is being explored.