INBRE: URI: CENTRALIZED RES CORE FACILITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。中央研究核心设施为RI-INBRE成员和罗德岛生物医学研究界提供研究和培训支持已进入第三个年头。一个空缺的研究助理职位最近已被填补。在过去的一年里，超过20个研究小组使用了该设施，平均每季度使用400次。除教师外，用户还包括14名博士后、40名研究生和23名本科生。新用户接受了利用各种技术进行研究项目的培训。利用核心设施，设施工作人员还参与了药学院提供的本科和研究生实验课程的教学。与仪器制造商合作组织了几次教学和培训研讨会，如实时PCR和傅立叶变换红外光谱法。为了建立一个新的基因转染设施，已经获得了设备，包括培养箱、摇培养箱、PCR和水平凝胶电泳系统。此外，现有的倒置显微镜将升级为数字图像处理。今年获得的其他设备和软件包包括：NanoDrop荧光光谱仪、旋转蒸发器和用于DNA微阵列系统的Rosetta Resolver软件。核心设施的设备和资源继续提供给罗德岛所有学术和研究机构的研究人员，不支付任何使用费。截止到今年2月，印刷公司已经为会员机构的调查人员印刷了200多张海报。计划于2006年4月举办为期一天的流式细胞术研讨会。与以往的教学研讨会一样，本次研讨会将向所有RI-INBRE合作机构的学生和研究人员开放。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Centralized Research Core Facility is in its third year of providing research and training support to the RI-INBRE members and the Rhode Island biomedical research community. A vacant research associate position has recently been filled. During the past year, more than 20 research groups used the Facility, with an average frequency of 400 usages per quarter. Besides the faculty, the users included 14 postdoctoral fellows, 40 graduate students, and 23 undergraduate students. The new users were trained in utilizing various technologies for their research projects. Utilizing the Core Facility, the Facility staff also participated in teaching undergraduate and graduate laboratory courses offered by the College of Pharmacy. Several instructional and training seminars were organized in collaboration with instrument manufacturers, such as Real-Time PCR and Fourier Transformed Infrared Spectrometry. To set up a new gene transfection facility, equipment has been acquired, consisting of incubator, shaking incubator, PCR, and horizontal gel electrophoresis system. In addition, the existing inverted microscope will be upgraded for digital image processing. Other equipment and software packages acquired this year include: a NanoDrop fluorospectrometer, a rotary evaporator, and Rosetta Resolver software for the DNA microarray system. The Core Facility equipment and it's resources continue to be made available to researchers from all Rhode Island academic and research institutions, without paying any user fees. As of February 2006, the printing service has already printed about 200 posters this year for investigators from the member institutions. In April 2006, a one-day workshop on flow cytometry is scheduled. Like the previous instructional workshops, this workshop will be open to students and investigators from all RI-INBRE partner institutions.