Physical mapping of Aedes aegypti genome

埃及伊蚊基因组的物理作图

基本信息

  • 批准号:
    8137887
  • 负责人:
  • 金额:
    $ 22.75万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-09-03 至 2013-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Mosquito-borne diseases have a devastating impact on public health around the world. The yellow fever mosquito, Aedes aegypti, is the primary global vector for dengue virus transmission. Because of its epidemiological importance, Ae. aegypti is one of the most intensively studied mosquitoes in various aspects of genetics, vector-pathogen interactions, and transgenic research. Recent publication of the complete genome sequence for Ae. aegypti has stimulated research in vector biology and insect genomics. However, a lack of high-quality genome assembly placement to chromosome position remains a significant impediment to further research progress. The absence of readable polytene chromosomes makes physical mapping for this mosquito extremely challenging. The current physical map has relatively low resolution and includes ~180 physical markers. Only a third of the Ae. aegypti supercontig assemblies has been assigned to chromosomes, but without linear orders and orientation. We have recently discovered that prometaphase chromosomes from imaginal discs of 4th instar larvae can be an excellent source for the physical mapping of the Ae. aegypti genome for the following reasons: large size of the chromosomes, high numbers of mitotic phase chromosomes on each slide preparation, and the reproducible banding pattern of the individual chromosomes. The specific aims for this project are to 1. Develop a cytogenetic map for Ae. aegypti based on banded prometaphase chromosomes from imaginal discs of 4th instar larvae. 2. Place about 70% of the genome supercontig assemblies to their precise chromosomal locations. This developmental project will modernize cytogenic research for Ae. aegypti and greatly improve the current fragmented genome assembly. We believe that the availability of a high-resolution physical map will accelerate progress in a number of ongoing projects with Ae. aegypti, as well as enhance vector biology and insect genomics overall, and will further stimulate research in mosquito systematics, population genetics, and insect comparative genomics. PUBLIC HEALTH RELEVANCE: Aedes aegypti is the primary global vector for dengue virus. Dengue is an emerging health threat throughout subtropical and tropical regions around the world. No vaccines or drug treatments are available; thus, disease prevention is largely based on efforts to control or avoid contact with infected mosquitoes. The proposed research will develop new genome tools that will greatly enhance the utility of the existing draft genome sequence assembly for Ae. aegypti and thereby facilitate application of advanced genome technologies for investigating and developing novel genetic control strategies for dengue transmission.
描述(由申请人提供):蚊子传播的疾病对世界各地的公共卫生产生破坏性影响。黄热病蚊子,埃及伊蚊,是登革热病毒传播的主要全球载体。由于其流行病学的重要性,Ae。埃及伊蚊是在遗传学、病媒-病原体相互作用和转基因研究的各个方面得到最深入研究的蚊子之一。最近出版的完整基因组序列的Ae。埃及促进了媒介生物学和昆虫基因组学的研究。然而,缺乏高质量的基因组组装放置到染色体位置仍然是进一步研究进展的重大障碍。由于缺乏可读的多线染色体,这种蚊子的物理作图极具挑战性。目前的物理地图分辨率相对较低,包括约180个物理标记。只有三分之一的阿。埃及超重叠群组装体已被分配给染色体,但没有线性顺序和方向。我们最近发现,前中期染色体从4龄幼虫的成虫盘可以是一个很好的来源,为物理作图的Ae。由于以下原因,埃及人的基因组中存在大量的染色体:染色体尺寸大,每个载玻片制备物上的有丝分裂期染色体数量多,以及单个染色体的可再现带型。该项目的具体目标是1。绘制Ae的细胞遗传学图谱。埃及伊蚊基于4龄幼虫的成虫盘的带状前中期染色体。2.将约70%的基因组超重叠群组装体放置到其精确的染色体位置。这一发展项目将使Ae的细胞遗传学研究现代化。并极大地改善了目前的片段化基因组组装。我们相信,高分辨率物理地图的可用性将加速Ae许多正在进行的项目的进展。埃及伊蚊的研究,并全面加强病媒生物学和昆虫基因组学,还将进一步促进蚊子系统学、种群遗传学和昆虫比较基因组学方面的研究。 公共卫生相关性:埃及伊蚊是登革热病毒的主要全球传播媒介。登革热是一种新兴的健康威胁,遍布世界各地的亚热带和热带地区。没有可用的疫苗或药物治疗;因此,疾病预防主要基于控制或避免接触受感染的蚊子。拟议的研究将开发新的基因组工具,这将大大提高现有的基因组序列组装草图的实用性。埃及伊蚊,从而促进应用先进的基因组技术来调查和开发登革热传播的新的遗传控制策略。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Imaginal discs--a new source of chromosomes for genome mapping of the yellow fever mosquito Aedes aegypti.
  • DOI:
    10.1371/journal.pntd.0001335
  • 发表时间:
    2011-10
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    Sharakhova MV;Timoshevskiy VA;Yang F;Demin SIu;Severson DW;Sharakhov IV
  • 通讯作者:
    Sharakhov IV
Fluorescent in situ hybridization on mitotic chromosomes of mosquitoes.
Genomic composition and evolution of Aedes aegypti chromosomes revealed by the analysis of physically mapped supercontigs.
通过物理定位超重叠群分析揭示埃及伊蚊染色体的基因组组成和进化。
  • DOI:
    10.1186/1741-7007-12-27
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    5.4
  • 作者:
    Timoshevskiy,VladimirA;Kinney,NicholasA;deBruyn,BeckyS;Mao,Chunhong;Tu,Zhijian;Severson,DavidW;Sharakhov,IgorV;Sharakhova,MariaV
  • 通讯作者:
    Sharakhova,MariaV
An integrated linkage, chromosome, and genome map for the yellow fever mosquito Aedes aegypti.
  • DOI:
    10.1371/journal.pntd.0002052
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    Timoshevskiy VA;Severson DW;Debruyn BS;Black WC;Sharakhov IV;Sharakhova MV
  • 通讯作者:
    Sharakhova MV
Heterochromatin, histone modifications, and nuclear architecture in disease vectors.
  • DOI:
    10.1016/j.cois.2015.05.003
  • 发表时间:
    2015-08-01
  • 期刊:
  • 影响因子:
    5.3
  • 作者:
    Sharakhov, Igor V.;Sharakhova, Maria V.
  • 通讯作者:
    Sharakhova, Maria V.
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Maria Sharakhova其他文献

Maria Sharakhova的其他文献

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{{ truncateString('Maria Sharakhova', 18)}}的其他基金

Haplotype-resolved genome assemblies and chromosomal rearrangements in arboviral vector Aedes albopictus
虫媒病毒载体白纹伊蚊中单倍型解析的基因组组装和染色体重排
  • 批准号:
    10573961
  • 财政年份:
    2022
  • 资助金额:
    $ 22.75万
  • 项目类别:
Chromosomal rearrangements in arboviral vector Aedes aegypti
虫媒病毒载体埃及伊蚊的染色体重排
  • 批准号:
    9974917
  • 财政年份:
    2020
  • 资助金额:
    $ 22.75万
  • 项目类别:
Chromosome scale genome assemblies for Culex pipiens mosquitoes
淡色库蚊的染色体规模基因组组装
  • 批准号:
    10302318
  • 财政年份:
    2020
  • 资助金额:
    $ 22.75万
  • 项目类别:
Improving the reference genome assembly for the dengue fever vector Aedes aegypti
改进登革热载体埃及伊蚊的参考基因组组装
  • 批准号:
    9017188
  • 财政年份:
    2016
  • 资助金额:
    $ 22.75万
  • 项目类别:
Chromosome-based genome assembly for Culex quinquefasciatus
基于染色体的致倦库蚊基因组组装
  • 批准号:
    8511874
  • 财政年份:
    2013
  • 资助金额:
    $ 22.75万
  • 项目类别:
Chromosome-based genome assembly for Culex quinquefasciatus
基于染色体的致倦库蚊基因组组装
  • 批准号:
    8604136
  • 财政年份:
    2013
  • 资助金额:
    $ 22.75万
  • 项目类别:
Physical mapping of Aedes aegypti genome
埃及伊蚊基因组的物理作图
  • 批准号:
    7871634
  • 财政年份:
    2010
  • 资助金额:
    $ 22.75万
  • 项目类别:
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