Haplotype-resolved genome assemblies and chromosomal rearrangements in arboviral vector Aedes albopictus

虫媒病毒载体白纹伊蚊中单倍型解析的基因组组装和染色体重排

• 批准号: 10573961
• 负责人: Maria Sharakhova
• 金额: $ 22.71万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-11-08 至 2024-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10573961
• 关键词: Aedes; Americas; Anopheles Genus; Antarctica; Arboviruses; Asia; Asian; Biological; Biology; Breeding; Chikungunya fever; Chinese; Chromosomal Rearrangement; Chromosome inversion; Chromosomes; Climate; Country; Culicidae; DNA Probes; Dangerousness; Dengue; Dengue Fever; Development; Diapause; Disease; Disease Outbreaks; Environment; Epidemic; Epidemiology; Europe; Fluorescent in Situ Hybridization; France; Genes; Genetic; Genetic study; Genome; Genome Mappings; Genomics; Geographic Locations; Goals; Grant; Haplotypes; Health; Hi-C; Hybrids; Infection; Invaded; Italy; Laboratories; Maps; Measures; Methods; Mexico; Mitotic Chromosome; Mosquito Control; Mosquito-borne infectious disease; North America; Panama; Parents; Phenotype; Population; Public Health; Quantitative Trait Loci; Reporting; South American; Southeastern Asia; Southern Europe; Spain; Technology; Testing; Tigers; United States National Institutes of Health; Variant; ZIKA; arboviral disease; chikungunya; climate zone; contig; disease transmission; genome-wide; improved; male; nanopore; new technology; photoperiodicity; physical mapping; prevent; reference genome; scaffold; success; trait; transmission process; vector; vector control; zika fever

PROJECT SUMMARY The Asian tiger mosquito Aedes albopictus is native to Southeast Asia but in a few decades this species expanded its range to all continents except Antarctica. Aedes albopictus is capable of transmitting multiple arboviruses including dengue—the leading arboviral disease of 21st century—Chikungunya, and Zika—an emerging health threats for the world. Because of its remarkable ability to develop a photoperiodic diapause in the temperate climate, Ae. albopictus has the potential to spread these dangerous diseases further north. This project will develop haplotype-resolved chromosome-scale genome assemblies for multiple strains of Ae. albopictus and will test if adaptations to the temperate climate are associated with chromosomal rearrangements in this mosquito. Toward this end, we propose the following specific aims: 1) develop a haplotype-resolved chromosome-scale genome assemblies for four strains of Ae. albopictus including the Foshan strain; 2) improve the physical genome map for the Foshan strain of Ae. albopictus; and 3) identify chromosomal rearrangements in different populations of Ae. albopictus world-wide. Our long- term goal is to understand the genetic basis of the incredible phenotypic plasticity of Ae. albopictus that helps this mosquito to rapidly spread around the globe. We envision that the availability of the high-quality reference genome assemblies for multiple strains of Ae. albopictus will stimulate further genetic studies aimed at preventing mosquito-borne disease transmission.

项目摘要 亚洲虎蚊白纹伊蚊原产于东南亚，但几十年后， 它的范围扩大到除南极洲以外的所有大陆。白纹伊蚊能够传播多种 虫媒病毒，包括登革热-21世纪的主要虫媒病毒疾病-基孔肯雅病毒和寨卡病毒 对世界的健康威胁。由于其显着的能力，发展一个光周期滞育， 温带气候，Ae.白纹伊蚊有可能将这些危险的疾病传播到更北的地方。这 该项目将为多种Ae菌株开发单倍型分辨的染色体规模基因组组装。 白纹伊蚊，并将测试是否适应温带气候与染色体 重新排列。为此，我们提出以下具体目标：1）发展一个 四种Ae菌株的单倍型分辨染色体规模基因组组装。白纹伊蚊，包括 佛山株; 2）改进Ae.白纹伊蚊;以及3） 鉴定不同群体Ae中染色体重排。白纹伊蚊在世界范围内我们长久以来- 长期目标是了解令人难以置信的表型可塑性的遗传基础。白纹伊蚊有助于 这种蚊子在地球仪迅速传播。我们设想，高质量的参考资料的可用性 多株Ae的基因组组装。白纹伊蚊将刺激进一步的基因研究， 预防蚊媒疾病传播。