Haplotype-resolved genome assemblies and chromosomal rearrangements in arboviral vector Aedes albopictus

项目总结 亚洲虎蚊白纹伊蚊原产于东南亚，但几十年后这个物种 将其覆盖范围扩大到除南极洲以外的所有大陆。白纹伊蚊能够传播多个 虫媒病毒，包括登革热--21世纪的主要虫媒病毒病--基孔肯雅，以及寨卡病毒--安 世界面临的新的健康威胁。因为它有非凡的能力来形成光周期滞育 温带气候，Ae.白纹伊蚊有可能将这些危险的疾病进一步向北传播。这 该项目将为多个Ae菌株开发单倍型解析的染色体规模的基因组组件。 并将测试对温带气候的适应是否与染色体有关 这种蚊子的重排。为此，我们提出了以下具体目标：1)开发一种 四株Ae的单倍型解析的染色体规模基因组组装。白纹伊蚊包括 2)改进了Ae佛山株的物理基因组图谱。白纹伊蚊；3) 确定不同Ae群体中的染色体重排。世界各地的白纹伊蚊。我们的长- 学期目标是了解Ae令人难以置信的表型可塑性的遗传基础。白纹伊蚊有帮助 这种蚊子迅速在全球范围内传播。我们预见到高质量参考文献的可用性 多株Ae的基因组组装。白纹伊蚊将刺激进一步的基因研究 防止蚊媒疾病传播。

PROJECT SUMMARY The Asian tiger mosquito Aedes albopictus is native to Southeast Asia but in a few decades this species expanded its range to all continents except Antarctica. Aedes albopictus is capable of transmitting multiple arboviruses including dengue—the leading arboviral disease of 21st century—Chikungunya, and Zika—an emerging health threats for the world. Because of its remarkable ability to develop a photoperiodic diapause in the temperate climate, Ae. albopictus has the potential to spread these dangerous diseases further north. This project will develop haplotype-resolved chromosome-scale genome assemblies for multiple strains of Ae. albopictus and will test if adaptations to the temperate climate are associated with chromosomal rearrangements in this mosquito. Toward this end, we propose the following specific aims: 1) develop a haplotype-resolved chromosome-scale genome assemblies for four strains of Ae. albopictus including the Foshan strain; 2) improve the physical genome map for the Foshan strain of Ae. albopictus; and 3) identify chromosomal rearrangements in different populations of Ae. albopictus world-wide. Our long- term goal is to understand the genetic basis of the incredible phenotypic plasticity of Ae. albopictus that helps this mosquito to rapidly spread around the globe. We envision that the availability of the high-quality reference genome assemblies for multiple strains of Ae. albopictus will stimulate further genetic studies aimed at preventing mosquito-borne disease transmission.