Haplotype-resolved genome assemblies and chromosomal rearrangements in arboviral vector Aedes albopictus

虫媒病毒载体白纹伊蚊中单倍型解析的基因组组装和染色体重排

• 批准号: 10573961
• 负责人: Maria Sharakhova
• 金额: $ 22.71万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-11-08 至 2024-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10573961
• 关键词: Aedes; Americas; Anopheles Genus; Antarctica; Arboviruses; Asia; Asian; Biological; Biology; Breeding; Chikungunya fever; Chinese; Chromosomal Rearrangement; Chromosome inversion; Chromosomes; Climate; Country; Culicidae; DNA Probes; Dangerousness; Dengue; Dengue Fever; Development; Diapause; Disease; Disease Outbreaks; Environment; Epidemic; Epidemiology; Europe; Fluorescent in Situ Hybridization; France; Genes; Genetic; Genetic study; Genome; Genome Mappings; Genomics; Geographic Locations; Goals; Grant; Haplotypes; Health; Hi-C; Hybrids; Infection; Invaded; Italy; Laboratories; Maps; Measures; Methods; Mexico; Mitotic Chromosome; Mosquito Control; Mosquito-borne infectious disease; North America; Panama; Parents; Phenotype; Population; Public Health; Quantitative Trait Loci; Reporting; South American; Southeastern Asia; Southern Europe; Spain; Technology; Testing; Tigers; United States National Institutes of Health; Variant; ZIKA; arboviral disease; chikungunya; climate zone; contig; disease transmission; genome-wide; improved; male; nanopore; new technology; photoperiodicity; physical mapping; prevent; reference genome; scaffold; success; trait; transmission process; vector; vector control; zika fever

PROJECT SUMMARY The Asian tiger mosquito Aedes albopictus is native to Southeast Asia but in a few decades this species expanded its range to all continents except Antarctica. Aedes albopictus is capable of transmitting multiple arboviruses including dengue—the leading arboviral disease of 21st century—Chikungunya, and Zika—an emerging health threats for the world. Because of its remarkable ability to develop a photoperiodic diapause in the temperate climate, Ae. albopictus has the potential to spread these dangerous diseases further north. This project will develop haplotype-resolved chromosome-scale genome assemblies for multiple strains of Ae. albopictus and will test if adaptations to the temperate climate are associated with chromosomal rearrangements in this mosquito. Toward this end, we propose the following specific aims: 1) develop a haplotype-resolved chromosome-scale genome assemblies for four strains of Ae. albopictus including the Foshan strain; 2) improve the physical genome map for the Foshan strain of Ae. albopictus; and 3) identify chromosomal rearrangements in different populations of Ae. albopictus world-wide. Our long- term goal is to understand the genetic basis of the incredible phenotypic plasticity of Ae. albopictus that helps this mosquito to rapidly spread around the globe. We envision that the availability of the high-quality reference genome assemblies for multiple strains of Ae. albopictus will stimulate further genetic studies aimed at preventing mosquito-borne disease transmission.

项目概要 亚洲虎蚊白纹伊蚊原产于东南亚，但几十年后这个物种 将其范围扩大到除南极洲以外的所有大陆。白纹伊蚊能够传播多种 虫媒病毒，包括登革热（21 世纪主要的虫媒病毒疾病——基孔肯雅热）和寨卡病毒（一种 世界新出现的健康威胁。由于其具有显着的光周期滞育能力 温带气候，Ae。白纹伊蚊有可能将这些危险疾病进一步向北传播。这 该项目将为多种伊蚊菌株开发单倍型解析的染色体规模基因组组装。 白纹伊蚊并将测试对温带气候的适应是否与染色体有关 这只蚊子的重新排列。为此，我们提出以下具体目标：1）制定 四种伊蚊菌株的单倍型解析染色体规模基因组组装。白纹伊蚊包括 佛山株； 2）完善了伊蚊佛山株的物理基因组图谱。白纹伊蚊；和 3) 鉴定不同伊蚊种群中的染色体重排。世界范围内的白纹伊蚊。我们的长期 长期目标是了解伊蚊令人难以置信的表型可塑性的遗传基础。白纹伊蚊有帮助 这种蚊子迅速传播到全球。我们预计高质量参考的可用性 多种伊蚊菌株的基因组组装。白纹伊蚊将刺激进一步的遗传学研究 防止蚊媒疾病传播。