Physical mapping of Aedes aegypti genome

埃及伊蚊基因组的物理作图

基本信息

  • 批准号:
    7871634
  • 负责人:
  • 金额:
    $ 20.52万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-09-03 至 2012-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Mosquito-borne diseases have a devastating impact on public health around the world. The yellow fever mosquito, Aedes aegypti, is the primary global vector for dengue virus transmission. Because of its epidemiological importance, Ae. aegypti is one of the most intensively studied mosquitoes in various aspects of genetics, vector-pathogen interactions, and transgenic research. Recent publication of the complete genome sequence for Ae. aegypti has stimulated research in vector biology and insect genomics. However, a lack of high-quality genome assembly placement to chromosome position remains a significant impediment to further research progress. The absence of readable polytene chromosomes makes physical mapping for this mosquito extremely challenging. The current physical map has relatively low resolution and includes ~180 physical markers. Only a third of the Ae. aegypti supercontig assemblies has been assigned to chromosomes, but without linear orders and orientation. We have recently discovered that prometaphase chromosomes from imaginal discs of 4th instar larvae can be an excellent source for the physical mapping of the Ae. aegypti genome for the following reasons: large size of the chromosomes, high numbers of mitotic phase chromosomes on each slide preparation, and the reproducible banding pattern of the individual chromosomes. The specific aims for this project are to 1. Develop a cytogenetic map for Ae. aegypti based on banded prometaphase chromosomes from imaginal discs of 4th instar larvae. 2. Place about 70% of the genome supercontig assemblies to their precise chromosomal locations. This developmental project will modernize cytogenic research for Ae. aegypti and greatly improve the current fragmented genome assembly. We believe that the availability of a high-resolution physical map will accelerate progress in a number of ongoing projects with Ae. aegypti, as well as enhance vector biology and insect genomics overall, and will further stimulate research in mosquito systematics, population genetics, and insect comparative genomics. PUBLIC HEALTH RELEVANCE: Aedes aegypti is the primary global vector for dengue virus. Dengue is an emerging health threat throughout subtropical and tropical regions around the world. No vaccines or drug treatments are available; thus, disease prevention is largely based on efforts to control or avoid contact with infected mosquitoes. The proposed research will develop new genome tools that will greatly enhance the utility of the existing draft genome sequence assembly for Ae. aegypti and thereby facilitate application of advanced genome technologies for investigating and developing novel genetic control strategies for dengue transmission.
描述(申请人提供):蚊子传播的疾病对世界各地的公共卫生造成毁灭性的影响。黄热病蚊子埃及伊蚊是全球传播登革热病毒的主要媒介。由于其在流行病学上的重要性,Ae.埃及伊蚊是遗传学、病媒-病原体相互作用和转基因研究中研究最深入的蚊子之一。最近公布了Ae的全基因组序列。埃及伊蚊刺激了媒介生物学和昆虫基因组学的研究。然而,缺乏高质量的基因组组装放置到染色体位置仍然是进一步研究进展的重大障碍。缺乏可读的多线染色体使这种蚊子的物理图谱极具挑战性。目前的物理地图分辨率相对较低,包含约180个物理标记。只有Ae的三分之一。埃及埃及人的超重叠组合被分配到染色体上,但没有线性顺序和方向。我们最近发现,来自4龄幼虫成像盘的前中期染色体可以作为Ae物理定位的一个很好的来源。这是因为埃及埃及人的染色体较大,每张载玻片上的有丝分裂相染色体数目较多,以及单个染色体具有可重复的带型。本项目的具体目标是:1.建立Ae的细胞遗传学图谱。埃及伊蚊基于4龄幼虫成虫盘的带状前中期染色体。2.将约70%的基因组超重叠组合放置到其精确的染色体位置。这一开发项目将使Ae的细胞遗传学研究现代化。并极大地改善了目前支离破碎的基因组组装。我们相信,高分辨率物理地图的提供将加快与Ae正在进行的一些项目的进展。埃及,以及加强媒介生物学和昆虫基因组学,并将进一步刺激蚊子系统学、种群遗传学和昆虫比较基因组学的研究。 公共卫生相关性:埃及伊蚊是登革热病毒的主要全球传播媒介。登革热是全世界亚热带和热带地区的一种新的健康威胁。没有疫苗或药物治疗;因此,疾病预防在很大程度上是基于努力控制或避免接触受感染的蚊子。这项拟议的研究将开发新的基因组工具,将极大地提高现有Ae草案基因组序列组装的效用。埃及伊蚊,从而促进先进基因组技术的应用,以研究和开发新的登革热传播遗传控制策略。

项目成果

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Maria Sharakhova其他文献

Maria Sharakhova的其他文献

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{{ truncateString('Maria Sharakhova', 18)}}的其他基金

Haplotype-resolved genome assemblies and chromosomal rearrangements in arboviral vector Aedes albopictus
虫媒病毒载体白纹伊蚊中单倍型解析的基因组组装和染色体重排
  • 批准号:
    10573961
  • 财政年份:
    2022
  • 资助金额:
    $ 20.52万
  • 项目类别:
Chromosomal rearrangements in arboviral vector Aedes aegypti
虫媒病毒载体埃及伊蚊的染色体重排
  • 批准号:
    9974917
  • 财政年份:
    2020
  • 资助金额:
    $ 20.52万
  • 项目类别:
Chromosome scale genome assemblies for Culex pipiens mosquitoes
淡色库蚊的染色体规模基因组组装
  • 批准号:
    10302318
  • 财政年份:
    2020
  • 资助金额:
    $ 20.52万
  • 项目类别:
Improving the reference genome assembly for the dengue fever vector Aedes aegypti
改进登革热载体埃及伊蚊的参考基因组组装
  • 批准号:
    9017188
  • 财政年份:
    2016
  • 资助金额:
    $ 20.52万
  • 项目类别:
Chromosome-based genome assembly for Culex quinquefasciatus
基于染色体的致倦库蚊基因组组装
  • 批准号:
    8511874
  • 财政年份:
    2013
  • 资助金额:
    $ 20.52万
  • 项目类别:
Chromosome-based genome assembly for Culex quinquefasciatus
基于染色体的致倦库蚊基因组组装
  • 批准号:
    8604136
  • 财政年份:
    2013
  • 资助金额:
    $ 20.52万
  • 项目类别:
Physical mapping of Aedes aegypti genome
埃及伊蚊基因组的物理作图
  • 批准号:
    8137887
  • 财政年份:
    2010
  • 资助金额:
    $ 20.52万
  • 项目类别:
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