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Purchase of a Thermo Fisher Scientific LTQ Orbitrap XL Mass Spectrometer

购买 Thermo Fisher Scientific LTQ Orbitrap XL 质谱仪

基本信息

批准号：
7838622
负责人：
I JONATHAN AMSTER
金额：
$ 83.2万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-07 至 2012-08-06
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Thermo Fisher Scientific LTQ Orbitrap XL liquid chromatography-mass spectrometer for the University of Georgia. This instrument will be housed in the Proteomics and Mass Spectrometry (PAMS) Facility, which is a core facility that provides mass spectrometry service to the scientific community of the entire university. This instrument will provide liquid chromatography-tandem mass spectrometry (LC-MS/MS) capabilities that are not currently available in the PAMS facility, but that are required by a significant number of NIH-funded researchers at UGA. This instrument will support the research efforts of nine major users with fifteen NIH grants as well as 16 well-funded minor users, representing 11 different departments and four colleges across the university. The projects of the investigators include studies of human pathogens and parasites, insect vectors of human disease, fundamental investigations of the mechanisms of cancer, discovery of cancer biomarkers that can be used for early detection, neurochemistry, and basic studies of biology, all of which directly impact public health. The instrument will be used primarily for proteomic analysis, and to a lesser degree for lipidomic analysis. Protein analyses include identification, quantitation, and post-translational modification mapping. Capillary scale HPLC will be used on protein proteolytic digests, and analysis will include high resolution, accurate mass measurements and tandem mass spectrometry using collisionally induced dissociation and electron transfer dissociation. PUBLIC HEALTH RELEVANCE: The mass spectrometer that will be purchased with the funding of this proposal will provide analytical support for the research activities of nine major users who hold 15 NIH grants, as well as 16 minor users, most of whom are funded by NIH, or participate in NIH-funded research. The projects of the investigators include studies of human pathogens and parasites, insect vectors of human disease, fundamental investigations of the mechanisms of cancer, discovery of cancer biomarkers that can be used for early detection, and basic studies of biology, all of which directly impact public health. The funding of this proposal will impact job creation and maintenance in several tangible ways. First, the Vice President for Research of the University of Georgia will create and fund a position for a PhD level staff scientist in the Proteomics and Mass Spectrometry Facility (PAMS), should this proposal be funded (see attached letter of support). Furthermore, we anticipate that within a year or two, the income generated by this instrument will allow the PAMS facility to hire a technician. Thus, the funding of this grant will lead to the immediate creation of one job, and the future creation of a second job at the University of Georgia. The purchase of this mass spectrometer will impact jobs with the instrument vendor, as well. We have selected an instrument from Thermo Fisher Scientific, which is based in the United States, with headquarters in Waltham, Massachusetts. They employ approximately 20,000 people in more than 200 facilities across the country. Of these facilities, approximately 100 are used for manufacturing, including major sites in San Jose and Fremont, California; Asheville, North Carolina; Pittsburgh, Pennsylvania; Sugar Land, Texas; Madison, Wisconsin; Franklin, Massachusetts and Marietta, Ohio. About 80 percent of their US workforce is engaged in manufacturing, sales and service, engineering, and scientific research and development jobs. The environmental impact of the instrument is favorable. On the manufacturing side, Thermo Fisher Scientific is committed to being environmentally responsible in the way they run their company and in the products they manufacture for their customers. Their facilities worldwide operate under a program that generally follows the ISO 14001 environmental management standard, with additional requirements outlined in their internal energy and waste management policies. With regards to the environmental impact of the operation of the requested LC-mass spectrometer, we have specifically requested a capillary HPLC system with this instrument. This utilizes organic solvents at flow rates of a few ¿L/min, 1000 times lower than conventional liquid chromatography which uses mL/min flow rates. This ensures that this instrument will generate minimal (less than 5 mL per day) of chemical waste in its day-to-day operation.

描述（由申请人提供）：本提案要求为格鲁吉亚大学购买Thermo Fisher Scientific LTQ Orbitrap XL液相色谱-质谱仪提供资金。该仪器将被安置在蛋白质组学和质谱（PAMS）设施，这是一个核心设施，为整个大学的科学界提供质谱服务。该仪器将提供液相色谱-串联质谱（LC-MS/MS）功能，目前在PAMS设施中不可用，但需要大量NIH资助的研究人员在UGA。该工具将支持9个主要用户的研究工作，其中包括15个NIH赠款以及16个资金充足的次要用户，代表整个大学的11个不同部门和4个学院。研究人员的项目包括人类病原体和寄生虫的研究，人类疾病的昆虫媒介，癌症机制的基础研究，可用于早期检测的癌症生物标志物的发现，神经化学和生物学的基础研究，所有这些都直接影响公共卫生。该仪器将主要用于蛋白质组学分析，并在较小程度上用于脂质组学分析。蛋白质分析包括鉴定、定量和翻译后修饰图谱。毛细管规模HPLC将用于蛋白质蛋白水解酶，分析将包括高分辨率、准确的质量测量和使用碰撞诱导解离和电子转移解离的串联质谱。公共卫生相关性：将使用本提案的资金购买的质谱仪将为持有15项NIH赠款的9个主要用户以及16个次要用户的研究活动提供分析支持，其中大多数用户由NIH资助或参与NIH资助的研究。研究人员的项目包括人类病原体和寄生虫的研究，人类疾病的昆虫媒介，癌症机制的基础研究，发现可用于早期检测的癌症生物标志物，以及生物学的基础研究，所有这些都直接影响公共卫生。这项提案的资金将以几种切实的方式影响就业机会的创造和维持。首先，格鲁吉亚大学研究副校长将为蛋白质组学和质谱设施（PAMS）的博士级工作人员科学家创建并资助一个职位，如果该提案得到资助（见所附的支持信）。此外，我们预计在一两年内，该仪器产生的收入将使PAMS设施能够雇用一名技术人员。因此，这笔赠款的资金将导致立即创造一个就业机会，并在格鲁吉亚大学的第二个就业机会的未来创造。购买这台质谱仪也会影响仪器供应商的工作。我们选择了Thermo Fisher Scientific公司的仪器，该公司总部设在美国，位于马萨诸塞州沃尔瑟姆。他们在全国200多个工厂雇用了大约20，000人。其中，约100家工厂用于生产，包括加州的圣何塞和弗里蒙特、北卡罗来纳州的阿什维尔、宾夕法尼亚州的匹兹堡、德克萨斯州的糖地、威斯康星州的麦迪逊、富兰克林、马萨诸塞州和俄亥俄州的玛丽埃塔的主要工厂。大约80%的美国劳动力从事制造、销售和服务、工程以及科学研究和开发工作。仪器的环境影响是有利的。在制造方面，赛默飞世尔科技致力于在经营公司的方式和为客户生产的产品中对环境负责。我们在全球各地的设施都按照ISO 14001环境管理标准的计划运营，并在内部能源和废物管理政策中列出了其他要求。关于所要求的LC-质谱仪操作的环境影响，我们特别要求使用该仪器的毛细管HPLC系统。这利用有机溶剂，流速为几升/分钟，比使用mL/分钟流速的常规液相色谱低1000倍。这确保了该仪器在日常操作中产生的化学废物最少（每天少于5 mL）。

项目成果

期刊论文数量（39）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.

DOI：
10.1016/j.chroma.2018.02.052
发表时间：
2018-04-13
期刊：
Journal of chromatography. A
影响因子：
0
作者：
Sanderson P;Stickney M;Leach FE 3rd;Xia Q;Yu Y;Zhang F;Linhardt RJ;Amster IJ
通讯作者：
Amster IJ

Widespread genome reorganization of an obligate virus mutualist.

专性病毒共同主义的广泛基因组重组。