An Automated Platform for the CE-MS Analysis of Glycosaminoglycans
用于糖胺聚糖 CE-MS 分析的自动化平台
基本信息
- 批准号:9753175
- 负责人:
- 金额:$ 29.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-01 至 2021-07-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAnabolismAnimalsAutomationBindingBinding ProteinsBiochemical PathwayBiologicalBiological AssayBiological ProcessBiologyBiopolymersCapillary ElectrophoresisCarbohydratesCellsComplexComplex MixturesDataDeacetylationDevelopmentDisaccharidesDiseaseEnvironmentEnzymesFundingGAG GeneGenerationsGlucuronic AcidsGlycosaminoglycansGoalsGolgi ApparatusHealthHexosaminesHumanInfectionIonsLaboratoriesLengthMammalian CellMass Spectrum AnalysisMethodsModificationNamesNormal RangeOrganismOutcomePathologic ProcessesPatternPeptidesPhysiologicalPlayPolysaccharidesProceduresProcessProtein RegionProteinsReagentRegulationResearch PersonnelRoleSamplingSeriesSignal TransductionSiteSite-Directed MutagenesisSpecificityStretchingStructureSurfaceSystemTechnologyTestingUnspecified or Sulfate Ion SulfatesUronic AcidsViruscell motilitycopolymerepimerizationinnovationinstrumentinterestmass spectrometermethod developmentpolysulfated glycosaminoglycansoftware developmentsulfationtandem mass spectrometrytool
项目摘要
Project Summary/Abstract
This project will develop an automated capillary electrophoresis-tandem mass spectrometry platform for the
structural analysis of mixtures of glycosaminoglycan oligomers that are isolated in protein binding assays. This
class of carbohydrates can be found on the surface of all animal cells, and play a key role in many important
biological processes. Glycosaminoglycans are linear, polysulfated copolymers, typically with a repeating
disaccharide unit composed of an N-acetyl-hexosamine and glucuronic acid, and can be more than 200 repeat
units long. Modifications can occur anywhere along the chain, and these include O-sulfation, N-
deacetylation/N-sulfation, and epimerization of the uronic acid. These are a particularly challenging class of
compounds to characterize due to their heterogeneous structures, an outcome of their non-template
biosynthesis. Nevertheless, within the long glycan chains are short regions of defined modification that target
specific proteins with remarkable specificity. These glycan-protein interactions are the basis for the biological
activity of the glycosaminoglycan. There is a critical need for tools to find these organized regions with the long
glycan chain, and to determine their pattern of modification. A few specialized laboratories have developed
mass spectrometry methods for the structural analysis of glycosaminoglycans, but these have not found their
way into biological laboratories at large. The goal of this project is to produce a platform with a high degree of
automation so that it can be deployed in the laboratories of biology researchers. The accessibility of these
advanced mass spectrometry methods will result from innovations in hardware, software, and methods
development.
项目总结/摘要
本项目将开发一个自动化的毛细管电泳-串联质谱分析平台,
在蛋白质结合测定中分离的糖胺聚糖低聚物的混合物的结构分析。
一类碳水化合物可以在所有动物细胞的表面上找到,并在许多重要的生物学过程中发挥关键作用。
糖胺聚糖是线性的多硫酸化共聚物,通常具有重复的
由N-乙酰基-N-己糖胺和葡萄糖醛酸组成的二糖单元,可以有超过200个重复
修饰可以发生在沿着链的任何地方,这些修饰包括O-硫代硫酸化、N-硫代硫酸化和N-硫代硫酸化。
糖醛酸的脱乙酰化/N-乙酰基硫酸化和差向异构化。
由于其结构的异质性,化合物难以表征,
然而,在长聚糖链内是靶向生物合成的限定修饰的短区域。
具有显著特异性的特异性蛋白质。这些聚糖-蛋白质相互作用是生物学的基础。
迫切需要工具来找到这些具有长链糖胺聚糖活性的有组织区域。
聚糖链,并确定其修饰模式。一些专门的实验室已经开发了
用于糖胺聚糖结构分析的质谱方法,但这些方法尚未发现它们的
进入生物实验室的方式。该项目的目标是产生一个平台,
自动化,以便它可以部署在生物研究人员的实验室。这些
先进的质谱分析方法将来自于硬件、软件和方法的创新
发展
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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I JONATHAN AMSTER其他文献
I JONATHAN AMSTER的其他文献
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{{ truncateString('I JONATHAN AMSTER', 18)}}的其他基金
T32 Predoctoral training grant in Glycosciences
T32 糖科学博士前培训补助金
- 批准号:
10410757 - 财政年份:2022
- 资助金额:
$ 29.47万 - 项目类别:
T32 Predoctoral training grant in Glycosciences
T32 糖科学博士前培训补助金
- 批准号:
10650310 - 财政年份:2022
- 资助金额:
$ 29.47万 - 项目类别:
An Automated Platform for the CE-MS Analysis of Glycosaminoglycans
用于糖胺聚糖 CE-MS 分析的自动化平台
- 批准号:
10005264 - 财政年份:2018
- 资助金额:
$ 29.47万 - 项目类别:
Rapid Sequencing of Sulfated Glycans By CE-MS/MS
通过 CE-MS/MS 对硫酸化聚糖进行快速测序
- 批准号:
9336350 - 财政年份:2016
- 资助金额:
$ 29.47万 - 项目类别:
Purchase of a Thermo Fisher Scientific LTQ Orbitrap XL Mass Spectrometer
购买 Thermo Fisher Scientific LTQ Orbitrap XL 质谱仪
- 批准号:
7838622 - 财政年份:2011
- 资助金额:
$ 29.47万 - 项目类别:
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