UPLC-MS/MS to Support Preclinical and Clinical Antiretroviral Pharmacology Studie

描述（由申请人提供）：本提案的目的是为北卡罗来纳大学教堂山分校（UNC-CH）艾滋病研究中心（CFAR）建立的临床药理学和分析化学核心（CPACC）提供第二台专用UPLC-MS/MS仪器。CPACC有11年的历史，提供了一个最先进的中央clia认证单位，以维持北卡罗来纳大学-卫生院、国际家庭健康研究所和三角研究所的合作机构以及校外CFARs的艾滋病毒/艾滋病相关临床和基础科学研究的药理学和分析研究。CPACC的成功最好的例证是，在2005年UNC CFAR为期五年的续期期间，通过分析创新支持国内和国际研究人员，在同行评审中获得了出色的分数。从那时起，CPACC不断发展壮大，现在每年帮助50多名用户。CPACC已经在2007年购买的Agilent 6410A三重四极杆LC-MS/MS系统上证明了成功。这一工具正在全时用于网络艾滋病毒预防研究。由于工作量大，并且缺乏额外的仪器，其他需要质谱/质谱技术的关键项目（例如用于艾滋病毒预防和治疗的人源化小鼠模型药理学，生殖道抗逆转录病毒药物的细胞药理学）要么被搁置，要么被拒绝。第二台UPLC-MS/MS仪器的增加将支持美国国立卫生研究院资助的CFAR研究人员确保他们的项目能够快速有效地完成，将为研究三角地区的人们创造就业机会，将为当地企业提供资金，并为UNC-CH系统内的长期经济增长奠定基础。成本回收费用计划保证了该工具的财务可行性。Angela DM Kashuba博士（CPACC主任）担任该提案的PI，并将负责仪器维护和有效使用的所有方面，以产生新的生物分析创新。该仪器将与CPACC的其他分析仪器一起安置在药学院。

DESCRIPTION (provided by applicant): The purpose of this proposal is to provide a second dedicated UPLC-MS/MS instrument to the established Clinical Pharmacology and Analytical Chemistry Core (CPACC) of the University of North Carolina at Chapel Hill (UNC-CH) Center for AIDS Research (CFAR). The CPACC has an 11-year record of providing a state-of- the-art centralized CLIA-certified unit to sustain the pharmacology and analytical studies of HIV/AIDS related clinical and basic science research at UNC-CH, the collaborating institutions of Family Health International and Research Triangle Institute, and to extramural CFARs. The success of the CPACC is best exemplified by the outstanding score it received from peer reviewers during the UNC CFAR's five-year renewal in 2005 for support of domestic and international investigators through analytical innovation. Since that time, the CPACC has continued to grow, and now assists more than 50 users per year. The CPACC has demonstrated success on the Agilent 6410A Triple Quadrupole LC-MS/MS System purchased in 2007. This instrument is being used full-time for network HIV prevention studies. Because of this workload, and due to lack of additional instrumentation, other key projects requiring MS/MS technology (eg humanized mouse model pharmacology for HIV prevention and cure, cellular pharmacology of antiretrovirals in the genital tract) are either on hold, or are being turned down. The addition of a second UPLC-MS/MS instrument will support NIH-funded CFAR investigators to ensure that their projects can be completed quickly and efficiently, will create job opportunities for those in the Research Triangle Area, will provide capital to local businesses, and build a foundation for longer-term economic growth within the UNC-CH system. A cost recovery fee plan guarantees the financial viability for the instrument. Dr. Angela DM Kashuba (Director of the CPACC) serves as PI of the proposal and will be responsible for all aspects of instrument maintenance and efficient usage in generating new bioanalytical innovations. The instrument will be housed in the School of Pharmacy with the other analytical instruments of the CPACC.