Nikon A1R Confocal Laser Scanning Microscope

尼康 A1R 共焦激光扫描显微镜

• 批准号: 8051996
• 负责人: MICHAEL A. MANCINI
• 金额: $ 43.14万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051996
• 关键词: Aging; Computer software; Event; Gases; Housing; Image; Lasers; Life; Location; Medicine; Microscope; Microscopy; Positioning Attribute; Research Personnel; Resolution; Resources; Scanning; Series; Specimen; Speed; System; Temperature; Three-Dimensional Imaging; Time; Training; United States National Institutes of Health; assay development; college; flexibility; imaging detector; user friendly software

DESCRIPTION (provided by applicant): This request from a long-standing Baylor College of Medicine (BCM) microscopy core is for a new, fully-automated, state-of-the-art Nikon A1 laser scanning confocal microscope to replace an aging confocal system. The requirements include being able to provide: (i) high resolution imaging at wavelengths covering the near UV, visible and near IR spectral range using both conventional and spectral imaging detectors; (ii) long-term time-lapse imaging with automatic focus and controlled gas/temperature; (iii) high-speed confocal imaging to track dynamic events in X and Y, and perform rapid Z-series acquisition at sequential time points (4D imaging); (iv) combining fast, accurate and repeatable autofocus and XYZ positioning control to allow 2D and 3D imaging at multiple locations in single to 96/384-well plate formats for high-speed, high- resolution imaging of fixed or live specimens; and, (v) enough flexibility and reliability (multiple wavelengths, conventional and spectral, and multiple locations) for true multidimensional confocal imaging controlled by user-friendly software. Long-term in-house demonstration of the Nikon A-1 has demonstrated its ability fulfill these requirements and those of the diverse experimental plans of a well-defined group of NIH-supported investigators. The system will be housed and managed by the Integrated Microscopy Core (IMC), the only fully-open-access imaging facility at BCM, and will synergize with and extend current automated microscopy resources, including hardware/software training and assay development support.

描述(由申请人提供)：贝勒医学院(BCM)长期使用的显微镜核心提出的这一请求是为了使用一种新型的、全自动化的、最先进的尼康A1激光扫描共聚焦显微镜，以取代老化的共聚焦系统。这些要求包括能够提供：(1)使用常规和光谱成像探测器在覆盖近紫外、可见光和近红外光谱范围的波长上进行高分辨率成像；(2)具有自动聚焦和控制气体/温度的长期延时成像；(3)高速共焦成像，以跟踪X和Y的动态事件，并在连续的时间点执行快速Z系列采集(4D成像)；(Iv)结合快速、准确和可重复的自动对焦和XYZ定位控制，可在多个位置以单一至96/384孔板格式进行2D和3D成像，以对固定或活标本进行高速、高分辨率成像；以及，(V)有足够的灵活性和可靠性(多波长、常规和光谱及多位置)，可由用户友好的软件控制进行真正的多维共焦成像。尼康A-1的长期内部演示表明，它有能力满足这些要求，以及由NIH支持的一组明确定义的研究人员的各种实验计划的要求。该系统将由集成显微镜核心(IMC)容纳和管理，这是BCM唯一的完全开放的成像设施，并将与现有的自动显微镜资源协同工作并扩展，包括硬件/软件培训和分析开发支持。