CORE D - INTEGRATED MICROSCOPY CORE

集成显微镜核心一直是U54生殖生物学中心的重要资源 从35年前成立以来。核心，使常规和复杂的光学显微镜(LM)和 电子显微镜(EM)在上一次筹资期间大力支持了所有四个项目，并继续 作为当前提案的一个不可或缺的支助部分。 该中心为广泛的生物项目提供了LM和EM资源、培训和服务。 这些包括长期存在的组织学和超微结构标本的一般成像，以及 年的发展提供了越来越多的基于图像的集成方法，这些方法将 生物化学、细胞生物学、分子和遗传学研究(例如，相互作用、表达水平、免疫学、 表位标记、报告基因研究等)。最近的技术转变是增加了 实现自动化并获得高度定量的功能和细胞学测量 通过安装高通量显微镜(HTM)设备。实际上，他们的能力 集成显微镜核心现在不仅提供常规和高级成像资源，而且是 日益将高通量系统生物学作为研究和开发工具推向前沿 为U54项目带来新技术。 HTM对于U54应用中的研究人员来说将是至关重要的。本次U54更新的研究重点 应用是研究共调节因子、转录因子、细胞信号和微小RNA在 小鼠和人子宫内膜和卵巢功能的调节。所有这些方法都将利用 影像作为评估子宫内膜生物学的工具。执行这些项目的目标将需要 核心将成为一个不断发展的工具。

The Integrated Microscopy Core has been an important resource for this U54 Center for Reproductive Biology from its inception over 35 years ago. The Core, enabling routine and sophisticated light microscopy (LM) and electron microscopy (EM), has significantly supported all four Projects in the last funding period, and continues to be an integral support component of the current proposal. The Core has provided LM and EM resources, training and services for a wide range of biological projects. These include the longstanding general imaging of histological and ultrastructural specimens, and over the years has evolved to provide an increasing number of integrated image-based approaches that bring together biochemistry, cell biology, molecular and genetic studies (e.g., interaction, expression levels, immunological, epitope-tagging, reporter gene studies, etc). The most recent technological shift has been to increased implementation of automation and highly quantitative functional and cytological measurements obtained through the installation of high throughput microscopy (HTM) equipment. Effectively, the ability of the Integrated Microscopy Core now provides not only the routine and advanced imaging resource, but is increasingly bringing to the forefront high throughput systems biology as a Research & Development tool to bring new technologies to the U54 Projects. HTM will be critical for the investigators in this U54 application. The focus of the research in this U54 renewal application is the investigation of the role of co-regulators, transcription factors, cell signaling and micro RNA in the regulation of endometrial and ovarian function in the mouse and human. All these approaches will utilize imaging as a tool to evaluate endometrial biology. The execution of the aims of these projects will require the core to be an evolving tool.