INTEGRATED MICROSCOPY

综合显微镜共享资源（IMSR）是为Dan L. Duncan癌症中心（DLDCC）研究人员提供的最先进的设备、培训和咨询成像资源。其使命是为DLDCC和其他BCM研究人员提供全套光学和电子显微镜（LM， EM）和分析开发资源。IMSR的工作人员在基于图像的调查的各个方面都受过良好的培训，并与IMSR领导的研究项目合作，始终站在新实验方法的最前沿。IMSR目前支持使用手动和自动高通量显微镜（HTM）对活细胞和固定细胞进行基于图像的研究，也支持通过透射电镜进行超微结构研究。LM资源包括新的尼康AIR多光谱显微镜和蔡司LSM 510 META共聚焦显微镜。新的DeltaVision“核心”反卷积范围和彻底升级的原始DeltaVision现在与蔡司AxioPlan2/CCD工作站一起使用。尼康和DeltaVison核心仪器是完全活细胞和FRET能力，两者都适用于多孔板的使用。标准

The Integrated Microscopy Shared Resource (IMSR) is a state-of-the-art equipment, training, and consultation imaging resource resource for scores of Dan L. Duncan Cancer Center (DLDCC) researchers. Its mission is to provide a full suite of light and electron microscopy (LM, EM), and assay development resources for DLDCC and other BCM investigators. The IMSR staff is well-trained in all aspects of image based investigation and stays at the forefront of new experimental approaches in collaboration with the IMSR leader's research program. The IMSR currently supports image-based studies for both live and fixed cells using manual and automated high throughput microscopy (HTM), and also for ultrastructural studies via transmission EM. The LM resources consist of a new Nikon AIR multispectral and Zeiss LSM 510 META confocal microscopes. A new DeltaVision "Core" deconvolution scope and a thoroughly-upgraded original DeltaVision are now in use along with a Zeiss AxioPlan2/CCD workstation. The Nikon and DeltaVison Core instruments are fully live cell and FRET capable, and both are suitable for multiwell plate usage. Standard HTM capabilities are based upon the two fully-automated Beckman-Coulter IC-100 systems with robotic platforms for fluid handling and plate loading. High content analysis (HCA) support is based upon the fully customizable Pipeline Pilot image analysis platform. HTM and automated image processing are synergistic with primary screening of monoclonal antibodies (Proteomics Shared Resource), and RNAi libraries (RNAi Shared Resource) for high speed pathway analysis in single cell assays. The Hitachi H-7500 transmission digital EM supports ultrastructural studies. IMSR staff include two imaging technologists and a registered electron microscopy technician. IMSR goals are to facilitate and support DLDCC researchers through collaboration on experimental design, user training on all instrumentation and software, and as needed, direct image acquisition or processing by IMSR staff, and assistance in data interpretation and presentation. The IMSR will continue to expand to meet DLDCC needs through equipment grants and vendor arrangements in order to provide Cancer Center investigators with basic and leading-edge imaging infrastructure and expertise.