INTEGRATED MICROSCOPY

集成显微镜

• 批准号: 8180984
• 负责人: MICHAEL A. MANCINI
• 金额: $ 9.21万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-17 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180984
• 关键词: Address; Arts; Automation; Biological Assay; Biometry; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cells; Cellular biology; Collaborations; Collection; Complex; Computer software; Consultations; Data; Development; Electron Microscopy; Equipment; Experimental Designs; Experimental Models; Faculty; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Generic Drugs; Goals; Grant; Health Services Accessibility; Housing; Human Resources; Image; Image Analysis; Informatics; Institution; Investigation; Laboratories; Laboratory Research; Laser Scanning Confocal Microscopy; Libraries; Life; Light; Liquid substance; Location; Malignant Neoplasms; Manuals; Medical Research; Medicine; Microscope; Microscopy; Mission; Molecular and Cellular Biology; Monoclonal Antibodies; Optics; Pathway Analysis; Process; Proteomics; Proteomics Shared Resource; RNA Interference; Recording of previous events; Reproductive Biology; Research; Research Infrastructure; Research Personnel; Resolution; Resource Sharing; Resources; Robotics; Scientist; Screening procedure; Services; Speed; System; Systems Biology; Techniques; Technology; Tissues; Training; Transmission Electron Microscopy; Vendor; Work; anticancer research; assay development; base; cell fixing; college; cost; digital; digital imaging; genome-wide; high standard; high throughput screening; image processing; instrument; instrumentation; light microscopy; meetings; member; operation; programs; repository; tool; transmission process

The Integrated Microscopy Shared Resource (IMSR) is a state-of-the-art equipment, training, and consultation imaging resource resource for scores of Dan L. Duncan Cancer Center (DLDCC) researchers. Its mission is to provide a full suite of light and electron microscopy (LM, EM), and assay development resources for DLDCC and other BCM investigators. The IMSR staff is well-trained in all aspects of image based investigation and stays at the forefront of new experimental approaches in collaboration with the IMSR leader's research program. The IMSR currently supports image-based studies for both live and fixed cells using manual and automated high throughput microscopy (HTM), and also for ultrastructural studies via transmission EM. The LM resources consist of a new Nikon AIR multispectral and Zeiss LSM 510 META confocal microscopes. A new DeltaVision "Core" deconvolution scope and a thoroughly-upgraded original DeltaVision are now in use along with a Zeiss AxioPlan2/CCD workstation. The Nikon and DeltaVison Core instruments are fully live cell and FRET capable, and both are suitable for multiwell plate usage. Standard HTM capabilities are based upon the two fully-automated Beckman-Coulter IC-100 systems with robotic platforms for fluid handling and plate loading. High content analysis (HCA) support is based upon the fully customizable Pipeline Pilot image analysis platform. HTM and automated image processing are synergistic with primary screening of monoclonal antibodies (Proteomics Shared Resource), and RNAi libraries (RNAi Shared Resource) for high speed pathway analysis in single cell assays. The Hitachi H-7500 transmission digital EM supports ultrastructural studies. IMSR staff include two imaging technologists and a registered electron microscopy technician. IMSR goals are to facilitate and support DLDCC researchers through collaboration on experimental design, user training on all instrumentation and software, and as needed, direct image acquisition or processing by IMSR staff, and assistance in data interpretation and presentation. The IMSR will continue to expand to meet DLDCC needs through equipment grants and vendor arrangements in order to provide Cancer Center investigators with basic and leading-edge imaging infrastructure and expertise.

综合显微镜共享资源(IMSR)是为丹·L·邓肯癌症中心(DLDCC)的数十名研究人员提供的最先进的设备、培训和咨询成像资源。它的使命是为DLDCC和其他BCM调查人员提供全套光学和电子显微镜(LM，EM)和分析开发资源。IMSR的工作人员在基于图像的调查的所有方面都接受了良好的培训，并与IMSR领导者的研究计划合作，站在了新实验方法的前沿。IMSR目前支持使用手动和自动高通量显微镜(HTM)对活细胞和固定细胞进行基于图像的研究，也支持通过透射EM进行超微结构研究。LM资源包括一台新的尼康空气多光谱显微镜和蔡司LSM 510元共焦显微镜。新的DeltaVision“核心”反卷积范围和彻底升级的原始DeltaVision现在与蔡司AxioPlan2/CCD工作站一起使用。尼康和DeltaVison核心仪器完全支持带电电池和FRET，两者都适用于多孔板。标准 HTM的能力基于两个全自动Beckman-Coulter IC-100系统，该系统带有用于流体处理和板材装载的机器人平台。高内容分析(HCA)支持基于完全可定制的管道试点图像分析平台。HTM和自动图像处理与单抗(蛋白质组学共享资源)和RNAi文库(RNAi共享资源)的初步筛选协同作用，用于单细胞分析中的高速通路分析。日立H-7500变速箱数字EM支持超微结构研究。IMSR的工作人员包括两名成像技术员和一名注册电子显微镜技术员。IMSR的目标是通过在实验设计方面的合作、对所有仪器和软件的用户培训，并在需要时由IMSR工作人员直接获取或处理图像，以及在数据解释和介绍方面提供协助，来便利和支持DLDCC研究人员。IMSR将通过设备赠款和供应商安排继续扩大，以满足DLDCC的需求，以便为癌症中心的研究人员提供基本和尖端的成像基础设施和专业知识。