INTEGRATED MICROSCOPY

集成显微镜

• 批准号: 8180984
• 负责人: MICHAEL A. MANCINI
• 金额: $ 9.21万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-17 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180984
• 关键词: Address; Arts; Automation; Biological Assay; Biometry; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cells; Cellular biology; Collaborations; Collection; Complex; Computer software; Consultations; Data; Development; Electron Microscopy; Equipment; Experimental Designs; Experimental Models; Faculty; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Generic Drugs; Goals; Grant; Health Services Accessibility; Housing; Human Resources; Image; Image Analysis; Informatics; Institution; Investigation; Laboratories; Laboratory Research; Laser Scanning Confocal Microscopy; Libraries; Life; Light; Liquid substance; Location; Malignant Neoplasms; Manuals; Medical Research; Medicine; Microscope; Microscopy; Mission; Molecular and Cellular Biology; Monoclonal Antibodies; Optics; Pathway Analysis; Process; Proteomics; Proteomics Shared Resource; RNA Interference; Recording of previous events; Reproductive Biology; Research; Research Infrastructure; Research Personnel; Resolution; Resource Sharing; Resources; Robotics; Scientist; Screening procedure; Services; Speed; System; Systems Biology; Techniques; Technology; Tissues; Training; Transmission Electron Microscopy; Vendor; Work; anticancer research; assay development; base; cell fixing; college; cost; digital; digital imaging; genome-wide; high standard; high throughput screening; image processing; instrument; instrumentation; light microscopy; meetings; member; operation; programs; repository; tool; transmission process

The Integrated Microscopy Shared Resource (IMSR) is a state-of-the-art equipment, training, and consultation imaging resource resource for scores of Dan L. Duncan Cancer Center (DLDCC) researchers. Its mission is to provide a full suite of light and electron microscopy (LM, EM), and assay development resources for DLDCC and other BCM investigators. The IMSR staff is well-trained in all aspects of image based investigation and stays at the forefront of new experimental approaches in collaboration with the IMSR leader's research program. The IMSR currently supports image-based studies for both live and fixed cells using manual and automated high throughput microscopy (HTM), and also for ultrastructural studies via transmission EM. The LM resources consist of a new Nikon AIR multispectral and Zeiss LSM 510 META confocal microscopes. A new DeltaVision "Core" deconvolution scope and a thoroughly-upgraded original DeltaVision are now in use along with a Zeiss AxioPlan2/CCD workstation. The Nikon and DeltaVison Core instruments are fully live cell and FRET capable, and both are suitable for multiwell plate usage. Standard HTM capabilities are based upon the two fully-automated Beckman-Coulter IC-100 systems with robotic platforms for fluid handling and plate loading. High content analysis (HCA) support is based upon the fully customizable Pipeline Pilot image analysis platform. HTM and automated image processing are synergistic with primary screening of monoclonal antibodies (Proteomics Shared Resource), and RNAi libraries (RNAi Shared Resource) for high speed pathway analysis in single cell assays. The Hitachi H-7500 transmission digital EM supports ultrastructural studies. IMSR staff include two imaging technologists and a registered electron microscopy technician. IMSR goals are to facilitate and support DLDCC researchers through collaboration on experimental design, user training on all instrumentation and software, and as needed, direct image acquisition or processing by IMSR staff, and assistance in data interpretation and presentation. The IMSR will continue to expand to meet DLDCC needs through equipment grants and vendor arrangements in order to provide Cancer Center investigators with basic and leading-edge imaging infrastructure and expertise.

综合显微镜共享资源（IMSR）是一个国家的最先进的设备，培训和咨询成像资源的丹L。邓肯癌症中心（DLDCC）的研究人员。 它的使命是为DLDCC和其他研究人员提供全套的光学和电子显微镜（LM，EM）以及分析开发资源。IMSR的工作人员在基于图像的调查的各个方面都受过良好的培训，并与IMSR领导人的研究计划合作，走在新实验方法的最前沿。IMSR目前支持使用手动和自动高通量显微镜（HTM）对活细胞和固定细胞进行基于图像的研究，以及通过透射电镜进行超微结构研究。LM资源包括一个新的尼康AIR多光谱和蔡司LSM 510 Meta共焦显微镜。一个新的DeltaVision“核心”反卷积范围和一个彻底升级的原始DeltaVision现在沿着使用蔡司AxioPlan 2/CCD工作站。Nikon和DeltaVison Core仪器具有完全的活细胞和FRET能力，两者都适用于多孔板使用。标准 HTM能力基于两个全自动Beckman-Coulter IC-100系统，该系统具有用于流体处理和板加载的机器人平台。高含量分析（HCA）支持基于完全可定制的Pipeline Pilot图像分析平台。HTM和自动化图像处理与单克隆抗体（Proteomics Shared Resource）和RNAi文库（RNAi Shared Resource）的初级筛选具有协同作用，用于单细胞测定中的高速途径分析。日立H-7500传输数字EM支持超微结构研究。IMSR工作人员包括两名成像技术员和一名注册电子显微镜技术员。IMSR的目标是通过在实验设计方面的合作，所有仪器和软件的用户培训，以及根据需要，IMSR工作人员的直接图像采集或处理，以及数据解释和演示方面的协助，促进和支持DLDCC研究人员。 IMSR将通过设备赠款和供应商安排继续扩大以满足DLDCC的需求，以便为癌症中心研究人员提供基本和领先的成像基础设施和专业知识。