OMX Super Resolution Microscope

OMX 超分辨率显微镜

• 批准号: 8826339
• 负责人: MICHAEL A. MANCINI
• 金额: $ 59.48万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826339
• 关键词: Biology; Cells; Computer software; Electron Microscopy; Electrons; Event; Fluorochrome; Housing; Image; Image Analysis; Imaging Device; Laboratories; Lateral; Life; Light; Lighting; Liquid substance; Medicine; Microscope; Microscopy; Preparation; Resolution; Resources; Sampling; Scanning Probe Microscopes; Speed; System; Techniques; Three-Dimensional Image; Three-Dimensional Imaging; Time; Training; Work; assay development; college; diffraction of light; fluorescence imaging; fluorescence microscope; instrumentation; light microscopy; optical imaging; three dimensional structure

 DESCRIPTION (provided by applicant): The present application is to request a high speed multicolor 3D structured illumination super-resolution fluorescence microscope (SIM) for the Integrated Microscopy Core (IMC), a long standing shared instrumentation facility at the Baylor College of Medicine. The requested OMX" system with the Blaze" SIM- module from Applied Precision is the first commercially available super-resolution system capable of acquiring 3D images with lateral and axial resolutions at least two times smaller than the classical limit imposed by the diffraction of light, at speeds compatible with recording fast dynamic events in live cells and at multiple wavelengths by the use of multiple high resolution-high sensitivity CMOS cameras. The system allows 3D imaging at a level of detail not possible with confocal microscopes or wide field 3D deconvolution systems. In contrast to other high resolution imaging tools commonly used in biology, such as electron and atomic force microscopes, specialized near-field probe scanning microscopes and other newly developed and commercially available far-field super resolution techniques such as STED, PALM and STORM, the OMX 3D SIM system is able to achieve multicolor fluorescence imaging and is also built for image acquisition speeds and low levels of light exposure compatible with live cell studies. Collectively, the OMX overcomes spatial and temporal limitations presented by other microscopy systems. In addition, the OMX: a) works with conventional fluorochromes, b) does not present the geometrical limitations to the sample present in other high resolution systems, c) does not require special mounting and preparation techniques, and d) is not more complicated to operate than other commercially available microscopy systems already in use in the IMC. The OMX is the fastest 3D SIM system commercially available and is the only one that takes advantage of multiple liquid cooled sCMOS cameras (up to 4) for multi-wavelength imaging. The OMX will be housed in completely new renovated spaces for the IMC and adjacent PI's laboratory. An OMX addition to the IMC, an open access optical imaging facility at Baylor supporting light and electron microscopy, will synergize with and extend current state of the art imaging and image analysis resources, including hardware/software training and assay development support.

 描述(申请人提供)：本申请是为集成显微镜核心(IMC)请求高速多色3D结构照明超分辨率荧光显微镜(SIM)，IMC是贝勒医学院的长期共享仪器设施。应用精密公司要求的OMX“带火焰的系统”SIM模块是第一个商用的超分辨率系统，能够采集横向和轴向分辨率至少比经典的光衍射极限小两倍的3D图像，速度与在活细胞中记录快速动态事件的速度兼容，并通过使用多个高分辨率-高灵敏度CMOS相机以多个波长采集图像。该系统允许以共焦显微镜或广域3D去卷积系统所不能达到的细节水平进行3D成像。与生物学中常用的其他高分辨率成像工具不同，如电子显微镜和原子力显微镜、专门的近场探针扫描显微镜和其他新开发的、可商业化的远场超分辨率技术，如STED、Palm和STORM，OMX 3D SIM系统能够实现多色荧光成像，并可实现与活细胞研究兼容的图像采集速度和低水平光曝光。总的来说，OMX克服了其他显微镜系统带来的空间和时间限制。此外，OMX：a)使用传统的荧光显色剂，b)不存在其他高分辨率系统中存在的样品的几何限制，c)不需要特殊的安装和制备技术，以及d)操作不比已经在IMC中使用的其他商业可获得的显微系统更复杂。OMX是市面上可用的速度最快的3D SIM系统，也是唯一一款利用多个液体冷却sCMOS机(最多4个)进行多波长成像的系统。OMX将被安置在IMC和毗邻的PI实验室的全新翻新空间中。IMC是贝勒的一家开放式光学成像设施，支持光学和电子显微镜，在IMC上增加一个OMX将与当前最先进的成像和图像分析资源协同工作，并扩展这些资源，包括硬件/软件培训和分析开发支持。