OMX Super Resolution Microscope

OMX 超分辨率显微镜

• 批准号: 8826339
• 负责人: MICHAEL A. MANCINI
• 金额: $ 59.48万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826339
• 关键词: Biology; Cells; Computer software; Electron Microscopy; Electrons; Event; Fluorochrome; Housing; Image; Image Analysis; Imaging Device; Laboratories; Lateral; Life; Light; Lighting; Liquid substance; Medicine; Microscope; Microscopy; Preparation; Resolution; Resources; Sampling; Scanning Probe Microscopes; Speed; System; Techniques; Three-Dimensional Image; Three-Dimensional Imaging; Time; Training; Work; assay development; college; diffraction of light; fluorescence imaging; fluorescence microscope; instrumentation; light microscopy; optical imaging; three dimensional structure

 DESCRIPTION (provided by applicant): The present application is to request a high speed multicolor 3D structured illumination super-resolution fluorescence microscope (SIM) for the Integrated Microscopy Core (IMC), a long standing shared instrumentation facility at the Baylor College of Medicine. The requested OMX" system with the Blaze" SIM- module from Applied Precision is the first commercially available super-resolution system capable of acquiring 3D images with lateral and axial resolutions at least two times smaller than the classical limit imposed by the diffraction of light, at speeds compatible with recording fast dynamic events in live cells and at multiple wavelengths by the use of multiple high resolution-high sensitivity CMOS cameras. The system allows 3D imaging at a level of detail not possible with confocal microscopes or wide field 3D deconvolution systems. In contrast to other high resolution imaging tools commonly used in biology, such as electron and atomic force microscopes, specialized near-field probe scanning microscopes and other newly developed and commercially available far-field super resolution techniques such as STED, PALM and STORM, the OMX 3D SIM system is able to achieve multicolor fluorescence imaging and is also built for image acquisition speeds and low levels of light exposure compatible with live cell studies. Collectively, the OMX overcomes spatial and temporal limitations presented by other microscopy systems. In addition, the OMX: a) works with conventional fluorochromes, b) does not present the geometrical limitations to the sample present in other high resolution systems, c) does not require special mounting and preparation techniques, and d) is not more complicated to operate than other commercially available microscopy systems already in use in the IMC. The OMX is the fastest 3D SIM system commercially available and is the only one that takes advantage of multiple liquid cooled sCMOS cameras (up to 4) for multi-wavelength imaging. The OMX will be housed in completely new renovated spaces for the IMC and adjacent PI's laboratory. An OMX addition to the IMC, an open access optical imaging facility at Baylor supporting light and electron microscopy, will synergize with and extend current state of the art imaging and image analysis resources, including hardware/software training and assay development support.

 描述（由申请人提供）：本申请要求为集成显微镜核心（IMC）提供高速多色 3D 结构照明超分辨率荧光显微镜（SIM），IMC 是贝勒医学院长期存在的共享仪器设施。所要求的带有 Applied Precision 的 Blaze" SIM 模块的 OMX" 系统是第一个商用超分辨率系统，能够采集横向和轴向分辨率至少比光衍射所施加的经典极限小两倍的 3D 图像，速度与记录活细胞中的快速动态事件兼容，并且通过使用多个高分辨率高灵敏度 CMOS 相机以多个波长进行记录。该系统可实现共焦显微镜或宽视场 3D 反卷积系统无法实现的详细程度的 3D 成像。与生物学中常用的其他高分辨率成像工具（例如电子和原子力显微镜、专用近场探针扫描显微镜以及其他新开发和商用的远场超分辨率技术（例如 STED、PALM 和 STORM））相比，OMX 3D SIM 系统能够实现多色荧光成像，并且还专为与活细胞兼容的图像采集速度和低曝光水平而设计 研究。总的来说，OMX 克服了其他显微镜系统带来的空间和时间限制。此外，OMX：a) 适用于传统荧光染料，b) 不存在其他高分辨率系统中样品的几何限制，c) 不需要特殊的安装和制备技术，d) 操作起来并不比 IMC 中已使用的其他市售显微镜系统更复杂。 OMX 是市面上速度最快的 3D SIM 系统，也是唯一一款利用多个液冷 sCMOS 相机（最多 4 个）进行多波长成像的系统。 OMX 将安置在 IMC 和相邻 PI 实验室全新装修的空间中。 OMX 是贝勒支持光学和电子显微镜的开放式光学成像设施 IMC 的补充，将与当前最先进的成像和图像分析资源相协同并扩展，包括硬件/软件培训和分析开发支持。