VivaCT 40 Scanner

VivaCT 40 扫描仪

• 批准号: 8052441
• 负责人: LAURIE Hollis GLIMCHER
• 金额: $ 39.67万
• 依托单位: HARVARD SCHOOL OF PUBLIC HEALTH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-15 至 2012-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052441
• 关键词: Anesthesia procedures; Animal Barrier Facility; Animals; Arthritis; Behavior; Biocompatible Materials; Biomedical Research; Bone remodeling; Boston; Cartilage Matrix; Chondrocytes; Communities; Computers; Contract Services; Data Analyses; Dental Schools; Development; Evaluation; Faculty; Funding; Genetic; Genetically Engineered Mouse; Human; Individual; Isoflurane; Joints; Learning; Malignant Neoplasms; Medical; Medicine; Minor; Modeling; Molecular; Mus; Osteoblasts; Osteoclasts; Osteoporosis; Pathway interactions; Pre-Clinical Model; Process; Public Health; Public Health Schools; Rodent; Site; Skeletal system; Stress; System; Techniques; Time; Validation; X-Ray Computed Tomography; base; bone; bone loss; data acquisition; improved; in vivo; instrument; interest; member; mouse model; preclinical study; public health relevance; skeletal; skeletal disorder

DESCRIPTION (provided by applicant): We are requesting funding for an In Vivo micro-computerized tomography (<CT) system from SCANCO Medical (Viva-CT 40) to be sited in the barrier animal facility at the Harvard School of Public Health (HSPH) for use by the biomedical research community at HSPH and the Harvard School of Dental Medicine (HSDM) in Boston. This system consists of the Viva-CT 40 scanner, two computers for data acquisition and analysis, and an isoflurane based anesthesia system. The In Vivo <CT will be primarily used to evaluate genetically engineered mice in established and developmental models of bone loss and accrual as well as arthritis. Bone and joints are remarkable biomaterials that model and remodel to grow, preserve structural integrity and adapt to stress. This dynamic process is controlled by bone forming osteoblasts, bone resorbing osteoclasts and cartilage matrix secreting chondrocytes. An imbalance between catabolic and anabolic activity within bone and joints contributes to some of the most common public health problems including osteoporosis, arthritis and cancer. Much has been learned about the molecular control of bone remodeling by the analysis of genetically engineered mouse models of these human conditions. Importantly, humans and rodents share the majority of pathways that control osteoblast, osteoclast and chondrocyte behavior. The relatively recent availability of In Vivo <CT machines has made it possible for biologists interested in the skeletal system to follow skeletal parameters in the same mouse over time. This technique could vastly improve the quality of the currently funded studies from the major (Glimcher, Olsen and Baron) and minor (Hotamisligil, Aliprantis, Jones, Lankse, and Li) users included in this proposal who each use mouse genetics to probe the skeletal system. The Viva CT 40 will be incorporated into the existing <CT facility at HSDM where we now routinely perform Ex Vivo <CT. A strong commitment from the administration at HSPH completes this application by assuring a long- term service contract for the instrument as well as support for technical assistance. In summary, the Viva CT 40 will allow us to longitudinally compare bone parameters in the same mouse over time thereby substantially reducing the number of animals needed for robust validation of preclinical studies and facilitating the timely completion of our PHS funded projects. Public Health Relevance: Ten faculty members at HSPH and HSDM who each use mouse genetics to probe the skeletal system are requesting funding for an In Vivo CAT scan system from SCANCO Medical (Viva-CT 40). This instrument allows the evaluation of individual genetically engineered mice in models of bone loss and accrual over time and will provide robust validation of preclinical models of human skeletal diseases such as osteoporosis and arthritis.

描述（由申请人提供）：我们正在申请资助SCANCO Medical的体内微型计算机断层扫描（<CT）系统（Viva-CT 40），该系统将被放置在哈佛公共卫生学院（HSPH）的屏障动物设施中，供HSPH的生物医学研究界和波士顿哈佛牙科医学院（HSDM）使用。该系统由Viva-CT 40扫描仪、两台用于数据采集和分析的计算机以及基于异氟烷的麻醉系统组成。In Vivo <CT将主要用于评估基因工程小鼠在已建立和发育的骨质流失、骨质增生以及关节炎模型中的表现。骨和关节是一种重要的生物材料，可以通过建模和重塑来生长、保持结构完整性和适应压力。这一动态过程是由成骨细胞、骨吸收破骨细胞和软骨基质分泌软骨细胞控制的。骨骼和关节内分解代谢和合成代谢活动之间的不平衡导致了一些最常见的公共健康问题，包括骨质疏松症、关节炎和癌症。通过对这些人类条件下的基因工程小鼠模型的分析，已经了解了许多关于骨重塑的分子控制。重要的是，人类和啮齿动物共享大部分控制成骨细胞、破骨细胞和软骨细胞行为的途径。相对较新的体内CT机器的可用性使得对骨骼系统感兴趣的生物学家有可能随着时间的推移跟踪同一只小鼠的骨骼参数。这项技术可以极大地提高目前资助的主要（Glimcher， Olsen和Baron）和次要（Hotamisligil, Aliprantis, Jones， Lankse和Li）研究的质量，这些研究包括在本提案中，他们每个人都使用小鼠遗传学来探测骨骼系统。Viva CT 40将被整合到HSDM现有的<CT设备中，我们现在经常在那里进行离体<CT。HSPH管理部门的坚定承诺，通过确保仪器的长期服务合同以及技术援助支持，完成了这项申请。总之，Viva CT 40将使我们能够纵向比较同一只小鼠的骨骼参数，从而大大减少了临床前研究验证所需的动物数量，并促进了我们PHS资助项目的及时完成。