VivaCT 40 Scanner

VivaCT 40 扫描仪

• 批准号: 8052441
• 负责人: LAURIE Hollis GLIMCHER
• 金额: $ 39.67万
• 依托单位: HARVARD SCHOOL OF PUBLIC HEALTH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-15 至 2012-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052441
• 关键词: Anesthesia procedures; Animal Barrier Facility; Animals; Arthritis; Behavior; Biocompatible Materials; Biomedical Research; Bone remodeling; Boston; Cartilage Matrix; Chondrocytes; Communities; Computers; Contract Services; Data Analyses; Dental Schools; Development; Evaluation; Faculty; Funding; Genetic; Genetically Engineered Mouse; Human; Individual; Isoflurane; Joints; Learning; Malignant Neoplasms; Medical; Medicine; Minor; Modeling; Molecular; Mus; Osteoblasts; Osteoclasts; Osteoporosis; Pathway interactions; Pre-Clinical Model; Process; Public Health; Public Health Schools; Rodent; Site; Skeletal system; Stress; System; Techniques; Time; Validation; X-Ray Computed Tomography; base; bone; bone loss; data acquisition; improved; in vivo; instrument; interest; member; mouse model; preclinical study; public health relevance; skeletal; skeletal disorder

DESCRIPTION (provided by applicant): We are requesting funding for an In Vivo micro-computerized tomography (<CT) system from SCANCO Medical (Viva-CT 40) to be sited in the barrier animal facility at the Harvard School of Public Health (HSPH) for use by the biomedical research community at HSPH and the Harvard School of Dental Medicine (HSDM) in Boston. This system consists of the Viva-CT 40 scanner, two computers for data acquisition and analysis, and an isoflurane based anesthesia system. The In Vivo <CT will be primarily used to evaluate genetically engineered mice in established and developmental models of bone loss and accrual as well as arthritis. Bone and joints are remarkable biomaterials that model and remodel to grow, preserve structural integrity and adapt to stress. This dynamic process is controlled by bone forming osteoblasts, bone resorbing osteoclasts and cartilage matrix secreting chondrocytes. An imbalance between catabolic and anabolic activity within bone and joints contributes to some of the most common public health problems including osteoporosis, arthritis and cancer. Much has been learned about the molecular control of bone remodeling by the analysis of genetically engineered mouse models of these human conditions. Importantly, humans and rodents share the majority of pathways that control osteoblast, osteoclast and chondrocyte behavior. The relatively recent availability of In Vivo <CT machines has made it possible for biologists interested in the skeletal system to follow skeletal parameters in the same mouse over time. This technique could vastly improve the quality of the currently funded studies from the major (Glimcher, Olsen and Baron) and minor (Hotamisligil, Aliprantis, Jones, Lankse, and Li) users included in this proposal who each use mouse genetics to probe the skeletal system. The Viva CT 40 will be incorporated into the existing <CT facility at HSDM where we now routinely perform Ex Vivo <CT. A strong commitment from the administration at HSPH completes this application by assuring a long- term service contract for the instrument as well as support for technical assistance. In summary, the Viva CT 40 will allow us to longitudinally compare bone parameters in the same mouse over time thereby substantially reducing the number of animals needed for robust validation of preclinical studies and facilitating the timely completion of our PHS funded projects. Public Health Relevance: Ten faculty members at HSPH and HSDM who each use mouse genetics to probe the skeletal system are requesting funding for an In Vivo CAT scan system from SCANCO Medical (Viva-CT 40). This instrument allows the evaluation of individual genetically engineered mice in models of bone loss and accrual over time and will provide robust validation of preclinical models of human skeletal diseases such as osteoporosis and arthritis.

描述(由申请者提供)：我们正在为SCANCO Medical(VIVA-CT 40)的在体微型计算机断层扫描(&lt；CT)系统申请资金，该系统将安装在哈佛公共卫生学院(HSPH)的屏障动物设施中，供HSPH的生物医学研究社区和波士顿的哈佛大学牙科医学院(HSDM)使用。该系统由Viva-CT40扫描仪、两台用于数据采集和分析的计算机以及一套基于异氟醚的麻醉系统组成。体内CT将主要用于评估已建立的和发育的骨丢失、骨积累和关节炎模型中的基因工程小鼠。骨和关节是值得注意的生物材料，可以模拟和重塑以生长、保持结构完整性和适应压力。这一动态过程受成骨细胞、骨吸收破骨细胞和软骨基质分泌软骨细胞的控制。骨骼和关节内分解代谢和合成代谢活动之间的失衡导致了一些最常见的公共健康问题，包括骨质疏松症、关节炎和癌症。通过对这些人类条件下的基因工程小鼠模型的分析，已经了解了许多关于骨骼重塑的分子控制。重要的是，人类和啮齿动物共享大多数控制成骨细胞、破骨细胞和软骨细胞行为的途径。相对较新的In Vivo&lt；CT机器的出现，使得对骨骼系统感兴趣的生物学家有可能随着时间的推移跟踪同一只小鼠的骨骼参数。这项技术可以极大地提高目前由主要用户(Glimcher、Olsen和Baron)和次要用户(Hotamisligil、Aliprantis、Jones、Lankse和Li)资助的研究的质量，这些用户都使用小鼠遗传学来探测骨骼系统。Viva CT 40将被合并到HSDM现有的CT设施中，我们现在经常在那里进行Ex Vivo&lt；CT。HSPH管理部门的坚定承诺确保了仪器的长期服务合同以及对技术援助的支持，从而完成了这一申请。总而言之，Viva CT 40将允许我们随着时间的推移对同一小鼠的骨骼参数进行纵向比较，从而大幅减少稳健验证临床前研究所需的动物数量，并促进我们的小灵通资助项目的及时完成。 公共卫生相关性：HSPH和HSDM的10名教职员工分别使用老鼠遗传学来探测骨骼系统，他们正在向SCANCO Medical(Viva-CT 40)申请资助活体CAT扫描系统。该仪器允许在骨丢失和随时间增长的模型中对单个基因工程小鼠进行评估，并将对人类骨骼疾病(如骨质疏松症和关节炎)的临床前模型提供强有力的验证。