Typhoon 9400 Variable Mode Imager for Quantitative Detection of Biomolecules

描述（由申请人提供）：宾夕法尼亚大学（包括宾夕法尼亚大学医学院、宾夕法尼亚大学兽医学院和费城儿童医院）的研究人员越来越多地参与研究项目，这些项目需要复杂、敏感和高效的检测技术来研究基因表达、蛋白质生物合成、代谢途径和分子信号事件。这项技术对这些机构中许多正在进行的NIH资助的研究项目的生产力变得尤为重要。此外，各种迹象表明，在不久的将来，对高度复杂的成像技术的需求将会增加。目前，该小组的研究人员一直在使用各种传统的检测技术，包括CCD相机化学发光，标准磷光仪技术，x射线胶片和基于线性扫描仪的图像采集和量化设备。另外，这些技术中的每一种都缺乏高通量、足够的灵敏度或足够的空间分辨率，这是当今复杂的细胞信号和蛋白质组学方法所必需的。在本申请中，我们建议常规使用能够检测各种报告技术的台式分析仪器，这将极大地促进NIH资助的一系列研究项目的执行，这些研究项目目前正在由参与该联盟的七个主要用户进行。为了解决这个问题，我们申请购买最先进的、多模态、高通量成像系统，该系统将成熟的存储磷光体放射自显影技术与四色荧光标记和增强的化学发光检测相结合，用于DNA、RNA和蛋白质样品中的生物分子的定量分析。拟议的设备，台风9400可变模式成像仪，将被整合到已经在兽医学院医学实验室建立的蛋白质和分子生物学核心中，以促进来自这些学院一系列研究学科的一组合作研究人员不断增长的生物分子成像需求。

DESCRIPTION (provided by applicant): Researchers at the University of Pennsylvania including the University Of Pennsylvania School Of Medicine, the University Of Pennsylvania School Of Veterinary Medicine and the Children's Hospital of Philadelphia are increasing engaged in research projects that require sophisticated, sensitive, and highly efficient detection techniques for the study gene expression, protein biosynthesis, metabolic pathways, and molecular signaling events. This technology is becoming particularly critical to the productivity of many ongoing NIH funded research projects at these institutions. Furthermore, there is every indication that demand for highly sophisticated imaging techniques will increase in the near future. Currently, investigators in this group have been using a variety of traditional detection techniques which include CCD camera chemiluminescence, standard phosphorimager technology, x-ray film, and linear scanner based image acquisition and quantization devices. Separately, each of these techniques suffers from either a lack of high-throughput, sufficient sensitivity, or adequate spatial resolution required for today's sophisticated cell signaling and proteomics approaches. In this application, we propose that routine access to a bench top analytical instrument capable of detecting a wide range of reporter technologies would greatly facilitate the execution of a spectrum of NIH funded research projects currently being pursued by the seven major users participating in this consortium. To address this, we therefore make application to purchase a state of the art, multimodal, high-throughput imaging system which unites proven storage phosphor autoradiography technology with four color fluorescence labeling and enhanced chemiluminescence detection for the quantitative analysis of biomolecules in DNA, RNA, and protein samples. The proposed equipment, a Typhoon 9400 Variable Mode Imager, will be integrated into the Protein and Molecular Biology Core already established within the Department of Medicine Laboratories housed in the School of Veterinary Medicine for the purpose of facilitating the growing biomolecular imaging needs of a group of collaborative investigators drawn from a range of research disciplines at these Schools.