Phoenix / X-ray nanoTOM

菲尼克斯 / X 射线 nanoTOM

• 批准号: 8051356
• 负责人: KARL J JEPSEN
• 金额: $ 56.15万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-01-13
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051356
• 关键词: 3-Dimensional; Aging; Attenuated; Biological; Biomechanics; Cardiovascular Diseases; Cardiovascular system; Collaborations; Communities; Craniosynostosis; Data; Development; Disease; Drug or chemical Tissue Distribution; Femur; Funding; Genetic; Growth; Healthcare; Histology; Human; Image; Institution; Marketing; Methods; Modeling; Mus; Musculoskeletal; Musculoskeletal Diseases; Oryctolagus cuniculus; Outcome Study; Pathogenesis; Request for Proposals; Research; Research Personnel; Resolution; Resource Sharing; Roentgen Rays; Sampling; Skeleton; Spatial Distribution; Specimen; Structure; System; Technology; Time; Tissues; Whales; X-Ray Computed Tomography; base; bone; bone imaging; functional outcomes; macrophage; medical schools; mineralization; mouse model; nanoparticle; postnatal; prenatal; programs; public health relevance; research facility; skeletal; submicron; tomography; trait

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a nanoComputed Tomography system (nanotom(R); phoenix|x- ray, marketed by GE Healthcare) with the intent of significantly advancing the research programs of over a dozen federally funded investigators at the Mount Sinai School of Medicine. This technology will facilitate our ability to accurately and efficiently quantify aspects of the 3D arrangement or distribution of tissues that are important for assessing functional outcomes and for studying the pathogenesis of disease. For musculoskeletal research, our investigators show that understanding the development of traits during pre- and postnatal growth is central to advancing our understanding of the genetic basis of skeletal fragility and craniosynostosis. However, the growing skeleton remains an extremely challenging structure to image using conventional microCT systems because of the small structural features and low mineralization. Further, researchers conducting large-scale research on the 3D arrangement of trabeculae in human bone and whale bone are limited to low resolution CT images or 2D histology, neither of which will advance their programs. For cardiovascular research, efforts to use nanoparticles to identify the spatial distribution of macrophages within cardiovascular tissues are currently limited to a rabbit model because we lack an available high resolution imaging system to move this research to the mouse model. Consequently, our investigators must rely on destructive, time-consuming, and expensive histological methods to acquire data. Although histology provides valuable cellular information, the structural readouts are limited to 2D information, and thus impair our ability to acquire the 3D spatial information needed to advance our scientific programs. Our investigators need an extremely versatile, multi-scale imaging system to generate high resolution 3-dimensional (3D) images of skeletal and cardiovascular structures ranging in size from 1- 100 mm. We propose to purchase the phoenix|x-ray nanotom(R) because this system is a unique ex vivo computed tomography system that non-destructively acquires extremely high resolution (0.5 mm) 3D images of x-ray attenuating samples. The nanotom can operate in four modes, including a nanofocus mode (0.5mm voxel size) for small biological samples (e.g., postnatal mouse bone) and a high power mode (180kV, 15 Watts) for large biological specimens (e.g., proximal human femora). This single system will allow us to do multi-scale imaging and to offset the heavy demand placed on our existing and aging microCT system. These two systems will be incorporated into a Shared Research Facility to better support the imaging needs of MSSM researchers. Relevance: The non-destructive imaging and accurate quantification provided by the phoenix|x-ray nanotom(R) allows specimens to be used for subsequent analyses such as histomorphometry and biomechanics, providing a more complete characterization than otherwise possible. Public Health Relevance: This proposal is a request to purchase a phoenix|x-ray nanotom(r), a uniquely versatile imaging system that will enhance our scientific research program by obtaining 3D images of biological structures at sub-micron resolution from which our users can quantify traits leading to a better understanding of functional outcomes and the pathogenesis of musculoskeletal and cardiovascular diseases. This multi-scale imaging system will be incorporated into a Shared Resource Facility to make it available to the Mount Sinai research community. The nanotom will provide state-of-the-art high resolution multi-scale imaging capabilities that will enhance collaborations among investigators at our institution and identify connections to diseases and treatments that would otherwise have been overlooked.

描述（由申请人提供）：该提案请求资金购买纳米计算机断层扫描系统（nanotom(R)；phoenix|x-ray，由 GE Healthcare 销售），旨在显着推进西奈山医学院十几名联邦资助研究人员的研究项目。这项技术将有助于我们准确有效地量化组织 3D 排列或分布的各个方面，这对于评估功能结果和研究疾病的发病机制非常重要。对于肌肉骨骼研究，我们的研究人员表明，了解出生前和出生后生长过程中特征的发展对于增进我们对骨骼脆弱和颅缝早闭的遗传基础的理解至关重要。然而，由于结构特征小且矿化度低，不断生长的骨骼对于使用传统 microCT 系统成像仍然是一个极具挑战性的结构。此外，研究人员对人骨和鲸骨中小梁的 3D 排列进行大规模研究仅限于低分辨率 CT 图像或 2D 组织学，这两者都无法推进他们的计划。对于心血管研究，使用纳米粒子来识别心血管组织内巨噬细胞的空间分布的努力目前仅限于兔子模型，因为我们缺乏可用的高分辨率成像系统来将该研究转移到小鼠模型。因此，我们的研究人员必须依靠破坏性、耗时且昂贵的组织学方法来获取数据。尽管组织学提供了有价值的细胞信息，但结构读数仅限于 2D 信息，从而削弱了我们获取推进科学计划所需的 3D 空间信息的能力。我们的研究人员需要一个极其通用的多尺度成像系统来生成尺寸范围为 1-100 毫米的骨骼和心血管结构的高分辨率 3 维 (3D) 图像。我们建议购买 phoenix|x 射线 nanotom(R)，因为该系统是独特的离体计算机断层扫描系统，可以非破坏性地获取 X 射线衰减样品的极高分辨率 (0.5 mm) 3D 图像。 nanotom 可以在四种模式下运行，包括用于小型生物样本（例如，产后小鼠骨骼）的纳米焦点模式（0.5mm 体素大小）和用于大型生物样本（例如，近端人类股骨）的高功率模式（180kV，15 瓦）。这个单一系统将使我们能够进行多尺度成像，并抵消对我们现有且老化的 microCT 系统的巨大需求。这两个系统将被纳入一个共享研究设施，以更好地支持 MSSM 研究人员的成像需求。相关性： phoenix|x 射线 nanotom(R) 提供的无损成像和精确定量允许将样本用于后续分析，例如组织形态测量和生物力学，从而提供比其他方式更完整的表征。 公共健康相关性：本提案请求购买 phoenix|x 射线 nanotom(r)，这是一种独特的多功能成像系统，它将通过获取亚微米分辨率的生物结构 3D 图像来增强我们的科学研究计划，我们的用户可以从中量化特征，从而更好地了解功能结果以及肌肉骨骼和心血管疾病的发病机制。这种多尺度成像系统将被纳入共享资源设施，以供西奈山研究界使用。纳米断层扫描将提供最先进的高分辨率多尺度成像能力，这将加强我们机构研究人员之间的合作，并确定与疾病和治疗的联系，否则这些可能会被忽视。