Structural Biology Shared Service

结构生物学共享服务

• 批准号: 9145400
• 负责人: David Joseph Weber
• 金额: $ 12.55万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-04 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9145400
• 关键词: Address; Americas; Award; Baltimore; Biological; Biometry; Cancer Center; Cancer Center Support Grant; Cigarette; Computer Assisted; Consultations; County; Crystallization; Data; Data Collection; Databases; Deposition; Development; Doctor of Philosophy; Drug Design; Enzymes; Flow Cytometry; Funding; Genomics; Grant; Head; Hormone Responsive; Human; Image; Institutes; Institution; Investigational Therapies; Journals; Laboratories; Malignant Neoplasms; Maryland; Medical; Medical center; Methodology; Methods; Mission; Molecular; Molecular Biology; Molecular Mechanisms of Action; Molecular Probes; Nature; Nuclear Magnetic Resonance; Pathology; Peer Review; Pharmaceutical Chemistry; Pharmacy Schools; Population Sciences; Preparation; Protein Databases; Proteins; Public Health Schools; Publications; Publishing; Reader; Research; Research Personnel; Resolution; Resources; Robotics; Roentgen Rays; Sampling; School Dentistry; Services; Structure; System; Therapeutic; Training; Translational Research; United States; United States National Academy of Sciences; United States National Institutes of Health; Universities; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; abstracting; acronyms; biobank; collaborative environment; college; computerized data processing; design; drug candidate; high throughput screening; instrument; instrumentation; investigator training; medical schools; member; neoplasm immunotherapy; novel; pre-clinical; programs; small molecule inhibitor; small molecule libraries; structural biology; training opportunity; tumor immunology; virology

6.0 Abstract: Structural Biology Shared Service The Structural Biology Shared Service (SBSS) provides investigators with the facilities and expertise to collect and interpret molecular structural information (nuclear magnetic resonance [NMR], X-ray crystallography) to understand the molecular mechanism(s) of action of proteins and enzymes that contribute to cancer. The SBSS also facilitates identification of molecular agents (e.g., candidate drugs, molecular probes) that can be used to study the function of cancer targets and may have therapeutic potential. To achieve this mission, the SBSS implements a structural biology approach. This includes isolation and characterization of samples for NMR and X ray and application of state-of-the-art data collection methods in a collaborative environment that facilitates access by UMGCC investigators. In 2014, 36 UMGCC investigators used the SBSS. These investigators deposited 84 high-resolution structures into the protein database in addition to publishing in high- impact journals. The efforts of the SBSS have facilitated development of several novel, cancer target–specific small-molecule inhibitors. Interactions between the SBSS and investigators from multiple UMGCC programs have facilitated the integration of structural biology into the translational research efforts of UMGCC. Also of significance, the SBSS installed and commissioned a new 950 MHz NMR spectrometer purchased through an $8 million NIH Shared Instrumentation Grant. This is the only such instrument available in an academic institution in the United States and represents the largest Shared Instrumentation Grant awarded by NIH. The SBSS has also expanded and upgraded X-ray diffraction and robotic crystallization resources to enhance the use of these resources. In 2014, the SBSS supported 36 Cancer Center members spanning all 5 research programs (14 percent of all UMGCC members); 53 percent of UMGCC users have peer-reviewed funding. The SBSS supports many cancer-related publications annually, many in high-impact journals including Proceedings of the National Academy of Sciences of the United States of America, Journal of Medicinal Chemistry, and Nature Structural & Molecular Biology.

6.0摘要：结构生物学共享服务 结构生物学共享服务（SBSS）为研究人员提供设施和专业知识， 并解释分子结构信息（核磁共振[NMR]，X射线晶体学）， 了解导致癌症的蛋白质和酶的分子作用机制。的 SBSS还有助于识别分子试剂（例如，候选药物，分子探针）， 用于研究癌症靶点的功能，并可能具有治疗潜力。为了完成这一使命， SBSS采用结构生物学方法。这包括样品的分离和表征， NMR和X射线以及在协作环境中应用最先进的数据收集方法， 方便UMGCC调查人员访问。2014年，36名UMCCC调查员使用了SBSS。这些 研究人员将84个高分辨率结构存入蛋白质数据库， 影响期刊。SBSS的努力促进了几种新型癌症靶向特异性药物的开发。 小分子抑制剂。SBSS与来自多个UMCCC项目的研究者之间的互动 促进了结构生物学与UMCCC转化研究工作的整合。评价很 具有重要意义的是，SBSS安装并调试了一台新的950 MHz NMR光谱仪，该光谱仪是通过 800万美元NIH共享仪器补助金。这是唯一一个这样的仪器可在学术 这是美国最大的共享仪器基金，也是NIH授予的最大的共享仪器基金。的 SBSS还扩大和升级了X射线衍射和机器人结晶资源，以提高 利用这些资源。 2014年，SBSS支持了36名癌症中心成员，涵盖所有5个研究项目（占所有项目的14 UMCCC成员）; 53%的UMCCC用户拥有同行评审的资金。SBSS支持许多 癌症相关的出版物，许多在高影响力的期刊，包括美国国家科学院院刊 美国科学院、药物化学杂志和自然结构与 分子生物学