ROLE OF NATURAL KILLER T (NKT) LYMPHOCYTES IN SOOTY MANGABEYS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 自然杀伤T（NKT）淋巴细胞是一个独特的T淋巴细胞亚群，具有不变的TCR Valpha链，并识别由非多态性CD 1d分子呈递的配体。它们是先天免疫系统的早期效应物，并且可以分泌大量Th-1和Th-2型细胞因子，包括IL-10，其可能在调节HIV和SIV感染中的免疫活化中起作用。煤烟型白眉猴是SIV的天然宿主，是非进行性的;这种SIV感染的关键区别特征是没有异常的免疫激活。在这项研究中，我们希望确定自然感染SIV的白眉猴中的NKT细胞是否负责这种异常免疫激活的缺乏。对50只SIV阴性和50只SIV阳性白眉猴的外周血进行流式细胞术评估，发现在大约50%的白眉猴中循环Valpha 24阳性CD 1d NKT淋巴细胞的可检测频率。SIV感染的自然和非自然宿主中NKT淋巴细胞的比较正在进行中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Natural Killer T(NKT) lymphocytes are a unique subset of T lymphocytes that have an invariant TCR Valpha chain and recognize ligands presented by the nonpolymorphic CD1d molecule. They are early effectors of the innate immune system, and can secrete large amounts of both Th-1 and Th-2 type cytokines, including IL-10 that may play a role in modulating immune activation in HIV and SIV infection. Sooty mangabeys are a natural host of SIV that is non-progressive; key differentiating feature of this SIV infection is the absence of aberrant immune activation. .In this investigation we hope to determine if NKT cells in naturally SIV-infected sooty mangabeys are responsible for this absence of aberrant immune activation. .Flow cytometric evaluation of peripheral blood in 50 SIV negative and 50 SIV positive sooty mangabeys has revealed detectable frequencies of circulating Valpha 24positive CDld NKT lymphocytes in approximately 50% of sooty mangabeys. Comparison of NKT lymphocytes in natural and non-natural hosts of SIV infection is in progress.