CHARACTERIZATION OF MHC CLASS I ALLELES IN SOOTY MANGABEYS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 MHC I类限制性细胞毒性T淋巴细胞（CTL）在人类免疫缺陷病毒（HIV）和猿猴免疫缺陷病毒（SIV）感染的控制中起重要作用。白眉猴（Cercocebus atys; Ceat）是SIV的天然宿主，是研究AIDS发病机制的重要模型动物。我们以前已经确定了几个免疫显性SIV特异性CTL表位在strokabeys和显示，CD 8阳性T淋巴细胞抑制SIV复制在体内自然SIV感染strokabeys。为了鉴定免疫优势CTL表位的MHC I类限制性，我们克隆并鉴定了白眉猴的MHC I类基因。 这些研究将有助于评估CTL在维持非致病性SIV感染中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. MHC class I-restricted cytotoxic T-lymphocytes (CTL) play an important role in controlling human immunodeficiency virus and simian immunodeficiency virus (SIV) infection. As a natural host of SIV, sooty mangabeys (Cercocebus atys; Ceat) are a valuable model for the study of AIDS pathogenesis. We have previously defined several immunodominant SIV-specific CTL epitopes in sooty mangabeys and shown that CD8-positive T lymphocytes inhibit SIV replication in vivo in naturally SIV-infected sooty mangabeys. In order to identify the MHC class I restriction of immunodominant CTL epitopes, we have cloned and characterized MHC class I genes of sooty mangabeys. These studies will facilitate evaluation of the role of CTL in maintaining nonpathogenic SIV infection in sooty mangabeys.