CHARACTERIZATION OF MHC CLASS I ALLELES IN SOOTY MANGABEYS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 MHC-I类限制性细胞毒性T淋巴细胞(CTL)在控制人类免疫缺陷病毒(HIV)和猴免疫缺陷病毒(SIV)感染中发挥着重要作用。作为SIV的自然宿主，烟夜蛾(Cercocebus Atys；Ceat)是研究艾滋病发病机制的有价值的模型。我们以前已经在煤烟芒果中定义了几个免疫优势的SIV特异性CTL表位，并表明CD8+T淋巴细胞在自然感染SIV的煤烟芒果体内抑制SIV复制。为了鉴定免疫优势CTL表位的MHC-I类限制性，我们克隆并鉴定了煤质山竹MHC-I类基因。这些研究将有助于评估CTL在维持煤烟芒果非致病性SIV感染中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. MHC class I-restricted cytotoxic T-lymphocytes (CTL) play an important role in controlling human immunodeficiency virus and simian immunodeficiency virus (SIV) infection. As a natural host of SIV, sooty mangabeys (Cercocebus atys; Ceat) are a valuable model for the study of AIDS pathogenesis. We have previously defined several immunodominant SIV-specific CTL epitopes in sooty mangabeys and shown that CD8-positive T lymphocytes inhibit SIV replication in vivo in naturally SIV-infected sooty mangabeys. In order to identify the MHC class I restriction of immunodominant CTL epitopes, we have cloned and characterized MHC class I genes of sooty mangabeys. These studies will facilitate evaluation of the role of CTL in maintaining nonpathogenic SIV infection in sooty mangabeys.