Biofilm Growth and Analysis Core

生物膜生长和分析核心

• 批准号: 8115948
• 负责人: Jeffrey Lee Bose
• 金额: $ 12.99万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8115948
• 关键词: Animals; Bacteria; Biology; Communities; Complex; DNA; Detection; Development; Ensure; Equipment; Gene Expression; Genomics; Genus staphylococcus; Goals; Growth; Growth and Development function; Housing; Inflammation Mediators; Instruction; Liquid substance; Maintenance; Measures; Microbial Biofilms; Nutrient; Organism; Oxygen; Participant; Physiological; Polysaccharides; Production; Proteins; Protocols documentation; Staphylococcal Infections; Structure; Surface; Suspension substance; Suspensions; System; Techniques; Technology; Time; Waste Products; instrument; instrumentation; research study

It has only recently been recognized that bacteria, including the staphylococci, often exist as sessile organisms, attached to surfaces as highly complex and organized communities termed biofilms. These communifies are encased in matrices comprised of polysaccharides, proteins, and/or genomic DNA and are exposed to a remarkable variety of environmental conditions. Given that the study of bacterial biofilm is still relatively new, especially when considering staphylococcal biofllm, there exists a wide range of techniques and strategies to grow and analyze these complex structures. Not surprisingly, this variability makes it difficult to compare results from experiment to experiment and inhibits progress in understanding biofllm biology. To provide uniform and consistent strategies for the growth and analysis of biofilm, we propose a Core that will provide common biofllm growth equipment and analytical strategies for the participants of this PPG. Specific Aim 1 of this Core will be to house and maintain equipment needed for biofilm growth and will include the purchase and maintenance of biofllm growth systems that will be extensively used by all four projects involved in this PPG. In addition, it will also maintain an anaerobic chamber for use in Projects 1 and 2. Specific Aim will develop and maintain standardized protocols for biofilm growth and will serve to provide the protocols and expertise needed to produce consistent and reproducible biofllm results for all four Projects. Finally, Specific Aim 3 will be to acquire the technology needed for the analysis and impact of biofilm on the host. The goals of this aim will be to provide a microprobe detection system needed to detect fluctuations in speciflc metabolites produced during biofilm development, as well as a quanfitafive real-fime PCR system to measure changes in gene expression. It will also provide a Luminex Multi-analyte Detecfion system to follow the production of multiple inflammatory mediators during staphylococcal infection. Combined, these aims will make available the fundamental technology and expertise needed to carry-out all four projects described in this PPG and will help to ensure that reproducible results are obtained from project to project. RELEVANCE (See instructions):

直到最近才认识到，包括葡萄球菌在内的细菌通常存在为无柄 有机体，与高度复杂和有组织的社区相连，称为生物膜。这些 沟通包含在由多糖，蛋白质和/或基因组DNA组成的基质中，并且是 暴露于各种各样的环境条件。鉴于细菌生物膜的研究仍然是 相对较新，尤其是在考虑葡萄球菌生物流体时，存在多种技术 以及成长和分析这些复杂结构的策略。毫不奇怪，这种可变性使得 很难比较实验的结果，并抑制了理解BioFllm的进展 生物学。为了为生物膜的生长和分析提供统一和一致的策略，我们提出 将为参与者提供常见的Biofllm增长设备和分析策略的核心 ppg。该核心的特定目的1将是容纳和维护生物膜增长所需的设备 并将包括购买和维护Biofllm增长系统，所有这些系统都将被所有人广泛使用 该PPG涉及的四个项目。此外，它还将维护一个厌氧室，用于项目1 2。具体目标将开发和维护生物膜增长的标准化协议，并将服务 为所有人提供一致且可重复的生物福利结果所需的协议和专业知识 四个项目。最后，具体目标3将是获取分析所需的技术， 生物膜对宿主的影响。该目标的目标是提供微探测系统 需要检测生物膜发育过程中产生的特异性代谢产物的波动，以及 Quanfitafive实录PCR系统测量基因表达的变化。它还将提供一个Luminex 多分析物检测系统遵循在多个炎症介体的产生期间的产生 葡萄球菌感染。这些目标结合在一起，将使基本技术和 进行本PPG中描述的所有四个项目所需的专业知识，并将有助于确保可重现 结果是从项目到项目获得的。 相关性（请参阅说明）：