Biofilm Growth and Analysis Core

直到最近人们才认识到，细菌，包括葡萄球菌，常常以固着状态存在。 生物，附着在表面的高度复杂和有组织的群落，称为生物膜。这些 团头菌被包裹在由多糖、蛋白质和/或基因组DNA组成的基质中，并 暴露在各种不同的环境条件下。鉴于对细菌生物膜的研究仍处于起步阶段 相对较新的技术，特别是当考虑葡萄球菌生物膜时，存在着广泛的技术。 以及发展和分析这些复杂结构的策略。不足为奇的是，这种可变性使它 难以将实验结果与实验结果进行比较，并阻碍了对生物膜的理解 生物学。为了为生物膜的生长和分析提供统一和一致的策略，我们提出了一个 将为本次会议的参与者提供通用的生物膜生长设备和分析策略的核心 PPG。这个核心的具体目标1将是容纳和维护生物膜生长所需的设备 并将包括购买和维护将被所有人广泛使用的生物膜生长系统 本次PPG涉及四个项目。此外，它还将维护一个厌氧室，供项目1使用 和2.特定目标将制定和维护生物膜生长的标准化方案，并将为 为所有人提供产生一致和可重复的生物膜结果所需的方案和专业知识 四个项目。最后，具体目标3将是获得分析和分析所需的技术 生物膜对宿主的影响。这一目标的目标将是提供一种微探针检测系统 用来检测在生物膜形成过程中产生的特定代谢产物的波动，以及 Quanfit-Five Real-FIME聚合酶链式反应系统用于测量基因表达的变化。它还将提供Luminex 多分析检测系统跟踪多种炎症介质的产生 葡萄球菌感染。这些目标结合在一起，将使基本技术和 执行本PPG中描述的所有四个项目所需的专业知识，并将有助于确保可重现性 结果是从一个项目到另一个项目。 相关性(请参阅说明)：

It has only recently been recognized that bacteria, including the staphylococci, often exist as sessile organisms, attached to surfaces as highly complex and organized communities termed biofilms. These communifies are encased in matrices comprised of polysaccharides, proteins, and/or genomic DNA and are exposed to a remarkable variety of environmental conditions. Given that the study of bacterial biofilm is still relatively new, especially when considering staphylococcal biofllm, there exists a wide range of techniques and strategies to grow and analyze these complex structures. Not surprisingly, this variability makes it difficult to compare results from experiment to experiment and inhibits progress in understanding biofllm biology. To provide uniform and consistent strategies for the growth and analysis of biofilm, we propose a Core that will provide common biofllm growth equipment and analytical strategies for the participants of this PPG. Specific Aim 1 of this Core will be to house and maintain equipment needed for biofilm growth and will include the purchase and maintenance of biofllm growth systems that will be extensively used by all four projects involved in this PPG. In addition, it will also maintain an anaerobic chamber for use in Projects 1 and 2. Specific Aim will develop and maintain standardized protocols for biofilm growth and will serve to provide the protocols and expertise needed to produce consistent and reproducible biofllm results for all four Projects. Finally, Specific Aim 3 will be to acquire the technology needed for the analysis and impact of biofilm on the host. The goals of this aim will be to provide a microprobe detection system needed to detect fluctuations in speciflc metabolites produced during biofilm development, as well as a quanfitafive real-fime PCR system to measure changes in gene expression. It will also provide a Luminex Multi-analyte Detecfion system to follow the production of multiple inflammatory mediators during staphylococcal infection. Combined, these aims will make available the fundamental technology and expertise needed to carry-out all four projects described in this PPG and will help to ensure that reproducible results are obtained from project to project. RELEVANCE (See instructions):