Biofilm Growth and Analysis Core

生物膜生长和分析核心

• 批准号: 8496683
• 负责人: Jeffrey Lee Bose
• 金额: $ 12.63万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8496683
• 关键词: Animals; Bacteria; Biology; Communities; Complex; DNA; Detection; Development; Disease; Ensure; Equipment; Gene Expression; Genomics; Genus staphylococcus; Goals; Growth; Growth and Development function; Housing; Inflammation Mediators; Instruction; Liquid substance; Maintenance; Measures; Microbial Biofilms; Nutrient; Organism; Oxygen; Participant; Physiological; Polysaccharides; Production; Proteins; Protocols documentation; Staphylococcal Infections; Structure; Surface; Suspension substance; Suspensions; System; Techniques; Technology; Time; Waste Products; instrument; instrumentation; research study

It has only recently been recognized that bacteria, including the staphylococci, often exist as sessile organisms, attached to surfaces as highly complex and organized communities termed biofilms. These communifies are encased in matrices comprised of polysaccharides, proteins, and/or genomic DNA and are exposed to a remarkable variety of environmental conditions. Given that the study of bacterial biofilm is still relatively new, especially when considering staphylococcal biofllm, there exists a wide range of techniques and strategies to grow and analyze these complex structures. Not surprisingly, this variability makes it difficult to compare results from experiment to experiment and inhibits progress in understanding biofllm biology. To provide uniform and consistent strategies for the growth and analysis of biofilm, we propose a Core that will provide common biofllm growth equipment and analytical strategies for the participants of this PPG. Specific Aim 1 of this Core will be to house and maintain equipment needed for biofilm growth and will include the purchase and maintenance of biofllm growth systems that will be extensively used by all four projects involved in this PPG. In addition, it will also maintain an anaerobic chamber for use in Projects 1 and 2. Specific Aim will develop and maintain standardized protocols for biofilm growth and will serve to provide the protocols and expertise needed to produce consistent and reproducible biofllm results for all four Projects. Finally, Specific Aim 3 will be to acquire the technology needed for the analysis and impact of biofilm on the host. The goals of this aim will be to provide a microprobe detection system needed to detect fluctuations in speciflc metabolites produced during biofilm development, as well as a quanfitafive real-fime PCR system to measure changes in gene expression. It will also provide a Luminex Multi-analyte Detecfion system to follow the production of multiple inflammatory mediators during staphylococcal infection. Combined, these aims will make available the fundamental technology and expertise needed to carry-out all four projects described in this PPG and will help to ensure that reproducible results are obtained from project to project. RELEVANCE (See instructions):

直到最近才认识到，包括葡萄球菌在内的细菌通常以固着形式存在， 生物，附着在表面高度复杂和有组织的社区称为生物膜。这些 微生物被包裹在由多糖、蛋白质和/或基因组DNA组成的基质中， 暴露在各种各样的环境条件下鉴于细菌生物膜的研究仍在进行中， 相对较新，特别是当考虑葡萄球菌生物膜时，存在广泛的技术 以及培养和分析这些复杂结构的策略。毫不奇怪，这种可变性使其 难以比较实验与实验的结果，并且抑制了理解生物膜的进展 生物学为了给生物膜的生长和分析提供统一和一致的策略，我们提出了一个 核心，将提供共同的生物膜生长设备和分析战略的参与者， PPG。本核心的具体目标1是容纳和维护生物膜生长所需的设备 并将包括购买和维护生物膜生长系统， 这四个项目涉及到这个PPG。此外，该署亦会维持一个厌氧室，供工程项目1使用 和2.具体目标将开发和维护生物膜生长的标准化方案，并将服务于 为所有人提供产生一致和可重复的生物膜结果所需的方案和专业知识， 四个项目。最后，具体目标3将是获得分析所需的技术， 生物膜对宿主的影响。本课题的目标是提供一种微探针检测系统 需要检测生物膜发育过程中产生的特定代谢物的波动，以及 quanfitafive实时PCR系统来测量基因表达的变化。它还将提供Luminex 多分析物检测系统，用于跟踪在治疗期间多种炎症介质的产生 葡萄球菌感染结合起来，这些目标将提供基本的技术， 开展本PPG中描述的所有四个项目所需的专业知识，并将有助于确保 从一个项目到另一个项目都取得了成果。 相关性（参见说明）：