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Factors Determining Intraocular Pressure

决定眼压的因素

基本信息

批准号：
8035899
负责人：
SIMON W JOHN
金额：
$ 74.63万
依托单位：
JACKSON LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8035899
关键词：
9q22 Adopted Adult Age Age-Months Animal Model Atrophic Binding Biochemical Biological Models Blindness CYP1B1 gene Cell Death Chemicals Chromosome Mapping Chromosomes, Human, Pair 1 Clinical Cloning Collection Communities Complement Complex Data Disease Distal Drainage procedure Ethylnitrosourea Etiology Extracellular Matrix Family Funding Gene Expression Gene Targeting Genes Genetic Genome Genomics Glaucoma Goals Grant Hand Homozygote Human Human Genome Human Identifications Hydrophthalmos Individual Induced Mutation Inherited Investigation Knowledge Link Maps Metabolism Methodology Methods Modeling Molecular Molecular Genetics Mouse Strains Mus Mutagenesis Mutant Strains Mice Mutation Mutation Detection Nail-Patella Syndrome Open-Angle Glaucoma Optic Nerve Pathogenesis Pathologic Pathway interactions Patients Phenotype Physiologic Intraocular Pressure Point Mutation Population Predisposition Public Health Reporting Research Resistance Resources Retinal Ganglion Cells Risk Factors Screening procedure Structure Susceptibility Gene Syntenic Conservation System Testing Time Visual anterior chamber base comparative genomics empowered gene cloning gene discovery gene function genome-wide high intraocular pressure high throughput screening homeodomain improved male mouse model mutant novel novel strategies null mutation offspring seal success tool transcription factor

项目摘要

DESCRIPTION (provided by applicant): Glaucoma is a leading cause of blindness. Elevated intraocular pressure (IOP) is a major risk factor for glaucoma. Our goal is to continue to identify and characterize genetic factors that contribute to elevated IOP and glaucoma. We are using the mouse as a model system to conduct a phenotype driven mutagenesis screen to identify novel mechanisms and pathways involved in glaucoma pathogenesis. The power of a phenotype driven screen is that there is no requisite, a priori information for factors or pathways involved. In our previous funding period, we have identified a number of glaucoma relevant mouse mutants (Grms) that show hallmarks of glaucoma including high IOP, retinal ganglion cell death and optic nerve atrophy. In this proposal, we will identify the causative mutations in a few of these glaucoma-relevant mutants that we have started to study. We will characterize the genetic pathways involved and carry out more detailed investigations into the pathogenesis. Very few mouse models of glaucoma exist and our mutants will be valuable both as models for understanding human glaucoma and for facilitating the identification of human mutations. Since the small number of available mutants severely limits mouse studies of glaucoma, we propose a new Cyp 1b1 -sensitized END screen for dominant glaucoma mutations. A number of reported human glaucoma loci have a dominant effect. Mutations in the human CYP1B1 gene predispose people to high IOP and glaucoma, a predisposition we have repeated in mice. We will screen 1000 G1 males/year for IOP elevation and other ocular abnormalities, ten times more families than was possible in the previous granting period. We anticipate producing 15-40 glaucoma- relevant mutants that will be made available to the glaucoma community as quickly as is feasible. We are confident that continuing to use the power of a phenotype-driven mutagenesis screen in parallel with our unique tools to examine ocular phenotypes will enable us to identify and characterize new genetic factors that contribute to elevated IOP and glaucoma. The new mutants have great potential to transform our understanding of glaucoma. Relevance to Public Health: Glaucoma is one of the leading causes of blindness. Over 20 regions of the human genome have been implicated in glaucoma but very few genes have been identified. Our proposal aims to produce mouse models of human glaucoma to be used as tools to understand the mechanisms of glaucoma, to help identify human glaucoma genes and to test new treatments.

描述(申请人提供)：青光眼是导致失明的主要原因。眼压升高是青光眼的主要危险因素。我们的目标是继续识别和描述导致高眼压和青光眼的遗传因素。我们正在使用小鼠作为模型系统来进行表型驱动的突变筛选，以确定参与青光眼发病的新机制和途径。表型驱动筛选的力量在于，对于所涉及的因子或途径，没有必要的、先验的信息。在我们之前的资助期间，我们已经确定了一些青光眼相关小鼠突变(GRM)，这些突变显示出青光眼的特征，包括高眼压、视网膜神经节细胞死亡和视神经萎缩。在这个提案中，我们将确定我们已经开始研究的几个与青光眼相关的突变。我们将确定所涉及的遗传途径，并对其发病机制进行更详细的研究。青光眼的小鼠模型很少，我们的突变体作为理解人类青光眼的模型和促进识别人类突变的模型都是有价值的。由于少数可用突变体严重限制了小鼠对青光眼的研究，我们提出了一种新的针对显性青光眼突变的Cyp 1b1敏化末端筛选。一些已报道的人类青光眼基因座具有显性效应。人类细胞色素P1B1基因的突变使人们容易患上高眼压和青光眼，我们在老鼠身上重复了这种倾向。我们将每年筛查1000名G1男性眼压升高和其他眼部异常，比前一次批准期间可能的家庭多10倍。我们预计将生产15-40个青光眼相关突变体，将尽快提供给青光眼社区。我们相信，继续使用表型驱动的突变筛查的力量，同时使用我们独特的工具来检查眼睛表型，将使我们能够识别和表征导致高眼压和青光眼的新遗传因素。新的突变体具有巨大的潜力来改变我们对青光眼的理解。与公共健康相关：青光眼是导致失明的主要原因之一。人类基因组中有20多个区域与青光眼有关，但确定的基因很少。我们的建议旨在制造人类青光眼的小鼠模型，用作了解青光眼发病机制的工具，帮助识别人类青光眼基因，并测试新的治疗方法。