Project 2: Integrin Signaling and Physical Forces

该项目在要解决的问题和许多工具方面都具有创新性， 被雇用。通过整合素依赖性粘附的机械转导是当前感兴趣的主要领域，但详细的分子机制知之甚少。与醚研究相比，我们提出在细胞骨架和受体动力学的高分辨率分析的背景下研究这些现象。这种结合是非常新颖的，并提供了更深入的分子理解的主要机会，本质上是一种动态现象。此外，许多技术创新将结合在这项研究中。 这些包括整联蛋白和细胞骨架蛋白的突变体的分析，最新技术，高分辨率EM技术，新材料，nevel生物传感器，以及nevel成像和数据分析方法。因此，该应用程序包含多个创新方面，远远超出了目前的工作。

This project is innovative in both the questions to be addressed and many of the tools to be employed. Mechanotransduction by integrin-dependent adhesions is a major area of current interest but detailed molecular mechanisms are pooriy understood. What stands out compared to ether studies is that we propose to study these phenomena in the context of high resolution analysis of cytoskeletal and receptor dynamics. This combination is highly nevel and offers major opportunities for deeper molecular understanding of what is, in essence, a dynamic phenomenon. Additionally, numerous technical innovations will be combined in this study. These include analysis of mutants of integrins and cytoskeletal proteins, a state of the art, high resolution EM technique, novel materials, nevel biosensors, and nevel imaging and data analysis methods. The application therefore contains mulfiple innovative aspects that take it far beyond current work.