DECREASE OF DCI AND DCI-IPG IN MUSCLE & OVARY IN PCOS & NORMAL WOMEN

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 研究与年龄和体重匹配的正常妇女相比，多囊卵巢综合征（PCOS）妇女肌肉和卵巢中DCI（D-手性肌醇）和DCI-IPG（肌醇磷酸聚糖）的组织含量是否降低。 PCOS是一种知之甚少的疾病，影响6 - 10%的育龄妇女。PCOS的特征是男性激素分泌过多和排卵不足，是美国不孕症的主要原因。患有多囊卵巢综合征的女性也有患2型糖尿病的高风险，可能是由于伴随该综合征的胰岛素抵抗。 本研究旨在确定与年龄和体重匹配的正常女性的肌肉和卵巢相比，PCOS女性的肌肉和卵巢中总DCI与MYO的组织比率以及DCI-IPG与MYO-IPG的比率是否降低。 将在综合临床研究中心举行三次研究会议。研究将在妇科手术后两周内进行。在第一天，将进行血压、身高、体重和身体测量。将记录病史和月经史。将进行尿妊娠试验。将向受试者提供一个尿液试剂盒，其中包含24小时尿液样本的说明。第二天早上将样本送回诊所。禁食将于晚上8点开始。DCI摄入量可以来自蔬菜和水果含量高的饮食。营养师将与受试者讨论均衡、混合良好的饮食，受试者必须在研究开始前至少3天遵循该饮食。 第2天，受试者将在禁食12小时后返回诊所。将通过肝素锁抽取血液，肝素锁是插入静脉的塑料导管，可定期采集血样，无需重复穿刺。口服葡萄糖耐量试验将在患者饮用葡萄糖的情况下进行，并在接下来的几个小时内进行血液检查。将进行经阴道超声检查，并指示在晚上8点开始另一次快速检查。 第三天，禁食12小时后，将进行胰岛素敏感性测试。该测试使用葡萄糖静脉输注，并通过肝素锁采集几份血样。在预定的手术时间，一个卵巢和从异常肌肉中取出的活检将用于本研究的测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An investigation of whether tissue contents of DCI (D-chiro-inositol) and DCI-IPG (inositolphosphoglycan) are decreased in muscle and ovary obtained from women with polycystic ovary syndrome (PCOS) as compared with age and weight-matched normal women. PCOS is a poorly understood disorder that affects 6-10% of women of reproductive age. PCOS is characterized by an excessive secretion of masculine hormones and lack of ovulation and is the leading cause of infertility in the United States. Women with PCOS are also at high risk for developing type 2 diabetes, presumably due to the insulin resistance that accompanies the syndrome. This study is designed to determine if the tissue ratio of total DCI to MYO and the ratio DCI-IPG to MYO-IPG in muscle and ovary of women with PCOS are decreased compared to those in the muscle and ovary of age and weight-matched normal women. There will be three study sessions taking place at the General Clinical Research Center. The study will take place within two weeks of gynecological surgery. On the first day, blood pressure, height, weight, and body measurements will be done. A medical and menstrual history will be taken. A urine pregnancy test will be done. A urine kit with directions for a 24 hour urine sample will be given to the subject. The sample will be returned to the clinic the next morning. A fast will begin at 8:00 pm. DCI intake can come from a diet high in vegetables and fruits. A dietician will discuss a balanced, well mixed diet with the subjects which they must follow for at least three days before the start of the study. On day two, the subjects will return to the clinic after a 12 hour fast. Blood will be drawn through a heparin lock which is a plastic catheter inserted into a vein which allows blood samples to be obtained periodically without repeated sticks. An oral glucose tolerance test will be done where the patient drinks glucose and blood tests are done over the next few hours. A transvaginal ultrasound will be done and instructions for another fast starting at 8:00 pm will be given. On the third day, after the 12 hour fast, an insulin sensitivity test will be done. This test uses an intravenous infusion of glucose and several blood samples are taken through the heparin lock. At the time of the scheduled surgery, one ovary and biopsies taken from the abnormal muscle will be used for testing in this research.