DECREASE OF DCI AND DCI-IPG IN MUSCLE & OVARY IN PCOS & NORMAL WOMEN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：探讨多囊卵巢综合征(PCOS)患者的肌肉和卵巢组织中DCI(D-chiro-inositol-inositol-inositol)和DCI-ipg(Inositolphoglycan)的含量是否低于年龄和体重匹配的正常妇女。 多囊卵巢综合征是一种知之甚少的疾病，影响6%-10%的育龄妇女。多囊卵巢综合征的特点是男性荷尔蒙分泌过多，排卵不足，是美国不孕不育的主要原因。患有多囊卵巢综合征的女性患2型糖尿病的风险也很高，可能是因为伴随着这种综合征的胰岛素抵抗。 本研究旨在确定PCOS患者肌肉和卵巢中总DCI/MYO的组织比率和DCI-IPG/MYO-IPG的比率是否低于年龄和体重匹配的正常妇女的肌肉和卵巢中的DCI/MYO-IPG。 将在综合临床研究中心举行三次学习会议。这项研究将在妇科手术后两周内进行。在第一天，将进行血压、身高、体重和身体测量。将进行内科和月经史检查。将进行尿液妊娠测试。将向受试者赠送带有24小时尿样说明的尿液试剂盒。第二天早上，样本将被送回诊所。禁食从晚上8点开始DCI的摄入量可能来自高蔬菜和水果的饮食。营养师将与受试者讨论均衡、合理的混合饮食，他们必须在研究开始前至少三天遵循这些要求。 在第二天，受试者将在禁食12小时后返回诊所。血液将通过肝素锁抽出，这是一种插入静脉的塑料导管，允许定期采集血液样本，而不需要重复抽血。口服葡萄糖耐量测试将在患者饮用葡萄糖的地方进行，并在接下来的几个小时内进行血液测试。将做经阴道超声检查，并指示晚上8点开始另一次禁食。 第三天，禁食12小时后，将进行胰岛素敏感性测试。这项测试使用静脉输注葡萄糖，并通过肝素锁采集几个血样。在预定的手术时间，一个卵巢和从异常肌肉中提取的活组织将用于这项研究的测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An investigation of whether tissue contents of DCI (D-chiro-inositol) and DCI-IPG (inositolphosphoglycan) are decreased in muscle and ovary obtained from women with polycystic ovary syndrome (PCOS) as compared with age and weight-matched normal women. PCOS is a poorly understood disorder that affects 6-10% of women of reproductive age. PCOS is characterized by an excessive secretion of masculine hormones and lack of ovulation and is the leading cause of infertility in the United States. Women with PCOS are also at high risk for developing type 2 diabetes, presumably due to the insulin resistance that accompanies the syndrome. This study is designed to determine if the tissue ratio of total DCI to MYO and the ratio DCI-IPG to MYO-IPG in muscle and ovary of women with PCOS are decreased compared to those in the muscle and ovary of age and weight-matched normal women. There will be three study sessions taking place at the General Clinical Research Center. The study will take place within two weeks of gynecological surgery. On the first day, blood pressure, height, weight, and body measurements will be done. A medical and menstrual history will be taken. A urine pregnancy test will be done. A urine kit with directions for a 24 hour urine sample will be given to the subject. The sample will be returned to the clinic the next morning. A fast will begin at 8:00 pm. DCI intake can come from a diet high in vegetables and fruits. A dietician will discuss a balanced, well mixed diet with the subjects which they must follow for at least three days before the start of the study. On day two, the subjects will return to the clinic after a 12 hour fast. Blood will be drawn through a heparin lock which is a plastic catheter inserted into a vein which allows blood samples to be obtained periodically without repeated sticks. An oral glucose tolerance test will be done where the patient drinks glucose and blood tests are done over the next few hours. A transvaginal ultrasound will be done and instructions for another fast starting at 8:00 pm will be given. On the third day, after the 12 hour fast, an insulin sensitivity test will be done. This test uses an intravenous infusion of glucose and several blood samples are taken through the heparin lock. At the time of the scheduled surgery, one ovary and biopsies taken from the abnormal muscle will be used for testing in this research.