COBRE: UNE MED CTR: CORE D: DNA MICROARRAY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 包括人类、老鼠和果蝇基因组在内的几种不同基因组测序的完成，为研究人员带来了巨大的机会 生物医学进展(沃特斯顿等人，2002年；文特尔等人，2001年)。DNA微阵列技术是利用序列数据的新技术之一，是一种强大的高通量手段，可以获得对正常和与疾病相关的生物过程的关键洞察(Holloway AJ等人，2002年；Shultz和Downdown，2001年)。虽然这项技术只在过去十年里才发展起来，但它发展迅速，使用微阵列的应用越来越多。微阵列实验能够全面评估全球基因表达谱的相对变化，因此可以用来阐明决定发育和对环境刺激的反应的转录机制(Lyer等人，1999)。它们也可以用来表征疾病状态，并将这些 转录图谱与预后(Chung等人，2002年)。除了设计用来评估转录活性的DNA微阵列外，还有一些DNA微阵列 旨在描述DNA/蛋白质相互作用以及遗传变异(Pollack和Lyer，2002；Patil N et al，2001)。据预测，在未来，DNA微阵列将用于 诊断的临床环境，或许还有个性化医学(Petricoin EF等人，2002年)。赔偿委员会DNA微阵列核心基金将通过为调查人员在各自的研究项目中利用DNA微阵列技术提供必要的资源，支持概述的Cobre项目。微阵列技术是一种手段，通过它可以获得急需的新技术 可以从生物学途径中获得洞察力，因为这将帮助初级调查人员获得数据，从而显著增加他们在申请方面具有竞争力的可能性。 校外资金。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The completion of the sequencing of several different genomes including the human, mouse, and drosophila genomes, has resulted in tremendous opportunities for researchers to make biomedical advances (Waterston et al, 2002; Venter et al, 2001). DNA microarray technology is one of the new technologies that takes advantage of the sequence data and is a powerful highthroughput means to gain crucial insight into both normal and disease-related biological processes (Holloway AJ et al, 2002; Shultz and Downward, 2001). Although the technology has only been developed in roughly the last ten years, it has advanced rapidly and the list of applications in which microarrays have been used is growing. Microarray experiments are capable of comprehensivelyassessing relative changes in global gene expression profiles and as such can be used to elucidate transcriptional mechanisms dictating development and the response to environmental stimuli (lyer et al, 1999). They can also be used to characterize disease states and associate these transcriptional profiles with prognostic outcome (Chung et al, 2002). In addition to DNA microarrays that are designed to assess transcriptional activity, there are DNA microarrays that are designed to characterize DNA / protein interactions as well as genetic variation (Pollack and lyer, 2002; Patil N et al, 2001). It is also predicted that in the future DNA microarrays will be used in clinical settings for diagnosis and perhaps personalized medicine (Petricoin EF et al, 2002). The UNMC DNA Microarray Core Facility will support the COBRE project as outlined by providing the necessary resources for the investigators to utilize DNA microarray technology in their respective research projects. Microarray technology is a means by which much needed novel insights can be gained in biological pathways, as such it will help the junior investigators obtain data to significantly increase the likelihood that they will be competitive in regard to applying for extramural funding.