UNMC: MICROARRAY CORE, GENOMICS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在INBRE资助的第一年，微阵列核心设施为INBRE同事以及内布拉斯加州的其他学术研究人员提供了服务和支持。资金用于购买制造和处理载玻片的试剂，升级对设施运行至关重要的微阵列特定仪器，并提供人员支持，包括为研究技术人员提供50%的FTE，为核心主任提供15%的FTE。从INBRE项目获得的支持对核心设施向学术界提供功能基因组学支持的能力产生了巨大的影响。以下是自2004年6月以来核心所取得的成就。微阵列服务和支持第4年INBRE Associates: Kim Carlson博士内布拉斯加州大学科尔尼分校：该设施已经完成了一套微阵列实验（分析正在进行中）与Dr. Carlson使用果蝇Affymetrix基因芯片进行功能基因组学实验。具体项目是利用果蝇作为模型系统，确定OTK18过表达对发育和免疫基因的基因调控。道格·克里斯滕森博士-韦恩州立大学：该机构已经打印了一个定制的斑点微阵列来调查单核细胞增生李斯特菌的基因表达。基因表达实验即将开始。这些数据将用于进一步了解与李斯特菌相关的毒力因素，以及人类免疫系统细胞如何刺激或不刺激这种病原体的独特基因转录。芭芭拉·克莱门特博士-多恩学院：核心设施一直在与克莱门特博士一起设计实验，研究膜渗透性对铜绿假单胞菌检测群体感应（QS）信号（n -酰基高丝氨酸内酯或AHL）能力的影响。实验正在进行中。加里·邓肯博士内布拉斯加州卫斯理大学：该机构一直在与邓肯博士合作，设计一种定制的斑点微阵列来调查滑囊草履虫小球藻病毒-1 （PBCV-1）基因表达。初步打印和随后的基因表达数据是有趣的。该病毒是一种具有一些非典型分子机制的古老病毒，因此可能有助于了解代谢和其他生物过程的进化。多恩学院博士凯瑟琳·塔尔曼：该机构一直在与塔尔曼博士合作设计实验，研究乙醇暴露后小鼠基因表达的变化。这是为了作为胎儿酒精综合征研究的一个模型。非inbre研究者：斑点阵列平台：核心常规打印和处理10K载玻片组，代表人类，小鼠和大鼠基因组，使用用BRIN资金购买的寡核苷酸组和机器人观测仪。Core还为研究人员打印定制阵列。除了上面列出的INBRE用户外，该设施还为来自内布拉斯加州四个机构的20名调查人员提供服务，其中15名研究人员在UNMC， 3名在UNL， 1名在UNO， 1名在Creighton大学。Affymetrix平台：由BRIN支持的实验室研究技术人员为内布拉斯加州三个机构的21名研究人员处理Affymetrix芯片。其中包括UNMC的19名调查员，UNO的1名调查员和Boystown国家研究医院的1名调查员。以上研究者研究领域的例子：视网膜疾病，前列腺癌，淋巴瘤，帕金森？S疾病、甲基化模式和癌症、相关神经变性、细菌感染相关疾病、白血病、肺病、发育性心脏缺陷、心力衰竭、青光眼、口腔癌、遗传性听力损失、垂体肿瘤、乳腺癌和糖尿病。设备采购32年# 4的资金被用来购买新打印机针机器监视人。这是必要的,保持监视人打印幻灯片与统一的斑点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray Core Facility has provided service and support to INBRE associates as well as other academic investigators in the State of Nebraska during Year #1 of the INBRE grant. Funds were used to purchase reagents to fabricate and process slides, upgrade microarray specific instrumentation critical to the operations of the facility, and to provide personnel support including 50 % FTE for a research technologist and 15% FTE for the director of the core. The support received from the INBRE program has had a tremendous impact on the ability of the core facility to provide functional genomics support to the academic community. Outlined below are the accomplishments of the core since June of 2004. MICROARRAY SERVICES AND SUPPORT YEAR #4 INBRE Associates: Kim Carlson Ph.D. University of Nebraska at Kearney: The facility has completed a set of microarray experiments (analysis underway) with Dr. Carlson to perform a functional genomics experiment using Drosophila Affymetrix GeneChips. The specific project is determining the gene regulation of developmental and immune genes as a result of OTK18 overexpression using Drosophila as a model system. Doug Christensen PhD-Wayne State: The facility has printed a custom spotted microarray to survey Listeria monocytogenes gene expression. Gene expression experiments will begin soon. This data will be used to further knowledge of virulence factors associated with Listeria and how cells of the human immune system may or may not stimulate unique gene transcription in this pathogen. Barbara Clement PhD-Doane College: The core facility has been working with Dr. Clement designing experiments to study the effect of membrane permeability on the ability of Pseudomonas aeruginosa to detect quorum sensing (QS) signals (N-acyl homoserine lactones or AHL). Experiments are underway. Garry Duncan PhD Nebraska Wesleyan University: The facility has been working with Dr. Duncan designing a custom spotted microarray to survey Paramecium bursaria Chlorella virus-1 (PBCV-1) gene expression. Preliminary printing and subsequent gene expression data is intriguing. This virus is an ancient virus with some atypical molecular mechanisms and thus may yield insight into evolution of metabolism and other biological processes. Kathleen Tallman PhD-Doane College: The facility has been working with Dr. Tallman designing experiments to study changes in gene expression in mice following ethanol exposure. This is intended to serve as a model for fetal alcohol syndrome studies. Non-INBRE Investigators: Spotted Array Platform: The core routinely prints and processes 10K slide sets representing human, mouse and rat genomes using the oligonucleotide sets and robotic spotter purchased with BRIN funds. The Core also prints custom arrays for researchers. In addition to the INBRE users listed above, the facility serviced 20 investigators from four institutions in the State of Nebraska. 15 of these researchers were at UNMC, 3 were at UNL, 1 at UNO, and 1 at Creighton University. Affymetrix Platform: The BRIN supported research technologist in the laboratory processed Affymetrix chips for 21 investigators located at three institutions in Nebraska. These include 19 investigators at UNMC, 1 at UNO, and 1 at Boystown National Research Hosptital. Examples of Research Areas Of Above Investigators: Retinal disease, prostate cancer, lymphoma, Parkinson?s disease, methlyation patterns and cancer, associated neural degeneration, bacterial infection associated disease, leukemia, pulmonary disease, developmental heart defects, heart failure, glaucoma, oral cancer, genetics of hearing loss, pituitary tumors, breast cancer, and diabetes. EQUIPMENT PURCHASES Funds from year #4 were used to purchase 32 new printer pins for the robotic spotter. This is necessary to keep the spotter printing slides with uniform spots.