UNMC: MICROARRAY CORE, GENOMICS

UNMC：微阵列核心、基因组学

• 批准号: 7381506
• 负责人: JAMES D EUDY
• 金额: $ 13.01万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381506
• 关键词: UNMC; MICROARRAY; CORE; GENOMICS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray Core Facility has provided service and support to INBRE associates as well as other academic investigators in the State of Nebraska during Year #1 of the INBRE grant. Funds were used to purchase reagents to fabricate and process slides, upgrade microarray specific instrumentation critical to the operations of the facility, and to provide personnel support including 50 % FTE for a research technologist and 15% FTE for the director of the core. The support received from the INBRE program has had a tremendous impact on the ability of the core facility to provide functional genomics support to the academic community. Outlined below are the accomplishments of the core since June of 2004. MICROARRAY SERVICES AND SUPPORT YEAR #4 INBRE Associates: Kim Carlson Ph.D. University of Nebraska at Kearney: The facility has completed a set of microarray experiments (analysis underway) with Dr. Carlson to perform a functional genomics experiment using Drosophila Affymetrix GeneChips. The specific project is determining the gene regulation of developmental and immune genes as a result of OTK18 overexpression using Drosophila as a model system. Doug Christensen PhD-Wayne State: The facility has printed a custom spotted microarray to survey Listeria monocytogenes gene expression. Gene expression experiments will begin soon. This data will be used to further knowledge of virulence factors associated with Listeria and how cells of the human immune system may or may not stimulate unique gene transcription in this pathogen. Barbara Clement PhD-Doane College: The core facility has been working with Dr. Clement designing experiments to study the effect of membrane permeability on the ability of Pseudomonas aeruginosa to detect quorum sensing (QS) signals (N-acyl homoserine lactones or AHL). Experiments are underway. Garry Duncan PhD Nebraska Wesleyan University: The facility has been working with Dr. Duncan designing a custom spotted microarray to survey Paramecium bursaria Chlorella virus-1 (PBCV-1) gene expression. Preliminary printing and subsequent gene expression data is intriguing. This virus is an ancient virus with some atypical molecular mechanisms and thus may yield insight into evolution of metabolism and other biological processes. Kathleen Tallman PhD-Doane College: The facility has been working with Dr. Tallman designing experiments to study changes in gene expression in mice following ethanol exposure. This is intended to serve as a model for fetal alcohol syndrome studies. Non-INBRE Investigators: Spotted Array Platform: The core routinely prints and processes 10K slide sets representing human, mouse and rat genomes using the oligonucleotide sets and robotic spotter purchased with BRIN funds. The Core also prints custom arrays for researchers. In addition to the INBRE users listed above, the facility serviced 20 investigators from four institutions in the State of Nebraska. 15 of these researchers were at UNMC, 3 were at UNL, 1 at UNO, and 1 at Creighton University. Affymetrix Platform: The BRIN supported research technologist in the laboratory processed Affymetrix chips for 21 investigators located at three institutions in Nebraska. These include 19 investigators at UNMC, 1 at UNO, and 1 at Boystown National Research Hosptital. Examples of Research Areas Of Above Investigators: Retinal disease, prostate cancer, lymphoma, Parkinson?s disease, methlyation patterns and cancer, associated neural degeneration, bacterial infection associated disease, leukemia, pulmonary disease, developmental heart defects, heart failure, glaucoma, oral cancer, genetics of hearing loss, pituitary tumors, breast cancer, and diabetes. EQUIPMENT PURCHASES Funds from year #4 were used to purchase 32 new printer pins for the robotic spotter. This is necessary to keep the spotter printing slides with uniform spots.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。微阵列核心设施在INBRE资助的第一年为INBRE的同事以及内布拉斯加州的其他学术研究人员提供服务和支持。资金用于购买试剂以制作和处理载玻片，升级对设施运营至关重要的微阵列专用仪器，并提供人员支持，包括研究技术人员的50% FTE和核心主任的15% FTE。从INBRE计划获得的支持对核心设施向学术界提供功能基因组学支持的能力产生了巨大影响。下文概述了核心小组自2004年6月以来取得的成绩。 微阵列服务和支持第4年INBRE合伙人：Kim Carlson博士内布拉斯加大学科尔尼分校：该设施已经完成了一系列微阵列实验（分析正在进行中）与卡尔森博士进行功能基因组学实验使用果蝇Affyphilus基因芯片。具体的项目是使用果蝇作为模型系统，确定OTK 18过表达导致的发育和免疫基因的基因调控。 道格克里斯滕森博士韦恩州：该设施已打印一个定制的斑点微阵列调查李斯特菌基因表达。基因表达实验开始不久。这些数据将用于进一步了解与李斯特菌相关的毒力因子，以及人类免疫系统细胞如何刺激这种病原体的独特基因转录。 Barbara Clement博士-Doane学院：核心设施一直与Clement博士合作设计实验，研究膜通透性对铜绿假单胞菌检测群体感应（QS）信号（N-酰基高丝氨酸内酯或阿勒）能力的影响。实验正在进行中。 内布拉斯加卫斯理大学加里邓肯博士：该设施一直与邓肯博士合作，设计一个定制的斑点微阵列，以调查草履虫小球藻病毒-1（PBCV-1）基因表达。初步打印和随后的基因表达数据是有趣的。这种病毒是一种古老的病毒，具有一些非典型的分子机制，因此可能有助于了解代谢和其他生物过程的进化。 Kathleen Tallman博士-Doane学院：该机构一直与Tallman博士合作设计实验，研究小鼠暴露于乙醇后基因表达的变化。这是为了作为胎儿酒精综合征研究的模型。 非INBRE研究者：点阵平台：该核心使用BRIN基金购买的寡核苷酸组和机器人点样器，定期打印和处理代表人类、小鼠和大鼠基因组的10 K载玻片组。Core还为研究人员打印定制数组。除了上述INBRE用户外，该设施还为内布拉斯加州四个机构的20名调查员提供服务。这些研究人员中有15人在UNMC，3人在UNL，1人在UNO，1人在Creighton大学。 Affyoung平台：BRIN支持实验室的研究技术专家为内布拉斯加州三个机构的21名研究人员处理Affymetrix芯片。其中包括联合国监测委员会的19名调查员、联合国办事处的1名调查员和Boystown国家研究医院的1名调查员。 上述研究者的研究领域示例：视网膜疾病、前列腺癌、淋巴瘤、帕金森？s疾病、甲基化模式和癌症、相关神经变性、细菌感染相关疾病、白血病、肺病、发育性心脏缺陷、心力衰竭、青光眼、口腔癌、听力损失遗传学、垂体瘤、乳腺癌和糖尿病。 设备采购第4年的资金用于为自动点样器购买32个新的打印机针。这是必要的，以保持点样器打印具有均匀斑点的载玻片。