COBRE: UNE MED CTR: CORE D: DNA MICROARRAY CORE

COBRE：UNE MED CTR：核心 D：DNA 微阵列核心

• 批准号: 7382090
• 负责人: JAMES D EUDY
• 金额: $ 9.36万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382090
• 关键词: COBRE; UNE; MED; CTR; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The completion of the sequencing of several different genomes including the human, mouse, and drosophila genomes, has resulted in tremendous opportunities for researchers to make biomedical advances (Waterston et al, 2002; Venter et al, 2001). DNA microarray technology is one of the new technologies that takes advantage of the sequence data and is a powerful highthroughput means to gain crucial insight into both normal and disease-related biological processes (Holloway AJ et al, 2002; Shultz and Downward, 2001). Although the technology has only been developed in roughly the last ten years, it has advanced rapidly and the list of applications in which microarrays have been used is growing. Microarray experiments are capable of comprehensively assessing relative changes in global gene expression profiles and as such can be used to elucidate transcriptional mechanisms dictating development and the response to environmental stimuli (lyer et al, 1999). They can also be used to characterize disease states and associate these transcriptional profiles with prognostic outcome (Chung et al, 2002). In addition to DNA microarrays that are designed to assess transcriptional activity, there are DNA microarrays that are designed to characterize DNA / protein interactions as well as genetic variation (Pollack and lyer, 2002; Patil N et al, 2001). It is also predicted that in the future DNA microarrays will be used in clinical settings for diagnosis and perhaps personalized medicine (Petricoin EF et al, 2002). The UNMC DNA Microarray Core Facility will support the COBRE project as outlined by providing the necessary resources for the investigators to utilize DNA microarray technology in their respective research projects. Microarray technology is a means by which much needed novel insights can be gained in biological pathways, as such it will help the junior investigators obtain data to significantly increase the likelihood that they will be competitive in regard to applying for extramural funding.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。包括人类、小鼠和果蝇基因组在内的几种不同基因组测序的完成，为研究人员取得生物医学进步带来了巨大的机会（Waterston et al ., 2002; Venter et al ., 2001）。DNA微阵列技术是利用序列数据的新技术之一，是一种强大的高通量手段，可以深入了解正常和疾病相关的生物过程（Holloway AJ et al ., 2002; Shultz and down, 2001）。虽然这项技术在过去大约十年里才发展起来，但它发展迅速，微阵列的应用范围也在不断扩大。微阵列实验能够全面评估全球基因表达谱的相对变化，因此可以用来阐明决定发育和对环境刺激的反应的转录机制（lyer等，1999）。它们还可用于表征疾病状态，并将这些转录谱与预后结果联系起来（Chung et al ., 2002）。除了设计用于评估转录活性的DNA微阵列外，还有设计用于表征DNA /蛋白质相互作用以及遗传变异的DNA微阵列（Pollack和lyer, 2002； Patil N等人，2001）。据预测，在未来，DNA微阵列将用于临床诊断和个性化医疗（Petricoin et al, 2002）。UNMC DNA微阵列核心设施将支持COBRE项目，为研究人员在各自的研究项目中利用DNA微阵列技术提供必要的资源。微阵列技术是一种手段，通过它可以获得生物学途径中急需的新见解，因此它将帮助初级研究人员获得数据，从而大大增加他们在申请校外资金方面的竞争力。