UNMC: MICROARRAY CORE, GENOMICS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 联合国医学中心DNA微阵列核心设施将支持InBRE项目，为研究人员在各自的研究项目中利用DNA微阵列技术提供必要的资源。DNA微阵列技术是研究生物系统的重要工具。其用于多种生物学研究应用，包括全基因组转录测量、全基因组基因分型和染色体拷贝数测量（斯托顿，2005;托德等人，2007）。微阵列还用于表观遗传学研究，以监测甲基化状态以及DNA /蛋白质相互作用，如转录因子结合位点和组蛋白乙酰化（Hoheisel，2006）。在比较基因组杂交（CGH）背景下，DNA微阵列也已成为临床遗传学环境中的常规诊断工具，并用于鉴定导致各种发育缺陷的亚显微缺失和扩增（Oostlander et al，2004）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The UNMC DNA Microarray Core Facility will support the InBRE project by providing the necessary resources for investigators to utilize DNA microarray technology in their respective research projects. DNA microarray technology is an important tool used in the investigation of biological systems. It is used in a variety of biological research applications including genome-wide transcription measurements, genome-wide genotyping and chromosome copy number measurements (Stoughton, 2005; Todd et al 2007). Microarrays are also used in epigenetic studies to monitor methylation status as well as DNA / protein interactions such as transcription factor binding sites and histone acetylation (Hoheisel, 2006). DNA microarrays have also become a routine diagnostic tool in clinical genetics settings in the context of comparative genomic hybridization (cGH), and are used to identify submicroscopic deletions and amplifications responsible for a variety of developmental defects (Oostlander et al, 2004).