UNMC: MICROARRAY CORE, GENOMICS

UNMC：微阵列核心、基因组学

• 批准号: 8359991
• 负责人: JAMES D EUDY
• 金额: $ 6.87万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359991
• 关键词: Binding Sites; Chromosomes; Clinical; Core Facility; DNA Microarray Chip; DNA-Protein Interaction; Defect; Development; Diagnostic; Epigenetic Process; Funding; Genetic; Genetic Transcription; Genomics; Genotype; Grant; Histone Acetylation; Investigation; Measurement; Methylation; Microarray Analysis; Monitor; National Center for Research Resources; Nebraska; Principal Investigator; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Source; United States National Institutes of Health; biological research; biological systems; comparative genomic hybridization; cost; functional genomics; genome-wide; tool; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The UNMC DNA Microarray Core Facility will support the InBRE project by providing the necessary resources for investigators to utilize DNA microarray technology in their respective research projects. DNA microarray technology is an important tool used in the investigation of biological systems. It is used in a variety of biological research applications including genome-wide transcription measurements, genome-wide genotyping and chromosome copy number measurements (Stoughton, 2005; Todd et al 2007). Microarrays are also used in epigenetic studies to monitor methylation status as well as DNA / protein interactions such as transcription factor binding sites and histone acetylation (Hoheisel, 2006). DNA microarrays have also become a routine diagnostic tool in clinical genetics settings in the context of comparative genomic hybridization (cGH), and are used to identify submicroscopic deletions and amplifications responsible for a variety of developmental defects (Oostlander et al, 2004).

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 UNMC DNA 微阵列核心设施将通过为研究人员在各自的研究项目中利用 DNA 微阵列技术提供必要的资源来支持 InBRE 项目。 DNA微阵列技术是生物系统研究中使用的重要工具。它用于各种生物研究应用，包括全基因组转录测量、全基因组基因分型和染色体拷贝数测量（Stoughton，2005；Todd 等，2007）。微阵列还用于表观遗传学研究，以监测甲基化状态以及 DNA/蛋白质相互作用，例如转录因子结合位点和组蛋白乙酰化（Hoheisel，2006）。 DNA 微阵列也已成为比较基因组杂交 (cGH) 背景下临床遗传学环境中的常规诊断工具，并用于识别导致各种发育缺陷的亚显微缺失和扩增（Oostlander 等，2004）。