UNMC: MICROARRAY CORE, GENOMICS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 联合国医学中心DNA微阵列核心设施将支持InBRE项目，为研究人员在各自的研究项目中利用DNA微阵列技术提供必要的资源。DNA微阵列技术是研究生物系统的重要工具。其用于多种生物学研究应用，包括全基因组转录测量、全基因组基因分型和染色体拷贝数测量（斯托顿，2005;托德等人，2007）。微阵列还用于表观遗传学研究，以监测甲基化状态以及DNA /蛋白质相互作用，如转录因子结合位点和组蛋白乙酰化（Hoheisel，2006）。在比较基因组杂交（CGH）背景下，DNA微阵列也已成为临床遗传学环境中的常规诊断工具，并用于鉴定导致各种发育缺陷的亚显微缺失和扩增（Oostlander et al，2004）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The UNMC DNA Microarray Core Facility will support the InBRE project by providing the necessary resources for investigators to utilize DNA microarray technology in their respective research projects. DNA microarray technology is an important tool used in the investigation of biological systems. It is used in a variety of biological research applications including genome-wide transcription measurements, genome-wide genotyping and chromosome copy number measurements (Stoughton, 2005; Todd et al 2007). Microarrays are also used in epigenetic studies to monitor methylation status as well as DNA / protein interactions such as transcription factor binding sites and histone acetylation (Hoheisel, 2006). DNA microarrays have also become a routine diagnostic tool in clinical genetics settings in the context of comparative genomic hybridization (cGH), and are used to identify submicroscopic deletions and amplifications responsible for a variety of developmental defects (Oostlander et al, 2004).