INBRE: UOFL: GENOMICS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 共享的，集中的研究核心设施旨在为路易斯维尔大学及其KBRIN/INBRE附属机构提供测序和基因微阵列服务。 2009年9月，UofL微阵列设施搬到了新的临床转化研究大楼（CTRB），该大楼于2009年8月在同一个健康科学中心校区开业。该建筑为该设施提供了最先进的新的和扩展的实验室空间。新的设施已经接近。1500平方英尺，其中包括四个大型实验室区与工作台，一个单独的房间处理臭氧敏感安捷伦阵列和PCR前室。该设施包括Agilent微阵列扫描仪G2505 B、工作站和杂交烘箱G2545 A、Affypholic微阵列系统（包括Affypholic GCS 3000扫描仪7 G、两个射流站450和杂交烘箱640）、Beckman CEQ 8000测序仪、Agilent生物分析仪2000、Nanodrop ND-1000和三台Applied Biosystems PCR热循环仪9700。该中心持续举办专门培训。 最重要的是，此举允许将Affyoung和Agilent工厂的组件及其各自的人员合并到一个集中的工厂位置，为所有硬件和仪器提供充足的工作台空间，工作和存储空间以及所有人员的办公桌区域。 该机构正在获得CLIA认证，以便使用Agilent平台对临床患者样本进行标准化CGH基因检测。 随着所提供服务的扩展，引入了使用Agilent或Affytron阵列平台对人类和啮齿动物样品进行microRNA表达谱分析，并且现在常规进行。 另外，Yinlu Chen，M.S.，高级研究分析师，于2009年12月被聘用，作为工厂人员的补充，以支持现场客户数据分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The shared, centralized research core facility is designed to provide sequencing and gene-micro-array services to the University of Louisville and its KBRIN/INBRE affiliated institutions. In September 2009, the UofL Microarray Facility moved to the new Clinical Translational Research Building (CTRB), opened in August 2009 on the same Health Science Center Campus. This building provides state-of-the-art new and expanded lab space for the Facility. The new facility is approx. 1500 square feet, which includes four large lab areas with benches, a separate room for processing ozone sensitive Agilent arrays and a pre-PCR room. The facility houses an Agilent Microarray Scanner G2505B, Workstation, and Hybridization Oven G2545A, an Affymetrix Microarray System, including an Affymetrix GCS 3000 Scanner 7G, two Fluidics Stations 450 and a Hybridization Oven 640, a Beckman CEQ 8000 Sequencer, an Agilent Bioanalyzer 2000, a Nanodrop ND-1000 and three Applied Biosystems PCR Thermocyclers 9700. This core hosts specialized training on an on going basis. Most importantly, the move allowed to physically merge the Affymetrix and Agilent components of the Facility and their respective personnel into one centralized Facility location, with ample bench space for all hardware and instrumentation, work and storage space, and desk areas for all personnel. The Facility is in the process of obtaining CLIA certification for the processing of standardized CGH genetic tests on clinical patient samples using the Agilent platform. As expansion of the services offered, microRNA expression profiling on human and rodent samples using either the Agilent or Affymetrix array platforms was introduced and is now routinely performed. Also, Yinlu Chen, M.S., Sr. Research Analyst, was hired in Dec 2009 as addition to the Facility personnel to support client data analyses onsite.