INBRE: UOFL: GENOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 共享、集中的研究核心设施旨在为路易斯维尔大学及其KBRIN/INBRE附属机构提供测序和基因微阵列服务。 2009年9月，UofL微阵列设施搬到了新的临床翻译研究大楼(CTRB)，该大楼于2009年8月在同一健康科学中心园区开放。这座建筑为该设施提供了最先进的新的和扩大的实验室空间。新的设施大约是。1500平方英尺，其中包括四个带长凳的大型实验室区域，一个单独的处理臭氧敏感安捷伦阵列的房间和一个预聚合酶链式反应的房间。该工厂配备了一台安捷伦微阵列扫描仪G2505B、工作站和杂交炉G2545A，一套Affymetrix微阵列系统，包括一台Affymetrix GCS 3000扫描仪7G、两个流体站450和一个杂交烤箱640、Beckman CEQ 8000测序仪、安捷伦生物分析仪2000、NanoDrop ND-1000和三台应用生物系统PCR热循环仪9700。该中心经常举办专门的培训。 最重要的是，此举允许将设施的Affymetrix和Agilent组件及其各自的人员实际合并到一个集中的设施位置，为所有硬件和仪器、工作和存储空间以及所有人员的办公区提供充足的工作台空间。 该机构正在获得CLIA认证，以便使用安捷伦平台对临床患者样本进行标准化的CGH基因测试。 随着所提供服务的扩展，采用Agilent或Affymetrix阵列平台对人和啮齿动物样本进行microRNA表达谱分析，现在是常规操作。 此外，高级研究分析师陈银路于2009年12月受聘，作为设施人员的补充，在现场支持客户数据分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The shared, centralized research core facility is designed to provide sequencing and gene-micro-array services to the University of Louisville and its KBRIN/INBRE affiliated institutions. In September 2009, the UofL Microarray Facility moved to the new Clinical Translational Research Building (CTRB), opened in August 2009 on the same Health Science Center Campus. This building provides state-of-the-art new and expanded lab space for the Facility. The new facility is approx. 1500 square feet, which includes four large lab areas with benches, a separate room for processing ozone sensitive Agilent arrays and a pre-PCR room. The facility houses an Agilent Microarray Scanner G2505B, Workstation, and Hybridization Oven G2545A, an Affymetrix Microarray System, including an Affymetrix GCS 3000 Scanner 7G, two Fluidics Stations 450 and a Hybridization Oven 640, a Beckman CEQ 8000 Sequencer, an Agilent Bioanalyzer 2000, a Nanodrop ND-1000 and three Applied Biosystems PCR Thermocyclers 9700. This core hosts specialized training on an on going basis. Most importantly, the move allowed to physically merge the Affymetrix and Agilent components of the Facility and their respective personnel into one centralized Facility location, with ample bench space for all hardware and instrumentation, work and storage space, and desk areas for all personnel. The Facility is in the process of obtaining CLIA certification for the processing of standardized CGH genetic tests on clinical patient samples using the Agilent platform. As expansion of the services offered, microRNA expression profiling on human and rodent samples using either the Agilent or Affymetrix array platforms was introduced and is now routinely performed. Also, Yinlu Chen, M.S., Sr. Research Analyst, was hired in Dec 2009 as addition to the Facility personnel to support client data analyses onsite.