Imaging

现代细胞和分子生物学依赖于高分辨率的时空分布分析， 活体和固定标本中的结构和分子。在过去的9年里， 华盛顿大学（UW）的交流障碍研究人员的能力得到了极大的提高 NIDCD P30显微成像核心（Core C）。这些调查人员有着长期的活跃历史， 这些互动导致了许多共同撰写的出版物和合作赠款。核心C 通过增加研究人员获得现代成像方法的机会，促进了显微成像能力 获取和处理，扩大工具和实验方法的广度和效率， 数字成像，并增加其用户之间的科学互动。这个核心的主要设施， 数字显微镜中心位于人类发展和残疾中心（CHDD）， 毗邻弗吉尼亚州梅里尔布洛德尔听力研究中心（VMBHRC）。它包括5个显微镜， 成像工作站和先进的数据存档系统。设备、人员配备和日常工作 通过CHDD和VMBHRC之间的伙伴关系，该设施的运作是可能的， 证明非常成功。为核心用户提供的额外成像设施位于数字化 显微镜中心。 这一核心所作的最重要和最基本的贡献是为工作人员提供薪金支助 科学家Glen MacDonald，并为核心用户全额支付几台显微镜的使用费。先生 MacDonald拥有23年的荧光标记、显微成像和数字成像经验 处理.在过去的9年里，他管理着这个成像设施，升级了它的功能， 为研究人员、工作人员和学生提供培训。他有先进的标记和成像技术， 帮助购买和维护最先进的成像硬件和软件，并建立了严格的 数据备份系统。他的努力是无价的核心。此外，P30还承担了 显微镜的使用，否则将是非常繁重的个人研究计划。

Modern cellular and molecular biology relies on high-resolution analysis of spatiotemporal distributions of structures and molecules in live and fixed specimens. For the past 9 years, microscopic imaging for researchers in communications disorders at the University of Washington (UW) has been greatly enhanced by the NIDCD P30 Microscopic Imaging Core (Core C). These investigators have a long history of active interactions that have resulted in many co-authored publications and collaborative grants. Core C has facilitated microscopic imaging capabilities by increasing investigator access to modern methods for image acquisition and processing, expanding the breadth and efficiency of tools and experimental approaches for digital imaging, and increasing scientific interactions among its users. The major facility for this Core, the Digital Microscopy Center is based in the Center on Human Development and Disability (CHDD), in space adjacent to the Virginia Merrill Bloedel Hearing Research Center (VMBHRC). It includes 5 microscopes, 3 imaging workstations, and an advanced data archival system. The equipment, staffing, and day-to-day operation of this facility are possible through a partnership between the CHDD and the VMBHRC that has proven highly successful. Additional imaging facilities for core users are located outside the Digital Microscopy Center. The most significant and essential contributions made by this core are the salary support for the Staff Scientist, Glen MacDonald, and full coverage of usage fees for several microscopes for Core users. Mr. MacDonald has 23 years' of experience with fluorescent labeling, microscopic imaging, and digital image processing. For the last 9 years, he has managed this imaging facilities, upgraded its capabilities, and provided training for investigators, staff, and students. He has advanced labeling and imaging techniques, helped to purchase and maintain state-of-the-art imaging hardware and software, and established rigorous data backup systems. His efforts are invaluable to the core. In addition, the P30 has covered costs for microscope usage that would otherwise be highly taxing to individual research programs.