CELL TISSUE AND IMAGING CORE

细胞组织和成像核心

• 批准号: 7778740
• 负责人: Sarah F Hamm-Alvarez
• 金额: $ 28.82万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7778740
• 关键词: Agreement; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Charge; Collaborations; Endothelial Cells; Environment; Fostering; Funding; Grant; Growth; Hepatic Stellate Cell; Hepatocyte; Human; Image; Kupffer Cells; Liver; Mus; National Institute on Alcohol Abuse and Alcoholism; Productivity; Publications; Rattus; Research; Research Personnel; Resource Sharing; Services; Source; Surgeon; Techniques; Tissues; Yang; cost; improved; interdisciplinary collaboration; member; programs; repository

During the past funding period, the Cell Culture Core has continued to provide isolation of mouse and rat hepatocytes from normal and diseased livers, cell line banking, and distribution of human hepatocytes. A separately funded (through NIAAA beginning in mid-2001) Non-parenchymal Liver Cell Core provides freshly isolated and cultured normal or diseased rat Kupffer cells, hepatic stellate cells, and sinusoidal endothelial cells. Effective 9/1/04, the Cell Culture Core stopped preparing human hepatocytes but instead distributes human hepatocytes prepared by a commercial source (CellzDirect, Tucson, Az). This is because an agreement was made between the USC liver surgeons and CellzDirect, which supports the liver tissue repository at USC and provides human hepatocytes free of charge to Liver Center investigators. The Cell Culture Core has continued to provide a vital service to the Center members and has remained near steady state from the previoijs to the current grant cycle in terms of usage (number of preps, investigators and grants) and productivity (number of publications). Sixteen members and affiliated members used the Cell Culture Core from 1998 to 2003 with 47 publications. In the current cycle, 2003 to 2008, 18 full members used the Core and generated 50 publications. In particular, the Cell Culture Core has been instrumental in assisting young investigators in establishing their independent research programs (e.g. H.P. Yang, D. Han, C. Ji and Z.X. Liu).

在过去的资助期间，细胞培养核心继续提供小鼠和 来自正常和患病肝脏的大鼠肝细胞、细胞系库和人类肝细胞的分布。一个 单独资助（通过 NIAAA 从 2001 年中期开始） 非实质肝细胞核心提供新鲜 分离培养正常或患病大鼠库普弗细胞、肝星状细胞、肝窦内皮细胞 细胞。自 2004 年 9 月 1 日起，细胞培养核心停止制备人肝细胞，而是分配 由商业来源（CellzDirect，图森，亚利桑那州）制备的人肝细胞。这是因为一个 南加州大学肝脏外科医生与支持肝脏组织的 CellzDirect 达成了协议 南加州大学的储存库，并向肝脏中心研究人员免费提供人类肝细胞。 细胞培养核心继续为中心成员提供重要的服务，并已 从上一个到当前的赠款周期，在使用方面（准备次数、 研究人员和资助）和生产力（出版物数量）。十六名会员及附属会员 1998 年至 2003 年期间使用细胞培养核心，发表了 47 篇论文。在当前周期中，2003年至2008年，18个完整 成员使用了核心并生成了 50 篇出版物。特别是，细胞培养核心已 有助于帮助年轻研究人员建立他们的独立研究项目（例如 H.P. 杨，D. Han，C. Ji，Z.X。刘）。