Pathology Core

项目总结（见说明）： 构成此SPORE应用程序的各个研究项目需要采购，处理， 以及来自卵巢癌和良性卵巢疾病患者的组织病理学材料的分析。 这些研究项目需要冷冻和福尔马林固定的石蜡包埋的肿瘤样本， 正常组织拟议的病理学核心增强了已经建立的M。D.安德森癌症 中心妇科肿瘤库和PSO赞助的M。D.安德森癌症中心 组织储存库，支持数据库和内联网访问。核心通过以下方式提供组织采集 经验丰富的妇科病理学家，以确保高质量的组织，为研究者参与这一 SPORE以及其他SPORE的调查人员。病理学核心的目标是提供冷冻 组织，石蜡包埋组织，以及与特定需求相关的组织病理学专业知识。 研究项目包括这个SPORE提案。为了实现这一目标，病理学核心提出了 具体目标如下，自上次更新以来保持不变。目标1将保持冻结 以及卵巢癌、良性卵巢过程和正常卵巢的石蜡包埋组织库。 主要组织来源为提交至M病理科的手术和活检标本。 D.安德森癌症中心。目标2将为所有临床标本提供病理学检查， 在必要时提供组织病理学技术服务。此类技术服务 包括免疫组织化学、原位杂交、产生特异性组织微阵列载玻片、病理 小鼠肿瘤的评价和组织切片的显微切割。目标3将建立血液/尿液/腹水 来自接受卵巢癌和良性卵巢过程手术的患者的液体储存库。这些 液体将为推定的预后和诊断标志物的系统检测提供资源 来源于基于组织的表达阵列和CGH实验。目标4将创建和维护一个 Pathology Core收集的所有样本的关系型SPORE数据库。该数据库将 提供一个虚拟组织库，可与所有SPORE研究者电子共享。

PROJECT SUMMARY (See instructions): The individual research projects comprising this SPORE application require the procurement, processing, and analysis of histopathological material from patients with ovarian cancer and benign ovarian diseases. The research projects have needs for frozen and formalin-fixed, paraffin-embedded samples of tumor and normal tissue. The proposed Pathology Core augments the already established M. D. Anderson Cancer Center Gynecological Tumor Bank and the PSO sponsored M. D. Anderson Cancer Center Centralized Tissue Repository with supporting database and intranet access. The Core provides for tissue acquisition by experienced gynecological pathologists to assure high-quality tissues for the investigators participating in this SPORE as well as investigators of other SPOREs. The goal of the Pathology Core is to provide frozen tissue, paraffin-embedded tissue, and histopathological expertise related to the specific needs of the research projects comprising this SPORE proposal. To achieve this goal, the Pathology Core proposes the following Specific Aims, which remain unchanged since the previous renewal. Aim 1 will maintain a frozen and paraffin-embedded tissue repository of ovarian cancer, benign ovarian processes, and normal ovary. The primary tissue source will be operative and biopsy specimens submitted to the Dept of Pathology at M. D. Anderson Cancer Center. Aim 2 will provide pathological review for all clinical specimens utilized in the SPORE projects and to provide histopathological technical services as necessary. Such technical services include immunohistochemistry, in situ hybridization, creation of specific tissue microarray slides, pathological evaluation of mouse tumors, and microdissection of tissue sections. Aim 3 will establish a blood/urine/ascites fluid repository from patients undergoing surgery for ovarian cancer and benign ovarian processes. These fluids will provide the resources for the systemic testing of putative prognostic and diagnostic markers derived from tissue-based expression array and CGH experiments. Aim 4 will create and maintain a relational SPORE Database for all samples collected by the Pathology Core. This SPORE Database will provide for a virtual tissue repository that can be electronically shared with all SPORE investigators.