Pathology Core

项目总结(见说明)： 组成这一孢子申请的个别研究项目需要采购、处理、 对卵巢癌和良性卵巢疾病患者的组织病理学资料进行分析。 研究项目需要冷冻和福尔马林固定的石蜡包埋的肿瘤样本和 正常组织。拟议的病理学核心增强了已经建立的M.D.安德森癌症 中心妇科肿瘤银行和PSO赞助的M.D.安德森癌症中心 具有支持数据库和内部网访问的组织存储库。核心通过以下方式提供组织获取 经验丰富的妇科病理学家，确保参与调查的研究人员获得高质量的组织 孢子以及其他孢子的调查人员。病理核心的目标是提供冰冻的 组织、石蜡包埋组织和组织病理学专业知识与患者的特定需求有关 组成这个孢子提案的研究项目。为了实现这一目标，病理学核心建议 遵循自上次延长以来保持不变的具体目标。目标一号将保持冻结状态 以及卵巢癌、良性卵巢突起和正常卵巢的石蜡包埋组织储存库。 主要的组织来源是手术和活组织标本，提交给密歇根大学病理学系。 D.安德森癌症中心。AIM 2将为所有临床标本提供病理学审查 并在必要时提供组织病理学技术服务。这样的技术服务 包括免疫组织化学、原位杂交、创建特定组织微阵列载玻片、病理 小鼠肿瘤的评估和组织切片的显微解剖。目标3将建立血/尿/腹水 卵巢癌和良性卵巢突起手术患者的体液储存库。这些 体液将为推测的预后和诊断标记物的系统测试提供资源 来源于基于组织的表达阵列和CGH实验。目标4将创建和维护一个 病理核心收集的所有样本的相关孢子数据库。这个孢子数据库将 提供一个可与所有孢子调查人员电子共享的虚拟组织储存库。

PROJECT SUMMARY (See instructions): The individual research projects comprising this SPORE application require the procurement, processing, and analysis of histopathological material from patients with ovarian cancer and benign ovarian diseases. The research projects have needs for frozen and formalin-fixed, paraffin-embedded samples of tumor and normal tissue. The proposed Pathology Core augments the already established M. D. Anderson Cancer Center Gynecological Tumor Bank and the PSO sponsored M. D. Anderson Cancer Center Centralized Tissue Repository with supporting database and intranet access. The Core provides for tissue acquisition by experienced gynecological pathologists to assure high-quality tissues for the investigators participating in this SPORE as well as investigators of other SPOREs. The goal of the Pathology Core is to provide frozen tissue, paraffin-embedded tissue, and histopathological expertise related to the specific needs of the research projects comprising this SPORE proposal. To achieve this goal, the Pathology Core proposes the following Specific Aims, which remain unchanged since the previous renewal. Aim 1 will maintain a frozen and paraffin-embedded tissue repository of ovarian cancer, benign ovarian processes, and normal ovary. The primary tissue source will be operative and biopsy specimens submitted to the Dept of Pathology at M. D. Anderson Cancer Center. Aim 2 will provide pathological review for all clinical specimens utilized in the SPORE projects and to provide histopathological technical services as necessary. Such technical services include immunohistochemistry, in situ hybridization, creation of specific tissue microarray slides, pathological evaluation of mouse tumors, and microdissection of tissue sections. Aim 3 will establish a blood/urine/ascites fluid repository from patients undergoing surgery for ovarian cancer and benign ovarian processes. These fluids will provide the resources for the systemic testing of putative prognostic and diagnostic markers derived from tissue-based expression array and CGH experiments. Aim 4 will create and maintain a relational SPORE Database for all samples collected by the Pathology Core. This SPORE Database will provide for a virtual tissue repository that can be electronically shared with all SPORE investigators.