Pathology Core

组成卵巢癌孢子应用的个别研究项目需要 卵巢癌和卵巢癌患者的组织病理学材料的获取、处理和分析 良性卵巢疾病。研究项目需要冰冻和福尔马林固定，石蜡包埋 肿瘤和正常组织的样本。病理学的核心是对已经确立的 M.D.安德森癌症中心妇科肿瘤银行和P30赞助的M.D.安德森癌症 具有支持数据库和内部网访问的中心集中式组织存储库。核心提供 由经验丰富的妇科病理学家采集组织，以确保为患者提供高质量的组织 参与这一孢子的调查人员以及其他孢子的调查人员。 病理核心的目标是提供冰冻组织、石蜡包埋组织和组织病理学 与组成本孢子提案的研究项目的具体需求有关的专业知识。至 为了实现这一目标，病理学核心有以下具体目标。目标1是保持一个冷冻的和 石蜡包埋的卵巢癌、良性卵巢突起和正常卵巢的组织库。这个 主要的组织来源是提交给M.D.病理科的手术和活组织标本。 安德森癌症中心。此外，与杜克大学的分包合同还提供了额外的卵巢 组织，尤其是早期卵巢癌。目标2是为所有临床病例提供病理回顾 在孢子项目中使用标本，并在必要时提供组织病理学技术服务。 这些技术服务包括免疫组织化学、原位杂交、特定组织的创建 微阵列载玻片和组织切片的显微解剖。目标3是建立血/尿/腹水 卵巢癌和良性卵巢突起手术患者的资料库。这些液体 提供资源，用于系统测试假定的预后和诊断标记物 组织表达阵列和计算全息实验。目标4是创建和维护孢子数据库 所有在M.D.安德森癌症中心和杜克大学收集的样本。这个孢子数据库 是一个由所有孢子研究人员以电子方式共享的虚拟组织存储库。

The individual research projects that make up this Ovarian Cancer SPORE application require the procurement, processing, and analysis of histopathological material from patients with ovarian cancer and benign ovarian diseases. The research projects have needs for frozen and formalin-fixed, paraffinembedded samples of tumor and normal tissue. The Pathology Core augments the already established M.D. Anderson Cancer Center Gynecological Tumor Bank and the P30-sponsored M.D. Anderson Cancer Center Centralized Tissue Repository with supporting database and intranet access. The Core provides tissue acquisition by experienced gynecological pathologists to assure high-quality tissues for the investigators participating in this SPORE as well as investigators of other SPOREs. The goal of the Pathology Core is to provide frozen tissue, paraffin-embedded tissue, and histopathological expertise related to the specific needs for the research projects comprising this SPORE proposal. To achieve this goal, the Pathology Core has the following Specific Aims. Aim 1 is to maintain a frozen and paraffin-embedded tissue repository of ovarian cancer, benign ovarian processes, and normal ovary. The primary tissue source is operative and biopsy specimens submitted to the Department of Pathology at M.D. Anderson Cancer Center. In addition, a subcontract with Duke University provides additional ovarian tissues, particularly early stage ovarian cancers. Aim 2 is to provide pathological review for all clinical specimens utilized in the SPORE projects and to provide histopathological technical services as necessary. Such technical services include immunohistochemistry, in situ hybridization, creation of specific tissue microarray slides, and microdissection of tissue sections. Aim 3 is to establish a blood/urine/ascites fluid repository from patients undergoing surgery for ovarian cancer and benign ovarian processes. These fluids provide the resources for the systemic testing of putative prognostic and diagnostic markers derived from tissue-based expression array and CGH experiments. Aim 4 is to create and maintain a SPORE Database for all samples collected at both M.D. Anderson Cancer Center and Duke University. This SPORE Database is a virtual tissue repository that is electronically shared by all SPORE investigators.