SINGLE-SHOT METABOLIC IMAGING OF THE RAT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 代谢活性底物如13Cl-丙酮酸的超极化使得利用MRS和化学位移成像研究体内代谢的新方法成为可能。然而，信号放大的瞬变特性要求采用快速数据采样技术。这项工作的目的是通过使用欠采样的13C螺旋CSI(SpCSI)与高性能梯度插入物相结合，在大鼠体内实现单次代谢成像。 实现的序列由切片(Z)选择性激发和用于组合空间(Xy)-谱(F)编码的螺旋读出梯度组成。螺旋波形设计为视场为48？48mm2，标称为3？3-mm2的平面内分辨率。插入梯度在单次激发模式下允许298赫兹的频谱宽度(Sw)。激励后采集32个回波，总捕获时间(TACQ)小于120ms。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hyperpolarization of metabolically active substrates such as 13C1-pyruvate permits new approaches to the investiga¿tion of in vivo metabolism using MRS and chemical shift imaging. However, the transient nature of the signal amplification necessitates fast data sampling techniques. The aim of this work was to achieve single-shot metabolic imaging in the rat in vivo by using undersampled 13C spiral CSI (spCSI) in combination with a high-performance gradient insert. The implemented sequence consists of a slice(z)-selective excitation and a spiral readout gradient for combined spatial(xy)-spectral(f) encoding. The spiral waveforms were designed for a FOV of 48¿48 mm2 with a nominal 3¿3-mm2 in-plane resolution. The insert gradients allowed a spectral width (SW) of 298 Hz in single-shot mode. With 32 echoes acquired after excitation, the total acquisition time (Tacq) was less than 120 ms.