IN VIVO DTI OF RAT BRAIN ON A 3T MR SYSTEM W/INSERT GRADIENTS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。前言：物质依赖的动物模型有助于识别成瘾的易感性、发展和维持的生物学机制。与人类酒精成瘾特别相关的是脑白质，尸检研究表明，脑白质会受到髓鞘和细胞骨架的降解，这可能在戒酒后是可逆的。酒精依赖的体内人体扩散张量成像(DTI)研究提供了常规MRI体积测量无法检测到的脑白质微结构破坏的证据。在此，我们报道了在配备高强度插入梯度线圈的3T人体MRI系统上用于研究大鼠脑的体内回波平面(EP)扩散张量成像(DTI)协议的发展。方法和讨论：健康成年Wistar大鼠在临床GE 3T磁共振扫描仪上进行扫描，设备包括高强度插入梯度线圈(500mT/m，1800T/m/S)和定制的正交鸟笼线圈(？=44 mm)。成像采用EP DTI方案，分辨率为0.5 mm各向同性。对b值为1009 S/mm~2的图像，在6个非共线方向上进行扩散加权。分数各向异性(FA)图像具有足够的信噪比来检测大鼠的脑白质结构。在FA值与最大本征值的特征向量的主分量(x、y和z)相乘的图像中，可以识别纤维的主要取向。资金来源：年龄和酒精中毒的相互作用，Adolf Pfefferbaum，5/1/1997-6/30/2006？R01 AA012388？在酒精中毒中的体内扩散和光谱脑成像？，Adolf Pefferbaum，8/1/2000-6/30/2010？U01 AA013521？INIA：成像核心？，Adolf Pfefferbaum，9/27/2001-8/31/2006相关出版物《E.V.Sullivan，E.Adalsteinsson，R.Sood，D.Mayer，R.L.Bell，McBride，T.-K.，Li白头翁(A.Pfefferbaum)--酒精嗜好(P)大鼠的脑纵向MRI研究：成人脑发育，酒精临床。实验研究报告，在回顾中。A.Pfefferbaum，E.Adalsteinsson，R.Sood，D.Mayer，R.L.Bell，W.McBride，T.-K.Li，E.V.Sullivan？第二部分--嗜酒(P)大鼠的纵向脑MRI研究：自愿慢性饮酒的影响，酒精临床。实验研究报告，在回顾中。D.Mayer，E.Adalsteinsson，B.K.Rutt，N.Zahr，E.V.Sullivan，A.Pfefferbaum？in Vivo Differbaum Imaging in大鼠大脑用3T临床磁共振系统和高强度插入梯度线圈获得的活体扩散张量成像，美国医学会会刊，2006年，第14届年会，西雅图。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Animal models of substance dependence can facilitate the identification of biological mechanisms underlying the predisposition, development, and maintenance of addiction. Of particular relevance to human alcohol addiction is brain white matter, which postmortem study has shown is subject to myelin and cytoskeletal degradation that may be reversible with abstinence. In vivo human diffusion tensor imaging (DTI) studies of alcohol dependence provide evidence for microstructural disruption of white matter not detectible with bulk volume measures of conventional MRI. Here, we report on the development of an in vivo echo-planar (EP) diffusion tensor imaging (DTI) protocol on a 3T human MRI system equipped with a high-strength insert gradient coil for study of rat brain. Methods and Discussion: Healthy adult Wistar rats were scanned in a clinical GE 3T MR scanner equipped with a high-strength insert gradient coil (500mT/m, 1800T/m/s) and a custom quadrature birdcage coil (? = 44 mm). Imaging was accomplished with an EP DTI protocol for 0.5mm isotropic resolution. Diffusion weighting was applied for b-value=1009 s/mm2 in 6 noncollinear directions. Fractional anisotropy (FA) images had adequate signal-to-noise ratio to detect rat brain white matter structures. The primary orientation of the fibers could be identified in images where the FA value is multiplied by the principal component (x, y, and z) of the eigenvector of the largest eigenvalue. Funding Sources ¿ R37 AA005965, ¿CNS DEFICITS: INTERACTION OF AGE AND ALCOHOLISM¿, Adolf Pfefferbaum, 5/1/1997-6/30/2006 ¿ R01 AA012388, ¿In Vivo Diffusion and Spectroscopic Brain Imaging in Alcoholism¿, Adolf Pefferbaum, 8/1/2000-6/30/2010 ¿ U01 AA013521, ¿INIA: Imaging Core¿, Adolf Pfefferbaum, 9/27/2001-8/31/2006 Relevant publications ¿ E.V. Sullivan, E. Adalsteinsson, R. Sood, D. Mayer, R.L. Bell, W. McBride, T.-K. Li, A. Pfefferbaum ¿Part I--Longitudinal Brain MRI Study of the Alcohol-Preferring (P) Rat: Adult Brain Growth¿, Alcohol Clin. Exp. Res., in review. ¿ A. Pfefferbaum, E. Adalsteinsson, R. Sood, D. Mayer, R.L. Bell, W. McBride, T.-K. Li, E.V. Sullivan ¿Part II--Longitudinal Brain MRI Study of the Alcohol-Preferring (P) Rat: Effects of Voluntary Chronic Alcohol Consumption¿, Alcohol Clin. Exp. Res., in review. ¿ D. Mayer, E. Adalsteinsson, B.K. Rutt, N. Zahr, E.V. Sullivan, A. Pfefferbaum ¿In Vivo Diffusion Tensor Imaging of Rat Brain Acquired with a 3T Clinical MRI System and a High Strength Insert Gradient Coil¿, Proc ISMRM, 14th Annual Meeting, Seattle, 2006.