CONFORMATIONAL STUDY OF THE SACCHAROMYCES CEREVISIAE SEPTIN OCTAMERIC COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 隔膜蛋白组装成细胞骨架聚合物。胞质分裂是细胞分裂的最后一步，细胞骨架是胞质分裂所必需的。在芽殖酵母中，在芽颈处可见丝状的隔蛋白环，并将其压缩以分离母细胞和子细胞。我们使用的是在体外自我组装的酵母细胞隔膜。有丝分裂的酿酒酵母细胞表达五种septins：Cdc 3，Cdc 10，Cdc 11，Cdc 12和Shs 1/Sep 7。除了Shs 1之外，所有这些都是细胞分裂所必需的。我们已经确定了四个基本septins的分子组织内的最小复杂的标记技术，使用电子显微镜。除了Cdc 10之外，所有的隔蛋白都具有预测的卷曲螺旋，其促进隔蛋白组装成成对的细丝。螺旋线圈在复合体中的定位仍有待确定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Septin proteins assemble into a cytoskeletal polymer. Septin cytoskeleton is essential for cytokinesis, the last step in cell division. In budding yeast, filamentous rings of septins have been visualized at the bud neck and constrict it in order to separate the mother and daughter cells. We are using yeast septins that self-assemble in vitro. Mitotic Saccharomyces cerevisiae cells express five septins: Cdc3, Cdc10, Cdc11, Cdc12, and Shs1/Sep7. All, but Shs1, are essential for cell division. We have determined the molecular organization of the four essential septins within the minimal complex by labeling techniques using electron microscopy. All of the septins but Cdc10 have a predicted coiled coil which promotes septin assembly into paired filaments. The positioning of the coiled coils into the complex is still to be determined.