SUBMITTED SAMPLES FOR THE GREAT LAKES BIOENERGY CENTER

为五大湖生物能源中心提交的样品

• 批准号: 8168957
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 2.41万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168957
• 关键词: Biomass; Carbohydrates; Carbon; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Computer software; Databases; Enzymes; Ethanol; Fossil Fuels; Funding; Goals; Grant; Hydrolysis; Institution; Modeling; Monitor; Plants; Process; Production; Research; Research Personnel; Resources; Sampling; Solutions; Source; Techniques; United States National Institutes of Health; base; depolymerization; enzyme pathway; improved; lignocellulose; metabolomics; monomer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To increase the contribution of biofuels to the US energy portfolio, the Great Lakes Bioenergy Center (GLBRC) will conduct research to remove bottlenecks in the biofuel pipeline. This process involves improving characteristics of biomass plants, processing of plant biomass and improving how we convert biomass into energy products. In part to achieve these goals, the GLBRC will deploy high-throughput NMR based metabolomics to help develop models for relevant enzymes, pathways and networks that may be involved in biofuel production. Unlike fossil fuel, biofuel is produced mostly from photosynthetic plants, therefore it is a carbon neutral and sustainable energy form. How to efficiently breakdown lignocelluloses into fermentable carbohydrate monomers and oligomers is the bottleneck of economically producing cellulosic ethanol or other bioenergy products. We apply solution NMR to various switchgrass and their different fraction to monitor biomass content changes. Meanwhile, enzymatic hydrolysis is an efficient technique for depolymerization of plant material. We present a model of predicting the enzyme products of cornstalk by employing various 1D and 2D NMR combining with our MMCD database and R-NMR software techniques.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了增加生物燃料对美国能源组合的贡献，五大湖生物能源中心(GLBRC)将进行研究，以消除生物燃料管道中的瓶颈。这一过程包括改善生物质植物的特性，加工植物生物质，以及改进我们将生物质转化为能源产品的方式。在一定程度上为了实现这些目标，GLBRC将部署基于高通量核磁共振的代谢组学，以帮助开发可能涉及生物燃料生产的相关酶、途径和网络的模型。 与化石燃料不同，生物燃料主要来自光合作用的植物，因此它是一种碳中性和可持续的能源形式。如何有效地将木质纤维素分解成可发酵的碳水化合物单体和低聚物是经济生产纤维素乙醇或其他生物能源产品的瓶颈。我们应用溶液核磁共振技术对不同枝条及其不同部位的生物量变化进行监测。同时，酶解法是一种有效的植物原料解聚技术。我们结合我们的MMCD数据库和R-核磁共振软件技术，提出了一个利用各种一维和二维核磁共振技术预测玉米秸秆酶产物的模型。