METABOLITE CHANGES IN E COLI STRAINS EVOLVED TO BE RADIATION RESISTANT

大肠杆菌菌株的代谢物变化进化为抗辐射性

• 批准号: 8361207
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 0.17万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361207
• 关键词: Escherichia coli; Funding; Gene Expression Profile; Genes; Genomics; Glutamates; Grant; Ionizing radiation; Measures; National Center for Research Resources; Principal Investigator; Putrescine; Radiation; Research; Research Infrastructure; Resistance; Resources; Serine; Source; United States National Institutes of Health; cost; glucose transport; radiation resistance

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To understand the phenomenon of radiation resistance, we have generated several strains of E. coli that were made radiation resistant by repeated adaptation to ionizing radiation. Genomic sequencing and transcriptome analysis show alterations in genes involved in glutamate, putrescine, serine, and glucose transport in the evolved strains. We wish to use FMQ by NMR to measure differences in these strains as compared to the wild-type to elaborate on these previous results.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 为了了解辐射抗性的现象，我们已经产生了几株E。通过反复适应电离辐射而具有抗辐射能力的大肠杆菌。 基因组测序和转录组分析显示，在进化菌株中参与谷氨酸，腐胺，丝氨酸和葡萄糖转运的基因发生了改变。 我们希望通过NMR使用FMQ来测量这些菌株与野生型相比的差异，以详细说明这些先前的结果。