METABOLITE CHANGES IN E COLI STRAINS EVOLVED TO BE RADIATION RESISTANT

大肠杆菌菌株的代谢物变化进化为抗辐射性

• 批准号: 8361207
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 0.17万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361207
• 关键词: Escherichia coli; Funding; Gene Expression Profile; Genes; Genomics; Glutamates; Grant; Ionizing radiation; Measures; National Center for Research Resources; Principal Investigator; Putrescine; Radiation; Research; Research Infrastructure; Resistance; Resources; Serine; Source; United States National Institutes of Health; cost; glucose transport; radiation resistance

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To understand the phenomenon of radiation resistance, we have generated several strains of E. coli that were made radiation resistant by repeated adaptation to ionizing radiation. Genomic sequencing and transcriptome analysis show alterations in genes involved in glutamate, putrescine, serine, and glucose transport in the evolved strains. We wish to use FMQ by NMR to measure differences in these strains as compared to the wild-type to elaborate on these previous results.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 为了理解辐射抗性的现象，我们已经产生了几种大肠杆菌菌株，这些菌株通过反复适应电离辐射而产生了抗辐射。 基因组测序和转录组分析表明，进化菌株中与谷氨酸，腐蚀，丝氨酸和葡萄糖转运有关的基因的改变。 与野生型相比，我们希望使用NMR使用NMR的FMQ来测量这些菌株的差异，以详细说明这些先前的结果。