B BURGDORFERI FLAGELLA

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 运动性是细菌致病机制的重要组成部分，细菌鞭毛马达被认为是实现这一目的的最熟练的生物机器。质子通过马达的流入产生逆时针或顺时针鞭毛旋转，分别导致平移运动或“翻滚”。尽管经过几十年的研究，鞭毛马达旋转的精确机制仍然是一个谜。建议的目的是提供最详细的结构洞察鞭毛电机使用高通量冷冻电子断层扫描（cryo-ET），并将此信息应用到一个可测试的模型鞭毛旋转和电机反转。这项工作将反过来提供致病性和非致病性细菌的运动机制的分子基础。 伯氏疏螺旋体非常适合这种类型的分析，因为1）它的小直径（约0.2微米），这是高分辨率冷冻ET所必需的; 2）约3，600转座子突变体的可用性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Motility is an important component of the pathogenesis of bacteria and the bacterial flagellar motor is considered to be the most proficient biological machine for this purpose. The influx of protons through the motor produces either counterclockwise or clockwise flagellar rotation, resulting in translational motion or 'tumbling', respectively. Despite decades of research, the precise mechanism of flagellar motor rotation has remained a mystery. The purpose of proposal is to provide the most detailed structural insight into the flagellar motor using high-throughput cryo-electron tomography (cryo-ET), and to apply this information to a testable model of flagellar rotation and motor reversal. This work will in turn provide the molecular basis on the mechanism of motility in both pathogenic and nonpathogenic bacteria. Borrelia burgdorferi is ideally suited for this type of analysis because of 1) its small diameter (~0.2 ¿m), which is necessary for high resolution cryo-ET; and 2) the availability of ~3,600 transposon mutants.