POST-TRANSLATIONAL MODIFICATION OF SPD-2/5 AND SAS5/6
SPD-2/5 和 SAS5/6 的翻译后修饰
基本信息
- 批准号:8171369
- 负责人:
- 金额:$ 0.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-01 至 2011-08-31
- 项目状态:已结题
- 来源:
- 关键词:AnimalsBiological AssayCellsCentrosomeCollaborationsComputer Retrieval of Information on Scientific Projects DatabaseDefectDevelopmentFundingGoalsGrantHumanIn VitroInstitutionMass Spectrum AnalysisMicrotubule-Organizing CenterMicrotubulesMitoticMitotic spindleMorphologyNeoplasm MetastasisPhosphorylationPhosphotransferasesPhysiologicalPost-Translational Protein ProcessingProteinsProteomicsResearchResearch PersonnelResourcesSiteSourceUnited States National Institutes of Healthaurora-A kinaseexperiencein vivotumor
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Centrosomes, the major microtubule organizing centers in animal cells, perform critical
functions in cell organization, polarity and division. Abnormalities in centrosome number, size
and morphology have been observed in nearly all human tumor types.
Depletion of four proteins (SPD-2, SPD-5, PLK-1 and Aurora-A kinase (AIR-
1)) was shown to have severe defects in the recruitment of pericentriolar material (PCM).
This recruitment defect results in centrosomes that are unable to nucleate the proper
number of microtubules and ultimately experience catastrophic mitotic defects as proper
mitotic spindles do not form.
Our goal is try to understand the mechanistic relationship between SPD-2, SPD-5, PLK-1 and
AIR-1 by assessing the importance of the kinase activity of AIR-1 and PLK-1 for centrosome
assembly, and by using a combination of in vitro and in vivo approaches to determine if SPD-
2 and/or SPD-5 are activators or susbstrates of Aurora-A kinase.
Through our collaboration with The Center for Physiological Proteomics (CPP), we will use
mass spectrometry after in vitro kinase assays of SPD-5 and SPD-2 with both kinases AIR-1
and PLK-1 to identify the sites that are modified by phosphorylation.
The contribution from CPP will therefore be crucial to understanding centrosome assembly
and function will therefore impact on our understanding of cancer, metastasis and
development.
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者所在的机构。
中心体是动物细胞中主要的微管组织中心,
在细胞组织、极性和分裂中起作用。中心体数目、大小
在几乎所有的人类肿瘤类型中都观察到了肿瘤的形态学变化。
四种蛋白质(SPD-2、SPD-5、PLK-1和Aurora-A激酶(AIR-1))的消耗
1))被证明有严重的缺陷,在招聘的pericentriolar材料(PCM)。
这种募集缺陷导致中心体不能使适当的细胞核化,
数量的微管,并最终经历灾难性的有丝分裂缺陷作为适当的
不形成有丝分裂纺锤体。
我们的目标是试图了解SPD-2,SPD-5,PLK-1和
通过评估AIR-1和PLK-1激酶活性对中心体的重要性,
组装,并通过使用体外和体内方法的组合来确定SPD-
2和/或SPD-5是Aurora-A激酶的激活剂或底物。
通过与生理蛋白质组学中心(CPP)的合作,我们将使用
用两种激酶AIR-1对SPD-5和SPD-2进行体外激酶测定后的质谱分析
和PLK-1来鉴定被磷酸化修饰的位点。
因此,CPP的贡献对于理解中心体组装至关重要
因此,它将影响我们对癌症、转移和
发展
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Karen F Oegema其他文献
Karen F Oegema的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Karen F Oegema', 18)}}的其他基金
IDENTIFICATION OF C05C89 INTERACTING PROTEINS
C05C89 相互作用蛋白的鉴定
- 批准号:
8171386 - 财政年份:2010
- 资助金额:
$ 0.47万 - 项目类别:
IDENTIFICATION OF RHOGAP INTERACTING PROTEINS
RHOGAP 相互作用蛋白的鉴定
- 批准号:
8171422 - 财政年份:2010
- 资助金额:
$ 0.47万 - 项目类别:
HIGH-RESOLUTION PHENOTYPIC PROFILING BASED ON THE COMPLEX ARCHITECTURE OF THE C
基于 C 复杂结构的高分辨率表型分析
- 批准号:
8171265 - 财政年份:2010
- 资助金额:
$ 0.47万 - 项目类别:
相似海外基金
Establishment of a new biological assay using Hydra nematocyst deployment
利用水螅刺丝囊部署建立新的生物测定方法
- 批准号:
520728-2017 - 财政年份:2017
- 资助金额:
$ 0.47万 - 项目类别:
University Undergraduate Student Research Awards
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10368760 - 财政年份:2017
- 资助金额:
$ 0.47万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10669539 - 财政年份:2017
- 资助金额:
$ 0.47万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9570142 - 财政年份:2017
- 资助金额:
$ 0.47万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9915803 - 财政年份:2017
- 资助金额:
$ 0.47万 - 项目类别:
COVID-19 Supplemental work: POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER).
COVID-19 补充工作:用于确定组织特异性吸收电离辐射剂量的护理点生物测定(生物剂量计)。
- 批准号:
10259999 - 财政年份:2017
- 资助金额:
$ 0.47万 - 项目类别:
Drug discovery based on a new biological assay system using Yeast knock-out strain collection
基于使用酵母敲除菌株收集的新生物测定系统的药物发现
- 批准号:
21580130 - 财政年份:2009
- 资助金额:
$ 0.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2005
- 资助金额:
$ 0.47万 - 项目类别:
Postdoctoral Fellowships
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2004
- 资助金额:
$ 0.47万 - 项目类别:
Postdoctoral Fellowships