HEARING IN MICE AND CHICKENS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们将继续研究在哺乳动物耳蜗核的兴奋性突触输入消除后导致神经元死亡(或存活)的细胞间和细胞内信号通路。目前赠款期间的一项主要举措是使用现代遗传分析方法开始评估新生动物和成年动物对小鼠耳蜗核传入剥夺(一个关键期)的不同反应的生物学机制。基因芯片技术被用来筛选在外周剥夺的不同敏感期期间在耳蜗核中差异结构性表达的基因，以及在外周剥夺后的头48小时内，在这一关键时期与之后的关键时期中差异调控的基因(Harris等人，2005；2006；2008)。这些研究中最有趣和最重要的发现之一是，在传入依赖神经元存活(P2-P11；P=出生后一天)和传入之间的转变过程中，转录发生了巨大的变化 独立生存(&gt；P14)，这与听力开始密切相关。但是，在听力成熟的主要时期P14和P21之间，mRNA的表达基本上没有明显的变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will continue investigating the intercellular and intracellular signaling pathways that lead to neuronal death (or survival) following the elimination of excitatory synaptic input to the mammalian cochlear nucleus. A major initiative during the current grant period has been to use modern genetic analysis methods to begin assessing the biological mechanisms underlying differential responses of neonatal and adult animals to afferent deprivation (a critical period) of the mouse cochlear nucleus. Gene microarray technology was used to screen for genes that are differentially constitutively expressed in the cochlear nucleus during periods of differing susceptibility to peripheral deprivation and genes that are differentially regulated during vs. after this critical period in the first 48 hrs following peripheral deprivation (Harris et al., 2005; 2006; 2008). One of the most fascinating and important findings of these studies was that there is an enormous change in transcription during the transition between afferent dependent neuron survival (P2-P11; P=postnatal day) and afferent independent survival (>P14), which corresponds closely with the onset of hearing. But, there is essentially no significant change in mRNA expression between P14 and P21, the major period of hearing maturation.