HEARING IN MICE AND CHICKENS

小鼠和鸡的听力

• 批准号: 8171351
• 负责人: Edwin W Rubel
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171351
• 关键词: Biological; Cessation of life; Chickens; Cochlear nucleus; Computer Retrieval of Information on Scientific Projects Database; Funding; Genes; Genetic Transcription; Grant; Hearing; Institution; Lead; Methods; Microarray Analysis; Mus; Neonatal; Neurons; Peripheral; Predisposition; Research; Research Personnel; Resources; Signal Pathway; Source; Synapses; United States National Institutes of Health; critical period; deprivation; fascinate; genetic analysis; mRNA Expression; mature animal; postnatal; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will continue investigating the intercellular and intracellular signaling pathways that lead to neuronal death (or survival) following the elimination of excitatory synaptic input to the mammalian cochlear nucleus. A major initiative during the current grant period has been to use modern genetic analysis methods to begin assessing the biological mechanisms underlying differential responses of neonatal and adult animals to afferent deprivation (a critical period) of the mouse cochlear nucleus. Gene microarray technology was used to screen for genes that are differentially constitutively expressed in the cochlear nucleus during periods of differing susceptibility to peripheral deprivation and genes that are differentially regulated during vs. after this critical period in the first 48 hrs following peripheral deprivation (Harris et al., 2005; 2006; 2008). One of the most fascinating and important findings of these studies was that there is an enormous change in transcription during the transition between afferent dependent neuron survival (P2-P11; P=postnatal day) and afferent independent survival (>P14), which corresponds closely with the onset of hearing. But, there is essentially no significant change in mRNA expression between P14 and P21, the major period of hearing maturation.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们将继续研究细胞间和细胞内信号通路，导致神经元死亡（或生存）后，兴奋性突触输入到哺乳动物耳蜗核消除。在目前的赠款期间的一个主要举措是使用现代遗传分析方法，开始评估新生儿和成年动物的传入剥夺（一个关键时期）的小鼠耳蜗核的差异反应的生物学机制。基因微阵列技术用于筛选在对外周剥夺的不同易感性的时期期间在耳蜗核中差异组成型表达的基因，以及在外周剥夺后的前48小时内在该关键时期期间与之后差异调节的基因（Harris et al.，2005年; 2006年; 2008年）。这些研究中最有趣和重要的发现之一是，在传入依赖神经元存活（P2-P11; P=出生后）和传入依赖神经元存活（P2-P11; P=出生后）之间的过渡期间，转录发生了巨大的变化。 独立存活（>P14），这与听力的开始密切相关。但在听觉发育的主要时期P14和P21之间，mRNA的表达基本上没有明显变化。