STRUCTURE OF RM-Q COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 衍射数据将从对嗜肺军团菌IVb型分泌系统的功能至关重要的蛋白质复合体中收集。T4bSS是传染性和细菌复制所必需的。嗜肺军团菌感染会导致衰弱的肺炎，在免疫系统减弱的人中可能是致命的。这在医院环境中尤其危险，例如移植病房和烧伤病房。这些晶体包含ICMR相互作用区的1：1复合体，表示为Rm，以及完整的IcmQ蛋白。ICMR的存在可能会阻止IcmQ的功能，并作为伴侣阻止聚集。我们此行的目标是收集更高分辨率的原生数据集(超过2.8A)。全α螺旋复合体大小约为24 kDa，我们设计了一个含三个硒蛋氨酸的复合体。我们将从Se-Met衍生晶体中收集多波长的MAD数据集。这将使我们能够解决结构，然后设计突变来测试这个关键复合体的功能

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Diffraction data will be collected from a complex of proteins that are critical to the function of a Type IVb secretion system in Legionella pneumophila. The T4bSS is required for infectivity and bacterial replication. Legionella pneumophila infections lead to a debilitating pneumonia that can be fatal in persons with a weakened immune system. This is particularly dangerous in hospital settings, such as transplant and burn wards. The crystals contain a 1:1 complex of the interacting region of IcmR, denoted Rm, and the full IcmQ proteins. The presence of IcmR likely blocks the function of IcmQ and acts as a chaperone to prevent aggregation. Our goal in this trip is to collect a higher resolution native data set (beyond 2.8A). The all alpha helical complex is ~24 kDa and we have engineered a complex with three Seleno-methionines. We will collect a multi-wavelength MAD dataset from Se-Met derivative crystals. This will allow us to solve the structure and then design mutations to test the function of this critical complex