STRUCTURE OF RM-Q COMPLEX

RM-Q复合物的结构

• 批准号: 8170588
• 负责人: CHRISTOPHER W AKEY
• 金额: $ 0.56万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170588
• 关键词: Burn injury; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; Engineering; Funding; Goals; Grant; Hospitals; Immune system; Institution; Lead; Legionella pneumophila; Legionnaires' Disease; Methionine; Molecular Chaperones; Mutation; Persons; Pneumonia; Proteins; Research; Research Personnel; Resolution; Resources; Source; Structure; System; Testing; Transplantation; United States National Institutes of Health; design; meetings; prevent; protein complex; ward

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Diffraction data will be collected from a complex of proteins that are critical to the function of a Type IVb secretion system in Legionella pneumophila. The T4bSS is required for infectivity and bacterial replication. Legionella pneumophila infections lead to a debilitating pneumonia that can be fatal in persons with a weakened immune system. This is particularly dangerous in hospital settings, such as transplant and burn wards. The crystals contain a 1:1 complex of the interacting region of IcmR, denoted Rm, and the full IcmQ proteins. The presence of IcmR likely blocks the function of IcmQ and acts as a chaperone to prevent aggregation. Our goal in this trip is to collect a higher resolution native data set (beyond 2.8A). The all alpha helical complex is ~24 kDa and we have engineered a complex with three Seleno-methionines. We will collect a multi-wavelength MAD dataset from Se-Met derivative crystals. This will allow us to solve the structure and then design mutations to test the function of this critical complex

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 将从对嗜肺军团菌中IVb型分泌系统功能至关重要的蛋白质复合物中收集衍射数据。T4 bSS是感染性和细菌复制所必需的。 嗜肺军团菌感染导致虚弱性肺炎，对免疫系统较弱的人来说可能是致命的。这在医院环境中特别危险，例如移植和烧伤病房。晶体包含IcmR相互作用区域（表示为Rm）和完整IcmQ蛋白质的1：1复合物。IcmR的存在可能阻断IcmQ的功能，并作为分子伴侣防止聚集。我们此行的目标是收集更高分辨率的原生数据集（超过2.8A）。全α螺旋复合物为~24 kDa，并且我们已经工程化了具有三个硒代甲硫氨酸的复合物。 我们将从Se-Met衍生物晶体中收集多波长MAD数据集。这将使我们能够解决结构，然后设计突变来测试这个关键复合体的功能