FAST IMAGING METHODS FOR HYPERPOLARIZED NUCLEI

超极化核的快速成像方法

• 批准号: 8171675
• 负责人: James A Bankson
• 金额: $ 0.52万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171675
• 关键词: Acceleration; Address; Algorithms; Animal Cancer Model; Animals; Binding; Cell Nucleus; Chemical Shift Imaging; Clinical; Computer Retrieval of Information on Scientific Projects Database; Detection; Development; Engineering; Ensure; Evaluation; Funding; Goals; Grant; Home environment; Hostage; Image; Imaging technology; Institution; Kinetics; Label; Lead; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Malignant Neoplasms; Measurement; Measures; Metabolic; Methods; Phase; Physics; Physiologic pulse; Play; Protons; Pyruvate; Pyruvates; Rattus; Research; Research Infrastructure; Research Institute; Research Personnel; Resolution; Resources; Role; Science; Scientist; Signal Transduction; Source; Surface; System; Testing; Texas; Translations; United States National Institutes of Health; University of Texas M D Anderson Cancer Center; Validation; anticancer research; cancer care; cancer therapy; clinical care; data acquisition; detector; glucose analog; image reconstruction; imaging modality; improved; in vivo; instrumentation; novel diagnostics; novel strategies; pre-clinical; reconstruction; research study; response; spectroscopic imaging; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of this project is to develop a robust set of instrumentation and methods that facilitate the translation of new discoveries into powerful tools for cancer research and clinical cancer care. The ultimate potential of hyperpolarized (HP) agents is fully realized when these agents are used to probe molecular interactions of cancer in vivo. Small animal models of cancer play a crucial role in the evaluation and validation of novel diagnostic agents and in the discovery and optimization of new approaches to cancer therapy. Unfortunately, the constraints inherent to detection of HP agents are unique and the limited availability of polarizing systems to date has restricted the development of imaging technologies and instrumentation that are optimized for that purpose. Lack of such infrastructure, in turn, slows the demonstration of new applications in vivo and tempers the rate at which new discoveries can progress to clinical care. Significant opportunities currently exist to address these needs and influence the development of imaging technologies and instrumentation for HP measurements in both experimental and clinical settings. Klaes Golman first demonstrated metabolic imaging using HP 13C-labeled pyruvate in rats using a 1H/13C dual-tuned volume coil and a standard chemical-shift imaging (CSI) sequence with centric k-space encoding. Kevin Brindle's group at the Cambridge Research Institute has shown an early indication of response to therapy in the kinetics of HP-[1-13C]-pyruvate when measured using a standard CSI sequence and a surface coil (which aided signal localization) that was tuned to 13C. Scientists at UCSF and GE have developed an efficient double spin-echo echo-planar spectroscopic imaging (EPSI) sequence for HP-13C measurements and have applied compressed sensing to accelerate acquisitions made using a home-made 1H/13C dual-tuned volume coil. Leupold et al. have employed an iterative Dixon-like reconstruction to minimize encoding in the spectral domain for steady-state imaging of proton and HP-13C-CSI using a quadrature 13C surface coil. None but the most basic (and least efficient) of these sequences are widely available to researchers that seek to employ HP-13C methods in vivo, and only one of these groups has utilized a commercially available coil rather than purpose-built detectors that are tailored for specific experimental conditions. Notably absent from all of these approaches is the use of multichannel 13C capabilities to further accelerate data acquisition by parallel encoding. Increases in sensitivity and signal localization using arrays could lead to substantial improvements in spatial and temporal resolution when measuring HP-13C in vivo. This project leverages the expertise of colleagues at The University of Texas M.D. Anderson Cancer Center in imaging physics, experimental MRI, systems engineering, and small animal imaging for the development of coils, sequences, and reconstruction algorithms that are tailored to the unique constraints imposed by measurements of hyperpolarized media. Coils and arrays that are optimized and tailored for specific measurements will improve sensitivity and support efficient image acquisition methods. Fast and efficient pulse sequences will gather the most information from the decaying signal pool and improve spatial and temporal resolution. Acceleration via parallel imaging will further preserve signal by enabling reconstruction from fewer signal excitations and phase-encoding repetitions. Thorough characterization of coils and sequences will yield a robust platform for HP-CSI and provide a direct conduit for HP-CSI in small animal models of cancer. These goals will be achieved through three specific aims: Aim 1: Coils and arrays for quantitative MRSI of hyperpolarized nuclei. This aim will ensure that science is not held hostage by lack of commercially available instrumentation. Coils that support proton and hyperpolarized nuclei will be optimized, characterized, and provided to partners in this Texas network. The capability of multinuclear arrays to further improve the quality and resolution of HP-CSI will be tested. Aim 2: Sequences for rapid and efficient encoding and reconstruction. Efficient signal encoding and rapid imaging sequences improve the spatial and temporal resolution at which measurements can be made from decaying signal. This aim will focus on two classes of experiments: one for HP-13C observe, and one for rapid 1H imaging following polarization transfer (13C to 1H) in HP glucose analogues. Parallel and constrained image reconstruction methods will be integrated into rapid HP-CSI and HP-MRI sequences and their potential for acceleration will be tested. Aim 3: Integration of hyperpolarized measurements into preclinical cancer research. Optimized single-channel and parallel imaging methods for hyperpolarized experiments will be integrated into ongoing research involving small animal models of cancer at MDACC and UTSW, with provisions to increase study size and evaluate the repeatability of these methods.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的总体目标是开发一套强大的仪器和方法，以促进将新发现转化为癌症研究和临床癌症护理的强大工具。当超极化(HP)试剂被用于探测体内癌症的分子相互作用时，这些试剂的最终潜力被充分发挥出来。癌症的小动物模型在评估和验证新的诊断试剂以及发现和优化癌症治疗的新方法方面发挥着至关重要的作用。不幸的是，检测HP试剂所固有的限制是独一无二的，而且到目前为止，偏振系统的有限可用性限制了为此目的优化的成像技术和仪器的发展。缺乏这样的基础设施，反过来又会减缓体内新应用的展示，并减缓新发现进入临床治疗的速度。目前存在着大量的机会来满足这些需求，并影响在实验和临床环境中用于HP测量的成像技术和仪器的发展。 Klaes Golman首次展示了使用HP 13C标记的丙酮酸在大鼠身上进行的代谢成像，该成像使用了一个1H/13C双调谐体积线圈和具有中心k空间编码的标准化学位移成像(CSI)序列。剑桥研究所的Kevin Brindle团队在使用标准CSI序列和调谐到13C的表面线圈(有助于信号定位)测量HP-[1-13C]-丙酮酸的动力学时，已经显示出治疗反应的早期迹象。加州大学旧金山分校和通用电气的科学家开发了一种用于HP-13C测量的高效双自旋回波平面光谱成像(EPSI)序列，并应用压缩传感技术加快了使用国产1H/13C双调谐卷线圈进行的采集。Leupold等人。采用了迭代Dixon式重建，以最大限度地减少光谱域中的编码，以便使用正交13C表面线圈对质子和HP-13C-CSI进行稳态成像。对于寻求在体内使用HP-13C方法的研究人员来说，除了最基本的(也是最低效率的)这些序列，没有一个是广泛可用的，而且这些小组中只有一个使用了商业上可用的线圈，而不是为特定实验条件量身定做的专门建造的探测器。值得注意的是，所有这些方法都没有使用多通道13C功能，以通过并行编码进一步加速数据采集。在活体测量HP-13C时，使用阵列提高灵敏度和信号定位可以大幅提高空间和时间分辨率。 该项目利用德克萨斯大学M.D.安德森癌症中心同事在成像物理、实验核磁共振、系统工程和小动物成像方面的专业知识，开发线圈、序列和重建算法，以满足超极化介质测量施加的独特约束。为特定测量优化和定制的线圈和阵列将提高灵敏度并支持高效的图像采集方法。快速而高效的脉冲序列将从衰落的信号池中收集最多的信息，并提高空间和时间分辨率。通过并行成像的加速将通过从更少的信号激励和相位编码重复进行重建来进一步保存信号。对线圈和序列的彻底表征将为HP-CSI提供一个强大的平台，并为HP-CSI在小动物癌症模型中提供直接管道。这些目标将通过三个具体目标来实现： 目的1：用于超极化核磁共振成像定量的线圈和阵列。这一目标将确保科学不会因为缺乏商业仪器而被扣为人质。支持质子和超极化原子核的线圈将被优化、表征并提供给德克萨斯州这个网络中的合作伙伴。将测试多核阵列进一步提高HP-CSI质量和分辨率的能力。 目标2：用于快速高效编码和重建的序列。有效的信号编码和快速成像序列提高了从衰减信号进行测量的空间和时间分辨率。这一目标将集中于两类实验：一类用于HP-13C观察，另一类用于HP葡萄糖类似物中极化转移(13C到1H)后的快速1H成像。并行和约束图像重建方法将被集成到快速HP-CSI和HP-MRI序列中，并将测试它们的加速潜力。 目标3：将超极化测量整合到临床前癌症研究中。用于超极化实验的优化的单通道和并行成像方法将被整合到MDACC和UTSW正在进行的涉及癌症小动物模型的研究中，并规定扩大研究规模并评估这些方法的可重复性。