FAST IMAGING METHODS FOR HYPERPOLARIZED NUCLEI

超极化核的快速成像方法

• 批准号: 8363924
• 负责人: James A Bankson
• 金额: $ 0.8万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363924
• 关键词: Acceleration; Address; Algorithms; Animal Cancer Model; Animals; Binding; Cell Nucleus; Chemical Shift Imaging; Clinical; Detection; Development; Engineering; Ensure; Evaluation; Funding; Goals; Grant; Home environment; Hostage; Image; Imaging technology; Kinetics; Label; Lead; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Malignant Neoplasms; Measurement; Measures; Metabolic; Metabolism; Methods; National Center for Research Resources; Phase; Physics; Physiologic pulse; Play; Principal Investigator; Protons; Pyruvate; Rattus; Research; Research Infrastructure; Research Institute; Research Personnel; Resolution; Resources; Role; Science; Scientist; Signal Transduction; Source; Surface; System; Testing; Texas; Translations; United States National Institutes of Health; University of Texas M D Anderson Cancer Center; Validation; anticancer research; cancer care; cancer therapy; clinical care; cost; data acquisition; detector; glucose analog; image reconstruction; imaging modality; improved; in vivo; instrumentation; novel diagnostics; novel strategies; pre-clinical; reconstruction; research study; response; spectroscopic imaging; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The overall goal of this project is to develop a robust set of instrumentation and methods that facilitate the translation of new discoveries into powerful tools for cancer research and clinical cancer care. The ultimate potential of hyperpolarized (HP) agents is fully realized when these agents are used to probe molecular interactions of cancer in vivo. Small animal models of cancer play a crucial role in the evaluation and validation of novel diagnostic agents and in the discovery and optimization of new approaches to cancer therapy. Unfortunately, the constraints inherent to detection of HP agents are unique and the limited availability of polarizing systems to date has restricted the development of imaging technologies and instrumentation that are optimized for that purpose. Lack of such infrastructure, in turn, slows the demonstration of new applications in vivo and tempers the rate at which new discoveries can progress to clinical care. Significant opportunities currently exist to address these needs and influence the development of imaging technologies and instrumentation for HP measurements in both experimental and clinical settings. Klaes Golman first demonstrated metabolic imaging using HP 13C-labeled pyruvate in rats using a 1H/13C dual-tuned volume coil and a standard chemical-shift imaging (CSI) sequence with centric k-space encoding. Kevin Brindle's group at the Cambridge Research Institute has shown an early indication of response to therapy in the kinetics of HP-[1-13C]-pyruvate when measured using a standard CSI sequence and a surface coil (which aided signal localization) that was tuned to 13C. Scientists at UCSF and GE have developed an efficient double spin-echo echo-planar spectroscopic imaging (EPSI) sequence for HP-13C measurements and have applied compressed sensing to accelerate acquisitions made using a home-made 1H/13C dual-tuned volume coil. Leupold et al. have employed an iterative Dixon-like reconstruction to minimize encoding in the spectral domain for steady-state imaging of proton and HP-13C-CSI using a quadrature 13C surface coil. None but the most basic (and least efficient) of these sequences are widely available to researchers that seek to employ HP-13C methods in vivo, and only one of these groups has utilized a commercially available coil rather than purpose-built detectors that are tailored for specific experimental conditions. Notably absent from all of these approaches is the use of multichannel 13C capabilities to further accelerate data acquisition by parallel encoding. Increases in sensitivity and signal localization using arrays could lead to substantial improvements in spatial and temporal resolution when measuring HP-13C in vivo. This project leverages the expertise of colleagues at The University of Texas M.D. Anderson Cancer Center in imaging physics, experimental MRI, systems engineering, and small animal imaging for the development of coils, sequences, and reconstruction algorithms that are tailored to the unique constraints imposed by measurements of hyperpolarized media. Coils and arrays that are optimized and tailored for specific measurements will improve sensitivity and support efficient image acquisition methods. Fast and efficient pulse sequences will gather the most information from the decaying signal pool and improve spatial and temporal resolution. Acceleration via parallel imaging will further preserve signal by enabling reconstruction from fewer signal excitations and phase-encoding repetitions. Thorough characterization of coils and sequences will yield a robust platform for HP-CSI and provide a direct conduit for HP-CSI in small animal models of cancer. These goals will be achieved through three specific aims: Aim 1: Coils and arrays for quantitative MRSI of hyperpolarized nuclei. This aim will ensure that science is not held hostage by lack of commercially available instrumentation. Coils that support proton and hyperpolarized nuclei will be optimized, characterized, and provided to partners in this Texas network. The capability of multinuclear arrays to further improve the quality and resolution of HP-CSI will be tested. Aim 2: Sequences for rapid and efficient encoding and reconstruction. Efficient signal encoding and rapid imaging sequences improve the spatial and temporal resolution at which measurements can be made from decaying signal. This aim will focus on two classes of experiments: one for HP-13C observe, and one for rapid 1H imaging following polarization transfer (13C to 1H) in HP glucose analogues. Parallel and constrained image reconstruction methods will be integrated into rapid HP-CSI and HP-MRI sequences and their potential for acceleration will be tested. Aim 3: Integration of hyperpolarized measurements into preclinical cancer research. Optimized single-channel and parallel imaging methods for hyperpolarized experiments will be integrated into ongoing research involving small animal models of cancer at MDACC and UTSW, with provisions to increase study size and evaluate the repeatability of these methods.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 该项目的总体目标是开发一套强大的仪器和方法，促进将新发现转化为癌症研究和临床癌症护理的强大工具。超极化（HP）试剂的最终潜力完全实现时，这些试剂用于探测体内癌症的分子相互作用。癌症的小动物模型在评估和验证新型诊断剂以及发现和优化癌症治疗新方法方面发挥着至关重要的作用。不幸的是，HP试剂检测的固有约束是独特的，并且迄今为止偏振系统的有限可用性限制了为此目的优化的成像技术和仪器的发展。缺乏这样的基础设施，反过来，减缓了体内新应用的演示，并缓和了新发现进展到临床护理的速度。目前存在着重大的机会，以满足这些需求，并影响成像技术和仪器的发展HP测量在实验和临床设置。 Klaes Golman首先使用1H/13 C双调谐体积线圈和具有中心k空间编码的标准化学位移成像（CSI）序列在大鼠中使用HP 13 C标记的丙酮酸盐进行代谢成像。剑桥研究所的Kevin Brindle小组在使用标准CSI序列和调谐至13 C的表面线圈（辅助信号定位）测量时，显示了HP-[1- 13 C]-丙酮酸动力学对治疗反应的早期迹象。加州大学旧金山分校和通用电气公司的科学家已经开发出一种有效的双自旋回波回波平面光谱成像（EPSI）序列，用于HP-13 C测量，并应用压缩传感来加速使用自制的1H/13 C双调谐体积线圈进行的采集。Leupold等人已经采用迭代Dixon样重建来最小化使用正交13 C表面线圈的质子和HP-13 C-CSI的稳态成像的谱域中的编码。这些序列中只有最基本（和最低效）的序列可广泛用于寻求在体内采用HP-13 C方法的研究人员，并且这些组中只有一个组使用了市售的线圈，而不是针对特定实验条件定制的专用检测器。值得注意的是，所有这些方法都没有使用多通道13 C功能来通过并行编码进一步加速数据采集。使用阵列的灵敏度和信号定位的增加可能导致在体内测量HP-13 C时空间和时间分辨率的实质性改善。 该项目利用了德克萨斯大学医学博士的同事们的专业知识。安德森癌症中心在成像物理学，实验MRI，系统工程和小动物成像的线圈，序列和重建算法的开发，是专为超极化介质的测量所施加的独特约束。针对特定测量进行优化和定制的线圈和阵列将提高灵敏度并支持高效的图像采集方法。快速有效的脉冲序列将从衰减信号池中收集最多的信息，并提高空间和时间分辨率。经由并行成像的加速将通过使得能够从较少的信号激励和相位编码重复进行重建来进一步保留信号。线圈和序列的彻底表征将产生用于HP-CSI的稳健平台，并在癌症的小动物模型中提供用于HP-CSI的直接管道。这些目标将通过三个具体目标实现： 目的1：超极化核定量MRSI线圈和阵列。这一目标将确保科学不因缺乏商业上可用的仪器而受到束缚。支持质子和超极化核的线圈将被优化，表征，并提供给这个德克萨斯网络的合作伙伴。将测试多核阵列进一步提高HP-CSI质量和分辨率的能力。 目标2：用于快速有效编码和重建的序列。有效的信号编码和快速成像序列提高了空间和时间分辨率，在该分辨率下可以从衰减信号进行测量。这一目标将集中在两类实验：一类是HP-13 C观察，另一类是HP葡萄糖类似物中极化转移（13 C到1H）后的快速1H成像。并行和约束图像重建方法将被集成到快速HP-CSI和HP-MRI序列中，并将测试其加速潜力。 目标3：将超极化测量整合到临床前癌症研究中。用于超极化实验的优化单通道和平行成像方法将被整合到MDACC和UTSW正在进行的涉及癌症小动物模型的研究中，并规定增加研究规模和评估这些方法的可重复性。