ASSESSMENT OF BRAIN IRON AND NEURONAL INTEGRITY USING NOVEL T1? AND T2? MRI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在这个项目中，我们的目标是建立新的高分辨率磁共振成像(MRI)技术，T1？，作为对大脑中神经元丢失敏感的定量方法。我们将使用单侧6-羟基多巴胺(6-OHDA)PD小鼠模型(纹状体内注射2-6-OHDA/L)来研究T1的预测/识别能力。我们将在无晶状体小鼠中研究T1与神经元密度的功能关系(在无晶状体小鼠中，黑质多巴胺能神经元的缺陷和神经元的生存途径得到了很好的研究)。一旦得到验证，这里提出的技术可以作为一个有价值的工具来研究黑质神经元丢失是否以及如何与帕金森病的进展相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this project we aim to establish novel high-resolution magnetic resonance imaging (MRI) technique, T1¿, as quantitative methods sensitive to neuronal loss in the brain. We will investigate the predictive/discriminatory ability of the T1¿ using a unilateral 6-hydroxydopamine (6-OHDA) PD mouse model (intrastriatal injection of 2 ¿g 6-OHDA/¿L). We will study the functional relationship between T1¿ with neuronal density in aphakia mice (where the deficit of dopaminergic neurons in substantia nigra and the pathways for survival of neurons are well investigated). Once validated, the technique proposed here can be used as a valuable tool to investigate if and how neuronal loss in substantia nigra is correlated with the progression of Parkinson's disease.