ASSESSMENT OF BRAIN IRON AND NEURONAL INTEGRITY USING NOVEL T1? AND T2? MRI

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 在这个项目中，我们的目标是建立新的高分辨率磁共振成像（MRI）技术，T1 <$，作为定量方法敏感的神经元损失在大脑中。我们将使用单侧6-羟基多巴胺（6-OHDA）PD小鼠模型（纹状体内注射2 <$g 6-OHDA/<$L）研究T1 <$的预测/区分能力。我们将研究无晶状体小鼠（其中黑质中多巴胺能神经元的缺陷和神经元存活的途径得到了很好的研究）中T1？与神经元密度之间的功能关系。一旦验证，这里提出的技术可以被用作一个有价值的工具，以调查是否以及如何在黑质神经元的损失与帕金森氏病的进展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this project we aim to establish novel high-resolution magnetic resonance imaging (MRI) technique, T1¿, as quantitative methods sensitive to neuronal loss in the brain. We will investigate the predictive/discriminatory ability of the T1¿ using a unilateral 6-hydroxydopamine (6-OHDA) PD mouse model (intrastriatal injection of 2 ¿g 6-OHDA/¿L). We will study the functional relationship between T1¿ with neuronal density in aphakia mice (where the deficit of dopaminergic neurons in substantia nigra and the pathways for survival of neurons are well investigated). Once validated, the technique proposed here can be used as a valuable tool to investigate if and how neuronal loss in substantia nigra is correlated with the progression of Parkinson's disease.