ASSESSMENT OF BRAIN IRON AND NEURONAL INTEGRITY USING NOVEL T1? AND T2? MRI

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在这个项目中，我们的目标是建立新的高分辨率磁共振成像(MRI)技术，T1？，作为对大脑中神经元丢失敏感的定量方法。我们将使用单侧6-羟基多巴胺(6-OHDA)PD小鼠模型(纹状体内注射2-6-OHDA/L)来研究T1的预测/识别能力。我们将在无晶状体小鼠中研究T1与神经元密度的功能关系(在无晶状体小鼠中，黑质多巴胺能神经元的缺陷和神经元的生存途径得到了很好的研究)。一旦得到验证，这里提出的技术可以作为一个有价值的工具来研究黑质神经元丢失是否以及如何与帕金森病的进展相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In this project we aim to establish novel high-resolution magnetic resonance imaging (MRI) technique, T1¿, as quantitative methods sensitive to neuronal loss in the brain. We will investigate the predictive/discriminatory ability of the T1¿ using a unilateral 6-hydroxydopamine (6-OHDA) PD mouse model (intrastriatal injection of 2 ¿g 6-OHDA/¿L). We will study the functional relationship between T1¿ with neuronal density in aphakia mice (where the deficit of dopaminergic neurons in substantia nigra and the pathways for survival of neurons are well investigated). Once validated, the technique proposed here can be used as a valuable tool to investigate if and how neuronal loss in substantia nigra is correlated with the progression of Parkinson's disease.