PROTEOME AND TRASCRIPTOME ANALYSIS OF HEMORRHAGIC FEVER IN CYNOMOLGUS MACAQUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 丝状病毒马尔堡是一种持久的和隐约可见的生物制剂，适用于几种暴发情况。它们的高死亡率和人与人之间传播的能力，加上缺乏疫苗、治疗和诊断，是自然暴发、输入和生物恐怖主义令人担忧的原因。安哥拉马尔堡疫情的再次出现(世卫组织最新报告)继续提醒我们注意非洲各区域的潜在疾病。由于丝状病毒宿主宿主(S)仍未确定(S)，疫情预测和控制非常困难。由于气雾化病毒具有传染性，只需要少数病毒粒子即可感染，丝状病毒被认为是潜在的生物武器。由于一些储存和创造它们的科学家仍然下落不明，马尔堡病毒代表着当今最严重的生物修复之一。因此，我们迫切需要使生物治疗潜力合理化。 观察到马尔堡出血热的某些暴发会导致不同程度的死亡，这表明毒力更强的菌株可能会改变宿主对感染的反应。宿主的反应非常复杂，涉及大量的细胞类型。我们推测，这些细胞类型中宿主转录组和蛋白质组反应的差异可以用于确定感染的特征，并识别具有改变致病性的马尔堡变异体。这些图谱将在宿主反应计划内的几个分子水平上提供对生物修复潜力的更深层次的理解。此外，我们相信，多因素分析将准确地识别导致生物代谢表型改变的基因改变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The filovirus Marburg is a persistent and looming biothreat agent for several outbreak scenarios. Their high fatality rate and capacity for person to person transmission, combined with a lack of vaccines, therapeutics and diagnostics, are cause for concern in natural outbreaks, importation and bioterrorism. The re-emergences of Marburg in Angola (latest WHO report) continues to remind us of the disease potential in regions of Africa. Since the filoviral host reservoir(s) still remain(s) unidentified, outbreak prediction and control are very difficult. Since aerosolized virus is infectious and only a few virions are required for infectivity, filoviruses are regarded as potential bioweapons. Since some of the stockpiles and the scientists who created them remain unaccounted for, Marburg virus represents one of the most serious biothreats today. Consequently, there is an urgent need for us to rationalize the biothreat potentials. The observation that certain outbreaks of Marburg hemorrhagic fever result in varying degrees of mortality suggests that more virulent strains may alter the host response to infection. The host response is highly complex and involves a large number of cell types. We hypothesize that the differences in host transcriptome and proteome responses within these cell types can be used to characterize infection and identify Marburg variants with altered pathogenicity. These profiles will provide a deeper understanding of the meaning of biothreat potential at several molecular levels within the host response program. Furthermore, we believe that a multifactorial profiling will accurately identify genotypic alterations responsible for altered biothreat phenotype.