THE COMMON MARMOSET AS A MODEL FOR MARBURG HEMORRHAGIC FEVER

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 马尔堡病毒是丝状病毒科的一个成员，在人类和非人类灵长类动物中引起严重的出血热。这是一种新出现的病毒，自1967年首次发现以来，每两年定期报告一次疫情。马尔堡是美国国立卫生研究院A类选择剂，需要最大限度的遏制生物安全设施来操纵活病毒。病毒通过接触受感染个体或动物的血液或体液传播，具有高度传染性。马尔堡病毒感染的最后阶段以发热、出血、肿胀性休克为特征，明显依赖于网状内皮细胞和单核吞噬细胞系统。除了治标措施外，没有有效的治疗方法。 本初步研究的重点是开发一种小型非人灵长类动物马尔堡出血热模型，以研究发病机制、生物威胁潜力和干预措施。该药物在细胞和整个动物水平的发病机制知之甚少。由于目前用于研究病毒的非人灵长类动物的短缺，以及在生物遏制中容纳较大动物所需的大量费用，因此需要开发较小的非人灵长类动物模型来测试候选疫苗和干预策略。普通绒猴已成为传染病研究中一种有价值的非人灵长类动物。它的小尺寸（~ 400 g）及其模仿人类对感染反应的倾向使其成为感染性疾病模型开发的合适候选者。中心假设是，普通绒猴（Callithrix jacchus）在接种马尔堡病毒后，将出现与人类出血热一致的疾病状态和免疫应答。 本研究的成功完成将使我们深入了解绒猴作为马尔堡出血热模型的有用性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Marburg virus is a member of the family Filoviridae and causes severe hemorrhagic fevers in humans and nonhuman primates. It is an emerging virus and outbreaks have been reported periodically at 2-year intervals since its initial identification in 1967. Marburg is a NIH Category A select agent requiring maximum containment biosafety facilities to manipulate live virus. Virus is spread by contact with blood or body fluids from infected individuals or animals and is highly infectious. The final stages of Marburg virus infection are characterized by fever, hemorrhage, hypotensive shock with an apparent dependence on the reticuloendothelial and mononuclear phagocytic cell systems. Apart from palliative measures, there is no effective treatment for the disease. This pilot study focuses on development of a small nonhuman primate model of Marburg Hemorrhagic fever to study pathogenesis, biothreat potential, and interventions. The agent has poorly understood mechanisms of pathogenesis at both the cellular and whole animal level. Because of the shortage of non human primates currently used to study the virus, as well as the large expense required to house larger animals in biocontainment, there is a need to develop smaller non human primate models to test candidate vaccines and intervention strategies. The common marmoset has emerged as a valuable non human primate in infectious disease research. Its small size (-400 g), and its tendency to mimic human responses to infection, make it a suitable candidate for infectious disease model development. The central hypothesis is that the common marmoset (Callithrix jacchus), when inoculated with Marburg virus, will develop a disease state and immune response that is consistent with that seen in human hemorrhagic fever. Successful completion of the study will provide insight into the usefulness of the marmoset as a model for Marburg hemorrhagic fever.