INTRARECTAL TITRATION OF SHIV 162P4 STOCK

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 我们以前报道了一个比较研究的体内感染性和致病性的CCR 5-热带SHIVSF 162 P4在恒河猴和猪尾猕猴。结果表明，恒河猴和猪尾猕猴对SHIVSF 162 P4通过粘膜途径感染的易感性相似。然而，SHIV复制显着更强大的猪尾猕猴比恒河猴。由于持续性病毒感染与中和抗体应答的扩大相关，因此在实现本研究的主要目标后，我们继续监测了受感染猪尾猕猴亚组中病毒序列和中和抗体活性的演变。从这些研究中获得的信息可能有助于建立猕猴模型，以确定产生抗HIV-1的广泛中和抗体所需的因素。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We previously reported a comparative study of the in vivo infectivity and pathogenicity of a CCR5-tropic SHIVSF162 P4 in rhesus and pig-tailed macaques. Results indicate that both rhesus and pig-tailed macaques are similarly susceptible to SHIVSF162 P4 infection by mucosal routes. However, SHIV replication was significantly more robust in pig-tailed macaques than in rhesus. Since, persistent viral infection has been correlated with broadening of neutralizing antibody responses, we continued to monitor the evolution of viral sequences and neutralizing antibody activities in a subset of infected pig-tailed macaques after the primary goal of this study was achieved. Information obtained from these studies may help the establishment of macaque models to determine factors required for the generation of broadly neutralizing antibodies against HIV-1.