INTRARECTAL TITRATION OF SHIV 162P4 STOCK

SHIV 162P4 库存的直肠内滴定

• 批准号: 8172740
• 负责人: Shiu-Lok Hu
• 金额: $ 46.53万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172740
• 关键词: Antibody Formation; Comparative Study; Computer Retrieval of Information on Scientific Projects Database; Evolution; Family suidae; Funding; Generations; Goals; Grant; HIV-1; Infection; Institution; Macaca; Macaca mulatta; Modeling; Monitor; Pathogenicity; Reporting; Research; Research Personnel; Resources; Route; Source; Tail; Titrations; United States National Institutes of Health; Viral; Virus Diseases; in vivo; neutralizing antibody; simian human immunodeficiency virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We previously reported a comparative study of the in vivo infectivity and pathogenicity of a CCR5-tropic SHIVSF162 P4 in rhesus and pig-tailed macaques. Results indicate that both rhesus and pig-tailed macaques are similarly susceptible to SHIVSF162 P4 infection by mucosal routes. However, SHIV replication was significantly more robust in pig-tailed macaques than in rhesus. Since, persistent viral infection has been correlated with broadening of neutralizing antibody responses, we continued to monitor the evolution of viral sequences and neutralizing antibody activities in a subset of infected pig-tailed macaques after the primary goal of this study was achieved. Information obtained from these studies may help the establishment of macaque models to determine factors required for the generation of broadly neutralizing antibodies against HIV-1.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们之前报道了一项 CCR5 趋向性 SHIVSF162 P4 在恒河猴和猪尾猕猴体内感染性和致病性的比较研究。结果表明，恒河猴和猪尾猕猴同样容易通过粘膜途径感染 SHIVSF162 P4。然而，猪尾猕猴中的 SHIV 复制明显强于恒河猴。由于持续性病毒感染与中和抗体反应的扩大有关，因此在实现本研究的主要目标后，我们继续监测一部分受感染猪尾猕猴中病毒序列的演变和中和抗体活性。从这些研究中获得的信息可能有助于建立猕猴模型，以确定产生广泛中和 HIV-1 抗体所需的因素。