INTRARECTAL TITRATION OF SHIV 162P4 STOCK

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们之前报道了CCR5嗜性SHIVSF162 P4在恒河猴和猪尾猕猴体内感染性和致病性的比较研究。结果表明，恒河猴和猪尾猕猴对SHIVSF162 P4通过粘膜途径感染的易感性相似。然而，SIV病毒在猪尾猕猴中的复制明显比在恒河猴中更强劲。由于持续的病毒感染与中和抗体反应的扩大有关，在本研究的主要目标实现后，我们继续监测受感染的猪尾猕猴中病毒序列的进化和中和抗体活性。从这些研究中获得的信息可能有助于建立猕猴模型，以确定产生针对HIV-1的广谱中和抗体所需的因素。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We previously reported a comparative study of the in vivo infectivity and pathogenicity of a CCR5-tropic SHIVSF162 P4 in rhesus and pig-tailed macaques. Results indicate that both rhesus and pig-tailed macaques are similarly susceptible to SHIVSF162 P4 infection by mucosal routes. However, SHIV replication was significantly more robust in pig-tailed macaques than in rhesus. Since, persistent viral infection has been correlated with broadening of neutralizing antibody responses, we continued to monitor the evolution of viral sequences and neutralizing antibody activities in a subset of infected pig-tailed macaques after the primary goal of this study was achieved. Information obtained from these studies may help the establishment of macaque models to determine factors required for the generation of broadly neutralizing antibodies against HIV-1.